Analysis of Whole Cell Protein Profiles by SDS-PAGE to Identify Indigenous Cellulose-producer Acetic Acid Bacteria

Abstract

This study was carried out to analyze the suitability of the identification of four indigenous cellulose producing acetic acid bacterial isolates (ANG29, KRE65, ANG32 and SAL53) based on the analysis of whole cellular protein profiles against identification based on phenotypic traits. Whole cellular protein profiles were determined by sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDSPAGE) method. The whole cellular protein profiles obtained from sample isolates, were compared with reference isolates for species identification. The results showed that based on visual observations can be determined as much as 12 bands of protein with a molecular weight of 19,099 KDa up to 132.182 KDa. Based on the analysis of protein bands were detected visually, fourth indigenous cellulose producing acetic acid bacterial isolates in the study had a higher similarity profile to the reference strain Gluconacetobacter xylinus BTCC 769 compared with other reference strains namely G. hansenii NBRC 14820 T . This condition is consistent with the results of the identification of fourth cellulose producing acetic acid bacterial isolates based on phenotypic traits. Thus, the whole cellular protein profiles by SDSPAGE technique can be used as a one of method to identification of cellulose producing acetic acid bacterial isolates.

Polyphasic Identification of Amylolytic Bacteria Producing Bioplastic Poly-β-hydroxybutyrate (PHB)

“…High resolution polyacrylamide gel electrophoresis (PAGE) of proteins with computerized analysis of profiles provided an effective approach to the investigation of taxonomic relationships among many bacterial species (Berber 2004;Berber & Yenidunya 2005). Several investigators have used SDS-PAGE of soluble bacterial polypeptides data for numerical taxonomy and an effective approach to the investigation of taxonomic relationships among many bacterial species (Berber 2004;Berber & Yenidunya 2005;Ghazi et al 2009;Santos et al 2012;Sarkono et al 2016). Therefore, this study aimed to identify the three isolates of bacterial producing bioplastics from Southeast Sulawesi, Indonesia by polyphasic taxonomy approach, particularly based on the characters of phenotypic, whole cell protein profile and genotypic characters, so as to illustrate the similarities between bacterial and the identity of the local bacterial isolates can be unveiled.…”