Every culture has gained a global vision at some point in its history. As well as various ways of experiencing reality, it also refers to a distinct set of thoughts, values, and concepts. Negotiating international business is a complex economic activity that involves transnational and cross-cultural interactions. The negotiation of international business transactions is more challenging than the negotiation of commercial transactions in a single cultural milieu because it demands different ways of thinking, feeling, and acting. As a result, in order to develop strong communication skills, we must begin to observe things from views other than our own and gain a basic understanding of the cultures of those other perspectives. Finally, we might be able to spur economic growth. This paper consists of four parts. It firstly expounds the cultural differences existing in the business negotiation of enterprises. On this basis, it compares the cultural differences between different enterprises, shows influence on international business negotiations and puts forward corresponding countermeasures. The research results of this paper can provide a certain theoretical basis for the development of Chinese enterprises in the international economic and trade field, and provide front-line negotiators with corresponding solutions.