2016
DOI: 10.1099/mic.0.000261
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Analysis of triacylglycerol accumulation under nitrogen deprivation in the red alga Cyanidioschyzon merolae

Abstract: Triacylglycerol (TAG) produced by microalgae is a potential source of biofuel. Although various metabolic pathways in TAG synthesis have been identified in land plants, the pathway of TAG synthesis in microalgae remains to be clarified. The unicellular rhodophyte Cyanidioschyzon merolae has unique properties as a producer of biofuel because of easy culture and feasibility of genetic engineering. Additionally, it is useful in the investigation of the pathway of TAG synthesis, because all of the nuclear, mitocho… Show more

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Cited by 19 publications
(14 citation statements)
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“…CMF187C was detected as a putative FAD4 protein catalyzing the synthesis of 16:1 (3- trans ) in plastid PG (Gao et al, 2009 ). We detected this unsaturated fatty acid (Sato and Moriyama, 2007 ; Toyoshima et al, 2016 ), but, unfortunately, we were not able to detect the signal of CMF187C within the cell by repeated attempts.…”
Section: Resultsmentioning
confidence: 87%
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“…CMF187C was detected as a putative FAD4 protein catalyzing the synthesis of 16:1 (3- trans ) in plastid PG (Gao et al, 2009 ). We detected this unsaturated fatty acid (Sato and Moriyama, 2007 ; Toyoshima et al, 2016 ), but, unfortunately, we were not able to detect the signal of CMF187C within the cell by repeated attempts.…”
Section: Resultsmentioning
confidence: 87%
“…Genes encoding PDAT, PDCT, PLC, and PLD were not detected in C. merolae (Table 1 , Supplementary Table 3 ). These results suggest that TAG is synthesized without the production of PC-derived DAG in C. merolae , although a large amount of TAG containing linoleic acid was detected in C. merolae by lipid analysis (Toyoshima et al, 2016 ). TAG could be produced by acylation of the DAG, which is formed from acyl CoA pool in equilibrium with the acyl groups in PC.…”
Section: Discussionmentioning
confidence: 97%
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“…C2 units: 7 for 14:0, 8 for 16:0, 9 for 18:0. a Calculated from data in ref. . b Specific radioactivity represents proportion of labeled carbon, 14 C/( 14 C + 12 C).…”
Section: Resultsmentioning
confidence: 99%
“…7C), suggesting that the acyltransferase in the coupled pathway might have a rather stringent substrate specificity. The high specific radioactivity (16.2 9 10 À6 , Table 5) and the high turnover rate of 14:0 (0.023 h À1 , Table 6) suggest that 14:0 is turned over rapidly, keeping a small pool of 14:0 in C. merolae (about 0.1-1%, [6]). It seems that newly synthesized 14:0 might be elongated to 16:0 by fatty acid elongation system in ER (Fig.…”
Section: Synthesis Of Acyl Groups In Red Algal Plastidsmentioning
confidence: 99%