The present study focused on the physicochemical characterization and spectrophotometric determination ofthe concentrations of trace elements (Zn, Cu, Fe and Mn) and toxic heavy metals (Cd and Pb) in raw milk samplescollected from various commercial sites of Peshawar city, Pakistan. Evaluation of the nutritional values as well as thepossible health concerns associated with the consumption of contaminated milk shown the average percentagecomposition of protein, fats, solid non-fats, lactose, salts and added water in the milk sample as 2.78, 4.81, 6.41, 3.05,0.47 and 31.1 %, respectively. The values of pH, conductivity, density and freezing point were observed as 6.59, 3.51mS/cm, 1.04 g/mL and -0.3674 ºC at 26 ºC. The physicochemical characteristics such as percentage protein, fat,lactose, salts, water, solid not fat, total solids, pH, conductivity and density were evaluated and found in the safe limitsof the WHO/FAO. The average concentration of trace elements such as zinc and copper were found to be 1.4035 mg/Land 0.2588 mg/L, respectively. The concentration of Zn and Cu was found within permissible limits suggested byWHO. However, the average concentration of Fe, Mn, Cd and Pb were found higher than the permissible limits ofWHO. The observed concentrations were: Fe = 1.5553, Mn = 0.4354, Cd = 0.1865 and Pb = 1.1162 mg/L. Thepresence of the non-essential and hazardous Cd and Pb in milk samples even in lower concentrations may cause severehealth problems since these are the most noxious pollutants due to their non-biodegradable and bio-accumulativenature. Over-dose of Fe and Mn also leads to several health issues. A thorough and more precise investigation of rawmilk consumption in Peshawar is highly recommended to ascertain the actual reasons and sources in the larger publicinterests.