Analysis of TLC-Bioautography and TLC-Spot Visualization of <i>Atropa accuminata</i> and <i>Atropa belladonna</i> Extracts as Antioxidant and Antibacterial Agents Against Human Pathogenic Bacteria

Hussain, Saeeda; Babar, Muhammad; S, Kousar; Wazir, Samna; Fareed, Farwa; Latif, Farooq; Andleeb, Saiqa; Ali, Shaukat

doi:10.2174/1573412913666170117162851

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2020

2024

Publication Types

Select...

Book1

Article1

Relationship

Self Cite0

Independent2

Authors

Journals

Cited by 2 publications

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Application of Thin-Layer Chromatography in Quality Control of Chinese Medicines

Chen,

Xu,

Zhao

et al. 2024

Quality Control of Chinese Medicines

View full text Add to dashboard Cite

Application of Thin-Layer Chromatography in Quality Control of Chinese Medicines

Chen,

Xu,

Zhao

et al. 2024

Quality Control of Chinese Medicines

View full text Add to dashboard Cite

TLC Bioautography on Screening of Bioactive Natural Products: An Update Review

Zang

Chen

2020

CAC

View full text Add to dashboard Cite

Background: TLC bioautography is a hyphenated technique combining planar chromatographic separation and in situ biological activity detection. This coupled method has been receiving much attention in screening bio-active natural products because of its properties of being simple, rapid, inexpensive, and effective. Methods: The recent progress in the development of method of TLC bioautography for detecting antimicrobial and enzyme inhibitory activities dating between 2012 and early 2018 has been reviewed. The applications of this method in biological screening of natural products were also presented. Results: Some anaerobic and microaerophilic bacteria and a causative bacterium of tuberculosis have been adopted to TLC direct bioautography. Seven types of enzymes including acetylcholinesterase, glucosidase, lipase, xanthine oxidase, tyrosinase, monoamine oxidase, and dipeptidyl peptidase IV have so far been adopted on TLC bioautography. Its new application in screening antiurolithiatic agents was included. Conclusion: The standard experimental procedures are required for TLC antioxidant and antimicrobial assays. Some new enzymes should be attempted and adopted on TLC bioautography. The existing TLC methods for enzyme inhibition need more application studies to assess their screening capacity in the discovery of active compounds. The GC-MS or LC-MS approaches have gradually been coupled to TLC bioautography for fast structural characterization of active compounds.

show abstract

Analysis of TLC-Bioautography and TLC-Spot Visualization of Atropa accuminata and Atropa belladonna Extracts as Antioxidant and Antibacterial Agents Against Human Pathogenic Bacteria

Cited by 2 publications

References 0 publications

Application of Thin-Layer Chromatography in Quality Control of Chinese Medicines

Application of Thin-Layer Chromatography in Quality Control of Chinese Medicines

TLC Bioautography on Screening of Bioactive Natural Products: An Update Review

Contact Info

Product

Resources

About