Analysis of Translational Activity of Extracts Derived from Oocytes and Eggs of Xenopus laevis

Pain, Virginia M.; Patrick, Tina; Cox, Richard F.; Morley, Simon

doi:10.1385/0-89603-397-x:195

Cited by 1 publication

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References 7 publications

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“…and disassembly (Murray and Kirschner 1989a,b;Murray et al 1989;Glotzer et al 1991;Newmeyer and Wilson 1991;Pfaller et al 1991;Smythe and Newport 1991;Dasso et al 1992;Allan 1993;Holloway et al 1993;Newmeyer et al 1994;Hengartner 1995;King et al 1995King et al , 1996Yu et al 1996;Evans et al 1997;Kornbluth 1997;Thommes and Blow 1997;Lohka 1998;Pain et al 1998;de la Barre et al 1999;Desai et al 1999;Shirasu et al 1999;von Ahsen and Newmeyer 2000;Mandato et al 2001;Arias and Walter 2004). Since these cell-free extracts can be manipulated in many different ways, it has been possible to assess the role of specific proteins in distinct processes.…”

Section: Expression Screens In the Test Tubementioning

confidence: 99%

Xenomics

Amaya¹

2005

Genome Res.

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Xenopus genomics, or Xenomics for short, is coming of age. Indeed, biological insight into processes such as growth factor signaling and patterning of the early embryo is now being gained by combining the value of Xenopus as a model organism for cell and developmental biology with genomic approaches. In this review I address these recent advances and explore future possibilities gained from combining this powerful experimental system with genomic approaches, as well as how our quest to understand basic biological principles will be greatly facilitated though the marriage of Xenopus and genomics.Xenopus genomics is very much in its infancy. Although largescale sequencing efforts were slow to be initiated in this system, in the past 3-4 yr there has been an explosion of genomic information accumulating in Xenopus laevis and its diploid relative, Xenopus tropicalis (Fig.1). Since the beginning of 2003, >320,000 sequences have been deposited in public repositories for X. laevis and >1,100,000 for X. tropicalis, mostly in the form of expressed sequence tags (ESTs). With this expansive amount of new sequence information, X. tropicalis recently jumped into third place on the list of organisms with the most EST's, behind human and mouse. During the same period of time, the Joint Genome Institute (JGI) has been sequencing the X. tropicalis genome, using a shotgun approach, and it has recently completed ‫ן8‬ coverage. The JGI is currently in the process of assembling the X. tropicalis genome, and it is expected that the JGI will announce its results by the end of 2005. Now that such an extensive amount of genomic information is becoming available in Xenopus, how will this be useful in our scientific pursuits? This question is best answered by further asking, "What is the ultimate value of obtaining sequence information?" If the ultimate aim is not simply to catalog genes but to understand their function, then it would be very advantageous to find the ideal organisms to study gene function. It is here where the marriage of Xenopus and genomics will reap its full benefits, as Xenopus is perhaps the best vertebrate model organism for functional genomics. Functional screensXenopus is arguably the best available vertebrate model for systematic large-scale in vivo gene function analysis. In this section I review the different types of functional screens that can be performed in Xenopus.

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Section: Expression Screens In the Test Tubementioning

confidence: 99%

Xenomics

Amaya¹

2005

Genome Res.

View full text Add to dashboard Cite

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Analysis of Translational Activity of Extracts Derived from Oocytes and Eggs of Xenopus laevis

Cited by 1 publication

References 7 publications

Xenomics

Xenomics

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