Analysis of Wound Healing Activity from Rose Petal Extract Ointment in Wistar Rats.

Abstract

Basic Health Report (2013) reports that burns rank sixth in unintentional injuries. Rose petals have various pharmacological effects that can increase wound healing activity. In this study, the activity of healing burns using rose petals as an ointment was tested on 25 male wistar rats which were grouped into: control (Ointment base), standard (Nebacetin), 7.5%, 10%, and 15% of rose petal ointment doses, respectively. The Rose petal ointment was made from the extraction by maceration. Two parameters used for evaluation were wound contraction and epithelialization period. In addition, the skin was also excised for histological studies. The highest wound contraction was obtained in the standard group (87.47%), followed by 15% (85.07%), 10% (73.33%), 7.5% (69.16%) rose petal ointment, control group (64.27%). While the length of epithelialization of each treatment group (P-value = 0.301) did not show a significant difference in all groups. Histological studies also showed that the highest rose petal ointment had more developed hair follicles and denser collagen fibers like the standard group. Overall, it can be concluded that the rose petal methanol extract as an ointment has a very good wound healing effect.