Analysis of tumor suppressor p53 status in head and neck squamous cell carcinoma

Šmardová, Jana; Ksicova, Katerina; Binková, Hana; Krpenský, Antonín; Pavlová, Šárka; Rottenberg, Jan; Koukalová, Hana

doi:10.3892/or.11.4.923

Cited by 8 publications

(7 citation statements)

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“…The p53 tumor suppressor gene is the most frequently mutated gene in human cancers, and in HNSCC p53 mutation has been demonstrated in ∼50% of cases . These mutations are thought to alter the function of p53, greatly impacting both carcinogenesis and treatment sensitivity .…”

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confidence: 99%

“…14 The p53 tumor suppressor gene is the most frequently mutated gene in human cancers, and in HNSCC p53 mutation has been demonstrated in 50% of cases. 15,16 These mutations are thought to alter the function of p53, greatly impacting both carcinogenesis and treatment sensitivity. 17,18 Furthermore, single nucleotide polymorphisms (SNPs) in the genes of p53 and its inhibitor MDM2 have been shown to be correlated with a poor therapeutic response and poor outcome in HNSCC patients.…”

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confidence: 99%

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Strong expression of survivin is associated with positive response to radiotherapy and improved overall survival in head and neck squamous cell carcinoma patients

Farnebo¹,

Tiefenböck²,

Ansell

et al. 2013

Int. J. Cancer

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Head and neck squamous cell carcinoma (HNSCC) is a malignancy that is associated with severe mortality despite advances in therapy. Today's standard treatment most commonly includes radiotherapy, often combined with chemotherapy or surgery. There are so far no established biomarkers to predict response to radiation, and thus the aim of this study was to investigate a series of markers that could potentially identify HNSCC patients who would benefit from radiotherapy. The selected markers, both proteins (epidermal growth factor receptor, survivin and p53), and single nucleotide polymorphisms (SNPs) in the genes of XRCC3, XRCC1, XPC, XPD, MDM2, p53 and FGFR4 were correlated to the response to radiotherapy and overall survival. Investigations were performed on pretreatment tumor biopsies from patients classified as responders or nonresponders to radiotherapy. Protein expression was examined using immunohistochemistry and the genotyping of specific SNPs was analyzed using PCR-RFLP or pyrosequencing. We found that survivin expression was significantly stronger in the responder group (p 5 0.003) and that patients with a strong survivin expression had a significantly better overall survival (p < 0.001). Moreover, downregulation of survivin by siRNA in two HNSCC cell lines significantly decreased their sensitivity to radiation. Among the SNPs analyzed, patients with the XPD Lys751Gln SNP had a significantly shorter overall survival (p 5 0.048), and patients with the FGFR4 Gly388Arg SNP had a significantly longer overall survival (p 5 0.010). In conclusion, our results suggest that survivin plays an important role in the response to radiotherapy and may be a useful marker for predicting radiotherapy response in patients with HNSCC.

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confidence: 99%

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confidence: 99%

Strong expression of survivin is associated with positive response to radiotherapy and improved overall survival in head and neck squamous cell carcinoma patients

Farnebo¹,

Tiefenböck²,

Ansell

et al. 2013

Int. J. Cancer

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“…With FASAY, the number of red yeast colonies transformed with TP53 amplicons, which may reflect the quality of RNA preservation (21,23,24), was assessed on the same extracted mRNA samples. For uvula specimens, the paired samples t-test, performed on normalized densitometric values of TP53 amplicons, unambiguously confirmed significant differences between the two grinding methods (p-0.001).…”

Section: Discussionmentioning

confidence: 99%

“…This assay was performed with mRNA extracted from both uvula and oral carcinoma samples, as previously described (21)(22)(23)(24). Briefly, the transcriptional activity of human p53 in cells was assessed in Saccharomyces cerevisiae, where it activates a p53 target gene (Ade2).…”

Section: Functional Analysis Of Separated Alleles In Yeast (Fasay)mentioning

confidence: 99%

Automated cell disruption is a reliable and effective method of isolating RNA from fresh snap-frozen normal and malignant oral mucosa samples

Vorst

Dekairelle²,

Irenge³

et al. 2009

Clinical Chemistry and Laboratory Medicine

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“…We have recently shown that the temperature sensitivity of p53 mutants can also be tissue specific (Pavlova et al, 2003; Smardova et al, 2004). Routinely, p53 status has been assessed by immunohistochemical methods or by DNA analysis, and these methods do not recognize temperature sensitivity of p53 mutants.…”

Section: Tissue Specificity Of Certain Functional Types Of P53 Mutantsmentioning

confidence: 99%

Functional analysis of p53 tumor suppressor in yeast

2005

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Analysis of tumor suppressor p53 status in head and neck squamous cell carcinoma

Cited by 8 publications

References 0 publications

Strong expression of survivin is associated with positive response to radiotherapy and improved overall survival in head and neck squamous cell carcinoma patients

Strong expression of survivin is associated with positive response to radiotherapy and improved overall survival in head and neck squamous cell carcinoma patients

Automated cell disruption is a reliable and effective method of isolating RNA from fresh snap-frozen normal and malignant oral mucosa samples

Functional analysis of p53 tumor suppressor in yeast

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