Analysis of Transcriptional Regulatory Regions of The Human p53 Gene in Human Cells Using An EBV-Derived Shuttle Vector

Episomal vectors for gene expression in mammalian cells

“…The development of eukaryotic expression vectors has provided a direct and convenient way of introducing novel genetic information into cultured cells. Heterologous expression of recombinant proteins has subsequently found widespread use for overproduction of therapeutically important proteins and for studies of gene regulation [1–7]. However, integration of foreign DNA into the genome of established cells and organisms may have important consequences, especially when used for gene therapy.…”