Analysis of volatile aldehyde biomarkers in human blood by derivatization and dispersive liquid–liquid microextraction based on solidification of floating organic droplet method by high performance liquid chromatography

Lv, Lili; Xu, Hui; Song, Dandan; Cui, Yanfang; Hu, Sheng; Zhang, Ganbing

doi:10.1016/j.chroma.2010.01.081

Cited by 109 publications

(33 citation statements)

References 44 publications

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“…The recovery of the proposed method was determined by using serum samples spiked with standard mixture of aldehydes at the three concentrations (5, 10 and 15 nM) before protein precipitation. Recoveries were expressed as [(found amount/ spiked amount) x100] and found to be more than 88%, which is higher than previous methods [13,14] and the results are listed in Table 3.…”

Section: Determination Of Aliphatic Aldehydes In Human Serummentioning

confidence: 76%

“…Medium chain length aliphatic aldehydes are produced as degradation end products of lipid peroxidation process in vivo [8] and their levels have been measured in different biological fluids [1,[9][10][11][12][13][14][15][16][17][18][19][20]. The presence of these aldehydes has been related to various pathological conditions such as carcinogenesis, atherosclerosis, diabetes mellitus and rheumatic arthritis [10,12,18,21].…”

Section: Introductionmentioning

confidence: 99%

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Chromatographic determination of aliphatic aldehydes in human serum after pre-column derivatization using 2,2′-furil, a novel fluorogenic reagent

Ali

Kishikawa

Ohyama

et al. 2013

Journal of Chromatography A

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A novel, highly sensitive and selective fluorimetric liquid chromatographic method for simultaneous determination of medium chain aliphatic aldehydes was developed. The method was based on the derivatization of aliphatic aldehydes with 1,2-di(2-furyl)-1,2-ethanedione (2,2`-furil), a novel fluorogenic reagent, to form highly fluorescent difurylimidazole derivatives. The fluorescence derivatives were separated in less than 20 min on a reversed-phase ODS column using an isocratic elution with a mixture of methanol-water (80:20, v/v%). The detection limits were from 0.19 to 0.50 nM (1-10 fmol/injection) at a signal-tonoise ratio (S/N) of 3. This method was successfully applied for monitoring of aliphatic aldehydes in healthy human sera by a simple pretreatment procedure without interferences from serum constituents.

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Section: Determination Of Aliphatic Aldehydes In Human Serummentioning

confidence: 76%

Section: Introductionmentioning

confidence: 99%

Chromatographic determination of aliphatic aldehydes in human serum after pre-column derivatization using 2,2′-furil, a novel fluorogenic reagent

Ali

Kishikawa

Ohyama

et al. 2013

Journal of Chromatography A

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“…Lili et al [106] develop the analysis of volatile aldehyde biomarkers in human blood by derivatization and DLLME-SFO. In the derivatization and extraction procedure, 2,4-dinitrophenylhydrazine as derivatization reagent and formic acid as catalyzer were injected into the sample solution for derivatization with aldehydes, then the formed hydrazones were rapidly extracted by DLLME with 1-dodecanol as extraction solvent.…”

Section: Liquid Chromatographymentioning

confidence: 99%

Separation and Preconcentration by Dispersive Liquid–Liquid Microextraction Procedure: Recent Applications

Ojeda

Rojas

2011

Chromatographia

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“…In CPE, a green pretreatment method, surfactants are used instead of organic solvents. Studies show that use of DLLME, DLLME-SFO and CPE for extracting various analytes in water samples provides high enrichment factors [24][25][26][27][28][29].…”

Section: Introductionmentioning

confidence: 99%

Micro-scale quantitation of ten phthalate esters in water samples and cosmetics using capillary liquid chromatography coupled to UV detection: effective strategies to reduce the production of organic waste

Feng

Jiang

2012

Microchim Acta

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We have extracted ten phthalate esters (C1 to C8) using six different micro-scale methods for extraction, and then separated them by capillary liquid chromatography coupled to UV detection. The methods included liquid-liquid extraction, ultrasonic-assisted extraction, microwave-assisted extraction, dispersive liquid-liquidmicroextraction, dispersive liquid-liquid microextraction solidification of floating organic droplets, and cloud point extraction. The linear range of the analytes is from 0.5 to 50 μg mL −1 , and the detection limits range from 0.02 to~0.17 μg mL −1 . The precision and accuracy of all intra-and inter-day analyses are <5.5%. We find that dispersive liquid-liquid microextraction solidification of floating organic droplet (DLLME-SFO) is the best method for quantification of most phthalate esters in water samples and cosmetics because of its low limit of detection and high extraction efficiencies.

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Analysis of volatile aldehyde biomarkers in human blood by derivatization and dispersive liquid–liquid microextraction based on solidification of floating organic droplet method by high performance liquid chromatography

Cited by 109 publications

References 44 publications

Chromatographic determination of aliphatic aldehydes in human serum after pre-column derivatization using 2,2′-furil, a novel fluorogenic reagent

Chromatographic determination of aliphatic aldehydes in human serum after pre-column derivatization using 2,2′-furil, a novel fluorogenic reagent

Separation and Preconcentration by Dispersive Liquid–Liquid Microextraction Procedure: Recent Applications

Micro-scale quantitation of ten phthalate esters in water samples and cosmetics using capillary liquid chromatography coupled to UV detection: effective strategies to reduce the production of organic waste

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