Analysis of Volatile Constituents in Curcuma Species, viz. C. aeruginosa, C. zedoaria, and C. longa, from Nepal

Beverage mixtures based on pineapple (Ananas comosus) and turmeric (Curcuma longa) juice as a ready-to-drink product were developed, and their physicochemical, nutritional, and sensory properties were evaluated. Four different concentrations of turmeric juice (5%, 10%, 15%, and 20% (v/v)) were added to pineapple juice to make turmeric-fortified pineapple (TFP) juice samples. Pineapple juice without turmeric was the control. The L*, a*, b*, titratable acidity (TA), total antioxidant capacity, and %DPPH scavenging values, as well as the concentrations of the phenolic compounds curcumin and demethoxycurcumin, were significantly increased with increasing turmeric concentration. Thirty volatile compounds were detected in the mixed juice samples with turmeric. Most of the turmeric-specific compounds, including monoterpenes, sesquiterpenes and turmerones, were detected in the TFP juice samples. While the antioxidant activity of the juice samples increased with increasing turmeric concentration, the pineapple juice fortified with 10% turmeric (10%T) had the best overall quality as determined by panelists. Greater concentrations of turmeric were associated with decreased palatability due to reduced mouthfeel and sweetness and increased aftertaste and sourness. These results suggest that the 10%T juice could be developed into a commercial functional beverage with increased overall flavor and nutritional quality.

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“…Those compounds significantly (p < 0.05) affect the aftertaste of the juices. A comparable trend was found in a previous study [30].…”

Section: Sensory Evaluationsupporting

confidence: 92%

Physical, Chemical, and Sensory Properties of a Turmeric-Fortified Pineapple Juice Beverage

Follett

Wall

Duff

et al. 2023

Foods

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“…37 Chiral GC–MS was carried out as previously described. 38 The enantiomers were identified by comparing retention times and mass spectral fragmentation patterns with authentic samples acquired from Sigma-Aldrich, and the enantiomeric ratios were calculated from the peak areas.…”

Section: Methodsmentioning

confidence: 99%

Chemical-Enantiomeric Characterization and In-Vitro Biological Evaluation of the Essential Oils from Elsholtzia strobilifera (Benth.) Benth. and E. blanda (Benth.) Benth. from Nepal

Paudel,

Satyal,

Setzer

et al. 2023

Natural Product Communications

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Background: Essential oils (EOs) are one of the most important groups of plant secondary metabolites responsible for their various biological activities. They also have wide applications as natural ingredients in many fields like the food, cosmetic, and medicine industries. This study was conducted to determine the chemical composition, enantiomeric distribution, and in-vitro biological activities of Elsholtzia strobilifera (Benth.) Benth. and E. blanda (Benth.) Benth. essential oils of Lamiaceae from Nepal. Methods: Essential oils obtained from hydro-distillation were analyzed by gas-chromatography mass-spectrometry (GC–MS) for their chemical constituents and the enantiomeric distribution of chiral terpenoids were determined by chiral GC–MS. Microbroth dilution was used to investigate the in-vitro antimicrobial activity, while cytotoxic activities were determined by cell viability using a Cell Counting Kit assay. The DPPH (2,2-diphenyl-1-picrylhydrazyl) radical-scavenging and FRAP (ferric-reducing antioxidant power) assays were used to evaluate the antioxidant activities. Results: A total of 55 compounds were identified in E. strobilifera with pinocarvone (40.7%), β-pinene (10%) and cis-pinocamphone (7.9%) as predominant constituents. While, 41 compounds were identified in the E. blanda EOs, including dihydrotagetone (49.1%), ( Z)-tagetone (15.6%) and ( E)-tagetone (8%) as major components; both essential oils were dominated by oxygenated monoterpenoids. The chiral separation and analyses of 12, E. strobilifera and 7, E. blanda essential oil components were performed, respectively, where (–)-β-caryophyllene and (–)-germacrene D were detected as enantiomerically pure in both oils. Elsholtzia EOs exhibited strong antifungal activity with MIC value of 156.3 µg/mL against Aspergillus niger, whereas, E. strobilifera EO against Staphylococcus epidermidis and E. blanda EO against Staphylococcus aureus showed inhibitory effects with MIC values of 156.3 µg/mL. E. strobilifera EO showed very active in-vitro cytotoxicity against the NIH-3T3 cell line with IC50 of 20.2 µg/mL, whereas E. blanda EO displayed effective cytotoxicity against MCF-7 cell line with IC50 of 33.1 µg/mL. The antioxidant activity of E. strobilifera EO was relatively good with IC50 of 188.8 ± 0.7 µg/mL in DPPH and EC50 value of 348.0 ± 0.1 µg/mL in FRAP, as compared to that of E. blanda EO. Conclusion: Elsholtzia EOs showed prominent biological activities emphasizing their importance as natural ingredients.

