Analysis of underivatized C12–C18 fatty acids by reversed-phase ion-pair high-performance liquid chromatography with conductivty detection

Tsuyama, Yoshiko; Uchida, Toshiro; Goto, Takeshi

doi:10.1016/0021-9673(92)85005-e

Cited by 24 publications

(10 citation statements)

References 29 publications

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“…although their poor ultraviolet absorbance makes the direct quantitation by UV detection in real samples difficult. Alternatives have been described such as refractive index detection [4], conductivity detection [5] and post-column derivatization for UV-vis absorption detection [6] or chemiluminescence detection [7]. Nevertheless, the usual liquid chromatographic separation of fatty acids includes the pre-column formation of UV-absorbing derivatives such as phenacyl esters [8], naphthacyl esters [9], 2-nitrophenylhydrazides [10], and several others [11,12].…”

mentioning

confidence: 99%

Determination of free fatty acids by capillary zone electrophoresis

Buchberger

Winna

1996

Mikrochim Acta

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Abstract. Capillary zone electrophoresis was investigated for the separation of free fatty acids as an alternative to well established techniques such as GC or HPLC. The analysis was performed with indirect UV detection in a counterelectroosmotic flow mode using a diethylbarbiturate carrier electrolyte at a pH between 10 and 11 in a mixed aqueous-organic solvent. Separation of saturated and unsaturated fatty acids could be achieved after bromination of the double bonds. Problems with wall adsorption of fatty acids could be overcome by increasing the temperature and using a high concentration of a zwitterionic reagent to inactivate the silica surface. Increased sensitivity could be achieved after preconcentration on alumina. The method was applied to the determination of free fatty acids in dairy products. The advantages compared to traditional methods include short analysis times and simple preparation steps.

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mentioning

confidence: 99%

Determination of free fatty acids by capillary zone electrophoresis

Buchberger

Winna

1996

Mikrochim Acta

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“…HPLC is the most commonly employed tool for the analysis of the pharmaceutical products but the method is not successful in the quantitative analysis of fatty acids 17 . Most of these techniques use derivatization reagents.…”

Section: Introductionmentioning

confidence: 99%

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2017

IJPSR

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Present paper reports the chemical constituents in the fatty acids obtained from the whole plant of diploid and tetraploid Physalis angulata Linn. collected from hot desert areas of Rajasthan. The fatty acid analysis was carried out by gas chromatography-flame ionization detector (GC-FID). Meiotic studies confirm the ploidy level of the plant. Diploid (n = 12) cytotype is reported from Udaipur district and tetraploid (n = 24) cytotype from Sri Ganganagar. There is a considerable difference in the fatty acid constituents in the cytotypes. Major constituents of fatty acids are Oleic acid (24.93%, 2x; 18.71%, 4x); capric acid (13.4%, 2x; 0.14%, 4x); Palmitic acid (6.966%, 2x; 12.38%, 4x); Linoleic acid (5.5%, 2x; 9.76%, 4x) and Linolenic acid (6%, 2x; 11.67%, 4x). The main objective of the object work was to compare the fatty acid composition within two cytotypes. Such analysis of plants is very important on commercial level for the productions of cosmetic and has great interest in pharmaceutical companies for the production of drugs.

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“…Ces méthodes ont été proposées non seulement en chromatographie en phase gazeuse, grâce à l'utilisation de colonnes spécialement conçues pour ces applications [11,12], mais également en chromatographie en phase supercritique (SFC) [13] ou bien encore en chromatographie liquide en association avec une détection UV à basse longueur d'onde [14]. L'isotachophorèse a été égale-ment largement utilisée [15,16].…”

Section: Introductionunclassified

L'analyse des acides gras en électrophorèse capillaire

Mofaddel¹,

Desbènes-Monvernay²

1999

Analusis

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Analysis of underivatized C12–C18 fatty acids by reversed-phase ion-pair high-performance liquid chromatography with conductivty detection

Cited by 24 publications

References 29 publications

Determination of free fatty acids by capillary zone electrophoresis

Determination of free fatty acids by capillary zone electrophoresis

Untitled

L'analyse des acides gras en électrophorèse capillaire

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