Analysis of Triacylglycerols in Castor Oil Through Liquid Chromatography–Mass Spectrometry Based on Fourier Transform–Ion Cyclotron Resonance–Mass Spectrometry and Gas Chromatography–Mass Spectrometry

Du, Jialin; Gong, Chao; Pei, Xing-Li; Zhao, Haiyan; Xu, Xu

doi:10.1093/chromsci/bmy088

Cited by 4 publications

(3 citation statements)

References 25 publications

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“…We found that 0-OH-DAG was ∼4.27 mol% of the 2-OH-TAG ( Supplemental Table S1 ). Since 2-OH-TAG species make up ∼20% of the total oil ( Lin et al, 2003 ; Du et al, 2019 ), the 0-OH-DAG content made up ∼0.85 mol% of total oil, which is six times more than the 2-OH-DAG reported by Lin et al (2003) .…”

Section: Resultsmentioning

confidence: 90%

“…Although the highest activity was seen with ricinoleoyl-CoA and 2-OH-DAG, yielding 3-OH-TAG, which makes up 70%–80% of the TAG species in castor ( Lin et al, 2003 ; Du et al, 2019 ), the specificities for nonhydroxylated DAG and acyl-CoA species were substantial. Thus, our DGAT2 results with single acyl acceptors did not explain the very low levels of 0-OH-TAG and 1-OH-TAG seen in castor oil ( Lin et al, 2003 ).…”

Section: Resultsmentioning

confidence: 99%

“…Based on the biochemical properties of castor PDCT and DGAT2, analysis of DAG species in microsomal preparations from developing castor seeds, the distribution of ricinoleoyl groups in 2-OH-TAG in castor, and published composition of castor TAG species ( Lin et al, 2003 ; Du et al, 2019 ) a biosynthetic scheme for TAG synthesis in castor seeds emerges as depicted in Figure 10 .…”

Section: Discussionmentioning

confidence: 99%

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Phosphatidylcholine:diacylglycerol cholinephosphotransferase’s unique regulation of castor bean oil quality

et al. 2022

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Castor bean (Ricinus communis) seed oil (triacylglycerol, TAG) is composed of about 90% of the industrially important ricinoleoyl (12-hydroxy-9-octadecenoyl) groups. Here, phosphatidylcholine:diacylglycerol cholinephosphotransferase (PDCT) from castor bean was biochemically characterized and compared with camelina (Camelina sativa) PDCT. Diacylglycerols (DAGs) with ricinoleoyl groups were poorely used by Camelina, and their presence inhibited the utilization of DAG with ‘common’ acyl groups. In contrast, castor PDCT utilized DAG with ricinoleoyl groups similarly to DAG with common acyl groups and showed a 10-fold selectivity for DAG with one ricinoleoyl group over DAG with two ricinoleoyl groups. Castor diacylglycerol acyltransferase2 (DGAT2) specificities and selectivities towards different DAG and acyl-CoA species were assessed and shown to not acylate DAG without ricinoleoyl groups in the presence of ricinoleoyl-containing DAG. Eight-five percent of the DAG species in microsomal membranes prepared from developing castor endosperm lacked ricinoleoyl groups. Most of these species were predicted to be derived from PC, which had been formed by PDCT in exchange with DAG with one ricinoleoyl group. A scheme of the function of PDCT in castor endosperm is proposed where one ricinoleoyl group from de novo–synthesized DAG is selectivity transferred to PC. Non-ricinoleate DAG is formed and ricinoleoyl groups entering PC are re-used either in de novo synthesis of DAG with two ricinoleoyl groups or in direct synthesis of triricinoleoyl TAG by PDAT. The PC-derived DAG is not used in TAG synthesis but is proposed to serve as a substrate in membrane lipid biosynthesis during oil deposition.

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Section: Resultsmentioning

confidence: 90%