Analysis of urinary metabolites for metabolomic study by pressurized CEC

Xie, Guoxiang; Su, Mingming; Peng, Li; Gu, Xue; Yan, Chao; Qiu, Yunping; Li, Houkai; Wang, Jia

doi:10.1002/elps.200700420

Cited by 14 publications

(9 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…leukaemia [7], lymphoma [8], nasopharyngeal cancer, breast cancer [9–11], colorectal cancer [12, 13], bronchogenic carcinoma [14], bladder cancer [15–18] and hepatocellular carcinoma [6]. The fact that nucleoside levels do not depend on age and sex [5] suggests that these variables do not need to be taken into account when developing relevant diagnostic tests.…”

Section: Introductionmentioning

confidence: 99%

The state-of-the-art determination of urinary nucleosides using chromatographic techniques “hyphenated” with advanced bioinformatic methods

et al. 2011

View full text Add to dashboard Cite

Over the last decade metabolomics has gained increasing popularity and significance in life sciences. Together with genomics, transcriptomics and proteomics, metabolomics provides additional information on specific reactions occurring in humans, allowing us to understand some of the metabolic pathways in pathological processes. Abnormal levels of such metabolites as nucleosides in the urine of cancer patients (abnormal in relation to the levels observed in healthy volunteers) seem to be an original potential diagnostic marker of carcinogenesis. However, the expectations regarding the diagnostic value of nucleosides may only be justified once an appropriate analytical procedure has been applied for their determination. The achievement of good specificity, sensitivity and reproducibility of the analysis depends on the right choice of the phases (e.g. sample pretreatment procedure), the analytical technique and the bioinformatic approach. Improving the techniques and methods applied implies greater interest in exploration of reliable diagnostic markers. This review covers the last 11 years of determination of urinary nucleosides conducted with the use of high-performance liquid chromatography in conjunction with various types of detection, sample pretreatment methods as well as bioinformatic data processing procedures.Electronic supplementary materialThe online version of this article (doi:10.1007/s00216-011-4789-6) contains supplementary material, which is available to authorized users.

show abstract

Section: Introductionmentioning

confidence: 99%

The state-of-the-art determination of urinary nucleosides using chromatographic techniques “hyphenated” with advanced bioinformatic methods

et al. 2011

View full text Add to dashboard Cite

show abstract

“…According to the authors method can be used as a de facto standard for metabolome analysis. Only one paper has used pressurized capillary electrochromatography CEC (pCEC) with gradient elution for the metabolomic study of urinary samples [40] as an alternative chromatographic separation tool with higher degree of resolution, selectivity, sensitivity, and efficiency. The pCEC separation of urinary samples was performed on a RP column packed with C18, 5 m particles with an ACN/water mobile phase containing TFA.…”

Section: Selected Methodsmentioning

confidence: 99%

Capillary electrophoresis as a metabolomics tool for non-targeted fingerprinting of biological samples

Barbas

Moraes

Villaseñor

2011

Journal of Pharmaceutical and Biomedical Analysis

View full text Add to dashboard Cite

Metabolomics, understood as a data driven strategy trying to find markers of a situation under study without a priori hypothesis, has rapidly caught the attention and evolved from the simple pattern recognition strategy, which was a great innovation at its origins, to the interest for the final identification of markers responsible for class separation, i.e., from data to knowledge. Due to differences in physico-chemical properties and concentrations of the metabolites, but also due to differences in matrix properties, cross-platform approaches are proving to increase the capability of information. Once more techniques do not compete. This is the scene where capillary electrophoresis (CE) has its niche to provide information mainly on polar or ionic compounds in biological fluids. General advantages and disadvantages of CE for sample fingerprinting will be discussed and methods will be classified depending on the detection system (UV or MS) as this strongly affects all the conditions. Recent developments will be presented in different biological fluids, although urine is without a doubt the preferred sample for CE analysis.

show abstract

“…Although an improved separation and extra selectivity can be obtained using MEKC methods, the coupling of MEKC to MS is problematic and often provides limited sensitivity. Recently, the potential of pressure‐assisted CEC, an analytical separation technique that combines the high peak efficiency of CE with the stationary phase selectivity of LC, has been evaluated for metabolic profiling of rat urine 22. Figure 1 clearly shows that CEC is able to measure more urinary metabolites with good resolution than a standard RPLC or capillary LC method.…”

Section: Ce‐ms Methodologymentioning

confidence: 99%

CE‐MS in metabolomics

2009

View full text Add to dashboard Cite

An overview of the use of CE-MS in the field of metabolomics is provided. Metabolomics is concerned with the comprehensive analysis of endogenous low-molecular-weight compounds in biological samples. CE-MS has demonstrated to be a powerful technique for the profiling of polar metabolites in biological samples. This review covers the use of various CE separation modes, capillary coatings, MS analyzers, sample preparation techniques, and data analysis methods used in CE-MS for metabolomics. The applicability of CE-MS in metabolomics research is illustrated by giving examples of the analysis of bacterial extracts, plant extracts, urine, plasma, and cerebrospinal fluid samples. The relevant CE-MS metabolomics studies published between 2000 and 2008 are presented in tabular form, including information on sample type and pretreatment and MS detection mode. Future developments with regard to the use of alternative ionization techniques, the use of coupled separation systems and the potential of microchip CE systems for metabolomics are discussed.

show abstract

Analysis of urinary metabolites for metabolomic study by pressurized CEC

Cited by 14 publications

References 35 publications

The state-of-the-art determination of urinary nucleosides using chromatographic techniques “hyphenated” with advanced bioinformatic methods

The state-of-the-art determination of urinary nucleosides using chromatographic techniques “hyphenated” with advanced bioinformatic methods

Capillary electrophoresis as a metabolomics tool for non-targeted fingerprinting of biological samples

CE‐MS in metabolomics

Contact Info

Product

Resources

About