Analysis of underivatized gentamicin by capillary electrophoresis with UV detection

Curiel, Harold; Vanderaerden, W.; Vélez, Hermán; Hoogmartens, Jos; Schepdael, Ann Van

doi:10.1016/j.jpba.2007.01.029

Cited by 32 publications

(15 citation statements)

References 22 publications

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“…Due to these regulations and the potential dangers of gentamicin residues remaining in foods, it is necessary to have a detection method that is capable of detecting such low concentrations of the JFDS-2017-1753Submitted 10/25/2017, Accepted 4/9/2018 antibiotic. Existing methods for gentamicin detection include immunoassays (Chen, Li, He, Tang, & Xiao, 2010), HPLC (Kuehl, 2012), and capillary electrophoresis (Curiel, Vanderaerden, Velez, Hoogmartens, & Van Schepdael, 2007). These methods tend to be labor intensive, require expensive equipment, or do not have a sufficiently low limit of detection.…”

Section: Introductionmentioning

confidence: 99%

Cysteamine‐Modified Gold Nanoparticles as a Colorimetric Sensor for the Rapid Detection of Gentamicin

Gukowsky

Tan

Han

et al. 2018

Journal of Food Science

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This study demonstrates that cysteamine-modified gold nanoparticles could be used as a rapid and efficient tool for gentamicin detection. This technique is cheaper, simpler, and more effective than many other methods that are currently used for detecting the antibiotic in industrial and commercial applications. It has a great potential to be practically applied as a rapid screening method for gentamicin and gentamicin-like compounds in food and environmental samples.

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Section: Introductionmentioning

confidence: 99%

Cysteamine‐Modified Gold Nanoparticles as a Colorimetric Sensor for the Rapid Detection of Gentamicin

Gukowsky

Tan

Han

et al. 2018

Journal of Food Science

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“…The separation and detection of amikacin and urea have been a great challenge, due to the lack of UV absorbing properties. Various methods have been applied for determination of aminoglycosides in various matrices, including microbiological assay [3], radioenzymatic assay [4], immunoassays [5], liquid chromatography (LC) [6][7][8][9] and capillary electrophoresis (CE) [10][11][12][13][14][15][16][17][18]. Several of these methods used precolumn or postcolumn derivatization with either UV or fluorescence detection [6,14,15].…”

Section: Introductionmentioning

confidence: 99%

“…Among these, in combination with LC, pulsed electrochemical detection (PED) is advisable [7][8][9]. Combined with CE, borate complexation [17], indirect UV detection [12], amperometric detection [18] and UV detection, performed in Tris buffer at pH 9.1 with high concentration of sodium pentanesulfonate (SPS) [10] or in pyrophosphate buffer [11] have been described for the analysis of aminoglycosides. However, these CE methods are neither very sensitive nor selective.…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of a Chromatographic and Electrophoretic Method for the Determination of Amikacin and Urea in Bronchial Epithelial Lining Fluid

et al. 2012

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Methods were developed to determine amikacin and urea in bronchial epithelial lining fluid of neonates. Urea was determined as ammonium by capillary electrophoresis in combination with capacitively coupled contactless conductivity detection (CE-C 4 D). The ammonium was produced by enzymatic conversion of urea with urease enzyme. The background electrolyte (BGE) contained 30 mM malic acid, adjusted to pH 4.1 by L-arginine, and 10 mM 18-Crown-6. Lithium was used as internal standard. A ?30 kV was applied on a fused silica capillary with 75 lm internal diameter (ID) and total length of 65 cm (41 cm to C 4 D detector). The optimized separation was obtained in \3 min with good linearity (R 2 = 0.9998) for urea concentrations ranging from 0.6 to 24 mg L -1 . It also shows a good repeatability expressed by the RSD which is 0.7 and 1.3 % for intraday and interday precision, respectively. The LOD and LOQ are 0.14 and 0.5 mg L -1 respectively. As the CE-C 4 D method was not sensitive enough for amikacin, the latter was determined using liquid chromatography combined with pulsed electrochemical detection (LC-PED). The LOQ for amikacin base was found to be 0.06 mg L -1 . In addition, the linearity was good (R 2 [ 0.995) as well as the repeatability (RSD = 0.1 %, n = 3). For both the CE and LC method, no interference of matrix components was observed and the recoveries were found to be close to 100 %.

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“…The indirect methods required pre- or post-column derivatization with either UV [10–13] or fluorescence detection [14–17] or CE [18, 19], were time consuming, tedious and not accurate enough. Direct methods included detection techniques such as refractive index (RI) [20], ELSD [21, 22], CAD [23], CE [24], ED [25, 26] or MS [27–29]. The advantages and disadvantages of these methods were discussed in [7, 23, 30].…”

Section: Introductionmentioning

confidence: 99%

Determination of Gentamicin Sulphate Composition and Related Substances in Pharmaceutical Preparations by LC with Charged Aerosol Detection

Stypulkowska

Błażewicz

Fijałek

et al. 2010

Chroma

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A new, simple and repeatable liquid chromatography method with charged aerosol detection (LC-CAD) for the determination of gentamicin sulphate composition and related substances has been developed. Gentamicin lacks of chromophores, therefore its determination is quite problematic. Using a universal CAD enables to achieve good separation without sample derivatization. Mass spectrometry was employed to confirm the LC-CAD peak profile. The proposed method was validated and applied for the determination of gentamicin sulphate composition and related substances in pharmaceutical preparations.

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Analysis of underivatized gentamicin by capillary electrophoresis with UV detection

Cited by 32 publications

References 22 publications

Cysteamine‐Modified Gold Nanoparticles as a Colorimetric Sensor for the Rapid Detection of Gentamicin

Cysteamine‐Modified Gold Nanoparticles as a Colorimetric Sensor for the Rapid Detection of Gentamicin

Development and Validation of a Chromatographic and Electrophoretic Method for the Determination of Amikacin and Urea in Bronchial Epithelial Lining Fluid

Determination of Gentamicin Sulphate Composition and Related Substances in Pharmaceutical Preparations by LC with Charged Aerosol Detection

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