Analysis on the Constituents of Branches, Berries, and Leaves of Hippophae rhamnoides L. by UHPLC-ESI-QTOF-MS and Their Anti-Inflammatory Activities

Abstract

Sea buckthorn ( Hippophae rhamnoides L.) is a medicinal plant widely distributed in Asia and Europe, containing plentiful bioactive substances. Our research aimed to promote the comprehensive utilization of the branches, leaves, and berries of sea buckthorn. Qualitative analysis of chemical constituents in branches, leaves, and berries of sea buckthorn was conducted by ultra-high performance liquid chromatography accurate mass quadrupole time-of-flight mass spectrometry with electrospray ionization (UHPLC-ESI-QTOF-MS). As a result, the branch, leaf, and berry samples could be clearly separated in principal component analysis scores plot, and 24 significant markers were found to distinguish these parts by partial least squares regression discrimination analysis in Mass Profiler Professional software. Meanwhile, the compositional similarity of sea buckthorn leaves and branches was higher than that of leaves and berries. In addition, the inhibition of nitric oxide (NO) production of different parts in macrophage RAW 264.7 cells was carried out. At the concentration of 10 μg/mL, sea buckthorn extracts showed good anti-inflammatory activities with NO inhibition values from 73% to 98%.

Sea Buckthorn Leaf Powders: The Impact of Cultivar and Drying Mode on Antioxidant, Phytochemical, and Chromatic Profile of Valuable Resource

“…In vitro and in vivo studies have confirmed the anti-inflammatory, antitumor, hepatoprotective, immunomodulatory, anti-atherogenic, anti-stress, hepatoprotective, radioprotective, tissue repair, antibacterial, antifungal, antiviral, and antioxidant activities, which are determined by the multichemical origin compounds [ 4 , 9 , 10 , 11 , 12 , 13 ]. Fruits and leaves contain rich profiles of carotenoids, tocopherols, amino acids, triterpenic compounds, and phenolic compounds [ 3 , 5 , 7 , 8 , 10 , 14 , 15 , 16 , 17 , 18 , 19 , 20 ]. The phenolic compounds determine the pharmacological effects that are associated with the antioxidant activity [ 10 , 21 , 22 ].…”

Unique bioactive molecule composition of sea buckthorn (Hippophae rhamnoides L.) oils obtained from the peel, pulp, and seeds via physical “solvent‐free” approaches

“…leaf/leaves, stem/branches, of both plant sexes, as well as the berries and their seeds and peel (pulp-flakes) are rich sources of various valuable phytochemicals (Cenkowski et al, 2006;G orna s, Dwiecki, et al, 2016;Gradt et al, 2017;Michel et al, 2012;Yang & Kallio, 2002). It has been reported that the bioactive compounds contained in the sea buckthorn possess many biological activities such as antioxidant and anti-enzymatic (Tkacz et al, 2019), anti-inflammatory (Zheng et al, 2019), antitumoral, and anticancer (Masoodi et al, 2020) properties. It has also been claimed that sea buckthorn berries are a promising source of probiotic bacteria, such as Lactobacillus rhamnosus and Ehstreptococcus thermophilus, of which inhibit the activation of purine that is used as an energy source by SARS-CoV-2.…”

Phytochemistry and pharmacology of sea buckthorn (Elaeagnus rhamnoides; syn. Hippophae rhamnoides): progress from 2010 to 2021

“…Extracts from fruit of different cultivars of SB efficiently inhibited 15-lipoxygenase (Tkacz et al 2019). Similarly, extracts from the fruit, leaves and twigs of SB exerted efficient inhibitory activity against synthesis of NO Á by LPS-induced RAW264.7 mouse macrophages (Zheng et al 2019). In a more detailed study, kaempferol 3 52.3, 18.2, 26.9, 20.6, 25.1, 17.8, 15.0, 23.6, and 12.5 lM, respectively (Yang et al 2013).…”