Analysis of triacylglycerols on porous graphitic carbon by high temperature liquid chromatography

Merelli, B.; Person, Marine de; Favetta, Patrick; Lafosse, M.

doi:10.1016/j.chroma.2007.05.005

Cited by 13 publications

(5 citation statements)

References 25 publications

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“…The TAG in the PGC column cannot be eluted by many typical reversed solvents, such as methanol, ACN, isopropanol, etc. The experimental results revealed that TAG retained in the PGC column can be washed out only by solvents with strong elution ability, such as toluene or chloroform, as previously reported (Merelli et al, ). However, chloroform presented a lower boiling point (61–62 °C) and a higher toxicity (LD50 of rat: 1194 mg kg −1 ), which troubled the laboratory.…”

Section: Resultssupporting

confidence: 83%

“…The resolution tended to deteriorate as the temperature increased and k decreased. A previous report (Merelli et al, ) demonstrated that the separation selectivity of TAG standards in a PGC column was changed by increasing temperature with a chloroform‐based mobile phase. However, the log k values of the four TAG generally decreased with increasing temperature (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…However, the use of PGC columns to characterize TAG from edible oils has not been published pervasively. Merelli, De Person, Favetta, and Lafosse () analyzed cocoa butter and shea butter using a PGC column with the CHCl 3 –isopropanol mobile phase. However, many peaks in the chromatogram are not well separated and recognized.…”

Section: Introductionmentioning

confidence: 99%

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Separation of Triacylglycerols from Edible Oil Using a Liquid Chromatography–Mass Spectrometry System with a Porous Graphitic Carbon Column and a Toluene–Isopropanol Gradient Mobile Phase

Zhang

Gong

Lü

et al. 2018

J Americ Oil Chem Soc

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This study presented a rapid and practical method of separating triacylglycerol (TAG) from edible oil using high-performance liquid chromatography (LC) coupled with atmospheric-pressure chemical ionization (APCI)/mass spectrometry (MS) system with a porous graphitic carbon column (150 mm × 2.1 mm, 5 μm) and a toluene-isopropanolformic acid mobile phase. After investigating the experimental conditions, the gradient toluene-isopropanol mobile phase containing 0.1% formic acid was changed from 50:50 to 80:20 in 30 min; the column temperature was set to 35 C, and APCI/MS was used in the positive-ion acquisition mode. The TAG retention displayed a special order and was summarized to fit as follows: S-ECN (special equivalent carbon number) = 2CN (carbon number) − 3dB (double bond number) -5uFA (unsaturated fatty-acid number). Then, the LC-MS method was applied to separate TAG in 6 vegetable oils, resulting in the recognition of 27 TAG in corn oil, 21 TAGs in olive oil, 22 TAG in sunflower seed oil, 28 TAG in soybean oil, 25 TAG in sesame oil, and 31 TAG in peanut oil. The TAG separation through the LC-MS method was rapid, reproducible, and durable.

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Section: Resultssupporting

confidence: 83%

Section: Resultsmentioning

confidence: 99%

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Separation of Triacylglycerols from Edible Oil Using a Liquid Chromatography–Mass Spectrometry System with a Porous Graphitic Carbon Column and a Toluene–Isopropanol Gradient Mobile Phase

Zhang

Gong

Lü

et al. 2018

J Americ Oil Chem Soc

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“…The ability of this graphitic material to retain small polar molecules is superior to that of silica-based sorbents (Gamelin et al, 1998;Fleming et al, 1993;Remaud et al, 2005;Ayrton et al, 1995;Gu and Lim, 1990;Cantu et al, 2001;Mazan et al, 2002;Gaudin et al, 2002;Jackson and Carr, 2002;Hanai, 2003;Antonio et al, 2007;Pereira et al, 2007;Merelli et al, 2007;Tachon et al, 2007;Xia et al, 2006;Roy et al, 2006;Bohnstedt et al, 2005;Reepmeyer et al, 2005;Xing et al, 2004;Thiebaut et al, 2006;Vial et al, 2001;Lepont et al, 2001;Holmgren et al, 2005). Moreover, porous graphitic carbon proved to be chemically and pH stable, and an inert stationary phase.…”