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“…A Shimadzu GC-MS-QP2010S with EI mode (70 eV) and B-Dex 325 chiral capillary GC column was used to perform the enantiomeric analysis of Cymbopogen species (C. winterianus, C. citratus, and C. martini) Eos, as previously described [55]. A comparison of retention times and mass spectral fragmentation patterns with authentic samples obtained from Sigma-Aldrich (Milwaukee, WI, USA) was used to identify the enantiomers.…”

Section: Enantiomeric Analysis By Chiral Gas Chromatography-mass Spec...mentioning

confidence: 99%

Essential Oil Composition Analysis of Cymbopogon Species from Eastern Nepal by GC-MS and Chiral GC-MS, and Antimicrobial Activity of Some Major Compounds

Dangol¹,

Poudel²,

Ojha³

et al. 2023

Molecules

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Cymbopogon species essential oil (EO) carries significant importance in pharmaceuticals, aromatherapy, food, etc. The chemical compositions of Cymbopogon spp. Viz. Cymbopogon winterianus (citronella) Cymbopogon citratus (lemongrass), and Cymbopogon martini (palmarosa) were analyzed by gas chromatography–mass spectrometry (GC-MS), enantiomeric distribution by chiral GC-MS, and antimicrobial activities of some selected pure major compound and root and leaves EOs of citronella. The EO of leaves of Cymbopogon spp. showed comparatively higher yield than roots or other parts. Contrary to citral (neral and geranial) being a predominant compound of Cymbopogon spp., α-elemol (53.1%), α-elemol (29.5%), geraniol (37.1%), and citral (90.4%) were detected as major compounds of the root, root hair with stalk, leaf, and root stalk with shoot of citronella EO, respectively. Palmarosa leaves’ EO contains neral (36.1%) and geranial (53.1) as the major compounds. In the roots of palmarosa EO, the prime components were α-elemol (31.5%), geranial (25.0%), and neral (16.6%). Similarly, lemongrass leaves’ EO contains geraniol (76.6%) and geranyl acetate (15.2%) as major compounds, while the root EO contains a higher amount of geraniol (87.9%) and lower amount of geranyl acetate (4.4%). This study reports for the first time chiral terpenoids from Cymbopogon spp. EOs. Chiral GC-MS gave specific enantiomeric distributions of nine, six, and five chiral terpenoids in the root, root stalk with a shoot, and leaves of citronella EOs, respectively. Likewise, four and three chiral terpenoids in the root and leaves of lemongrass oil followed by two chiral terpenoids in the leaves and root of palmarosa EOs each. Additionally, the root and leaves’ EOs of citronella exhibit noticeable activity on bacteria such as Staphylococcus aureus, Staphylococcus epidermidis, and Streptococcus pyogenes and fungus such as Candida albicans, Microsporum canis, and Trichophyton mentagrophytes. So, geranial-, neral-, geraniol-, and citronellal-rich EOs can be used as an alternative antimicrobial agent.

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Analysis of Volatile Constituents in Curcuma Species, viz. C. aeruginosa, C. zedoaria, and C. longa, from Nepal

Cited by 19 publications

References 36 publications

Physical, Chemical, and Sensory Properties of a Turmeric-Fortified Pineapple Juice Beverage

Physical, Chemical, and Sensory Properties of a Turmeric-Fortified Pineapple Juice Beverage

Chemical-Enantiomeric Characterization and In-Vitro Biological Evaluation of the Essential Oils from Elsholtzia strobilifera (Benth.) Benth. and E. blanda (Benth.) Benth. from Nepal

Essential Oil Composition Analysis of Cymbopogon Species from Eastern Nepal by GC-MS and Chiral GC-MS, and Antimicrobial Activity of Some Major Compounds

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