Section: Development Of a Pgc-hplc-ms/ms Methodsmentioning

confidence: 94%

“…Moreover, porous graphitic carbon proved to be chemically and pH stable, and an inert stationary phase. The following factors seem to infl uence the chromatographic behavior of PGC (Gaudin et al, 2002;Holmgren et al, 2005;Hanai, 2003;Jackson and Carr, 2002;Merelli et al, 2007;Vial et al, 2001;Thiebaut et al, 2006): (i) Dispersion forces and solvophobic eff ects due to the delocalization of the π-electrons in the graphite. This interaction causes PGC to behave as a reversed phase and predominantly important to retain non-polar compounds.…”

Section: Development Of a Pgc-hplc-ms/ms Methodsmentioning

confidence: 99%

A new, validated HPLC‐MS/MS method for the simultaneous determination of the anti‐cancer agent capecitabine and its metabolites: 5′‐deoxy‐5‐fluorocytidine, 5′‐deoxy‐5‐fluorouridine, 5‐fluorouracil and 5‐fluorodihydrouracil, in human plasma

Vainchtein

Rosing

Schellens

et al. 2009

Biomedical Chromatography

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A rapid and selective liquid chromatography/tandem mass spectrometric method was developed for the simultaneous determination of capecitabine and its metabolites 5'-deoxy-5-fluorocytidine (5'-DFCR), 5'-deoxy-5-fluorouracil (5'-DFUR), 5-fluorouracil (5-FU) and dihydro-5-fluorouracil (FUH(2)) in human plasma. A 200 microL human plasma aliquot was spiked with a mixture of internal standards fludarabine and 5-chlorouracil. A single-step protein precipitation method was employed using 10% (v/v) trichloroacetic acid in water to separate analytes from bio-matrices. Volumes of 20 microL of the supernatant were directly injected onto the HPLC system. Separation was achieved on a 30 x 2.1 mm Hypercarb (porous graphitic carbon) column using a gradient by mixing 10 mm ammonium acetate and acetonitrile-2-propanol-tetrahydrofuran (1 : 3 : 2.25, v/v/v). The detection was performed using a Finnigan TSQ Quantum Ultra equipped with the electrospray ion source operated in positive and negative mode. The assay quantifies a range from 10 to 1000 ng/mL for capecitabine, from 10 to 5000 ng/mL for 5'-DFCR and 5'-DFUR, and from 50 to 5000 ng/mL for 5-FU and FUH(2) using a plasma sample of 200 microL. Correlation coefficients (r(2)) of the calibration curves in human plasma were better than 0.99 for all compounds. At all concentration levels, deviations of measured concentrations from nominal concentration were between -4.41 and 3.65% with CV values less than 12.0% for capecitabine, between -7.00 and 6.59% with CV values less than 13.0 for 5'-DFUR, between -3.25 and 4.11% with CV values less than 9.34% for 5'-DFCR, between -5.54 and 5.91% with CV values less than 9.69% for 5-FU and between -4.26 and 6.86% with CV values less than 14.9% for FUH(2). The described method was successfully applied for the evaluation of the pharmacokinetic profile of capecitabine and its metabolites in plasma of treated cancer patients.

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Porous graphitic carbon: A versatile stationary phase for liquid chromatography

West

Elfakir

Lafosse

2010

Journal of Chromatography A

242

155

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Analysis of triacylglycerols on porous graphitic carbon by high temperature liquid chromatography

Cited by 13 publications

References 25 publications

Separation of Triacylglycerols from Edible Oil Using a Liquid Chromatography–Mass Spectrometry System with a Porous Graphitic Carbon Column and a Toluene–Isopropanol Gradient Mobile Phase

Separation of Triacylglycerols from Edible Oil Using a Liquid Chromatography–Mass Spectrometry System with a Porous Graphitic Carbon Column and a Toluene–Isopropanol Gradient Mobile Phase

A new, validated HPLC‐MS/MS method for the simultaneous determination of the anti‐cancer agent capecitabine and its metabolites: 5′‐deoxy‐5‐fluorocytidine, 5′‐deoxy‐5‐fluorouridine, 5‐fluorouracil and 5‐fluorodihydrouracil, in human plasma

Porous graphitic carbon: A versatile stationary phase for liquid chromatography

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