Analysis on the dynamic changes of the amount of WSSV in Chinese shrimp Fenneropenaeus chinensis during infection

Sun, Yumiao; Li, Fuhua; Xiang, Jianhai

doi:10.1016/j.aquaculture.2012.11.014

Cited by 86 publications

(50 citation statements)

References 40 publications

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“…After intramuscular injection, WSSV infected hemocytes at an earlier time (during the first 14 h) (Tang and Lightner, 2000), while it began propagation in intestine at a later time, 27 h post infection (Sun et al, 2013). The infection order in hemocytes and intestine was related with in vivo transporting of WSSV.…”

Section: Discussionmentioning

confidence: 98%

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One type of VEGFR is involved in WSSV infection to the Pacific whiteleg shrimp Litopenaeus vannamei

Li¹,

Wang²,

Li³

et al. 2015

Developmental & Comparative Immunology

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Section: Discussionmentioning

confidence: 98%

“…WSSV copy number in the pleopods was quantitatively analyzed according to the method described by Sun et al (2013). Briefly, DNA was extracted from pleopods using the Genomic DNA Kit (Tiangen, China) following recommended protocols.…”

Section: Quantitative Real-time Pcr and Data Analysismentioning

confidence: 99%

One type of VEGFR is involved in WSSV infection to the Pacific whiteleg shrimp Litopenaeus vannamei

Li¹,

Wang²,

Li³

et al. 2015

Developmental & Comparative Immunology

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“…Forty-eight shrimps that died during the fi rst 5 days were classifi ed as the susceptible group (sus), while 48 shrimps that were alive on day 19 after WSSV infection were regarded as the resistant group (res). Samples were stored at -20°C for DNA extraction and the virus copy numbers were detected according to the method described by Sun et al (2013).…”

Section: Samples For Association Studies Between Snps and Wssv Suscepmentioning

confidence: 99%

A new ALF from Litopenaeus vannamei and its SNPs related to WSSV resistance

et al. 2014

Chin. J. Ocean. Limnol.

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Anti-lipopolysaccharide factors (ALFs) are basic components of the crustacean immune system that defend against a range of pathogens. The cDNA sequence of a new ALF, designated nLvALF2 , with an open reading frame encoding 132 amino acids was cloned. Its deduced amino acid sequence contained the conserved functional domain of ALFs, the LPS binding domain (LBD). Its genomic sequence consisted of three exons and four introns. nLvALF2 was mainly expressed in the Oka organ and gills of shrimps. The transcriptional level of nLvALF2 increased signifi cantly after white spot syndrome virus (WSSV) infection, suggesting its important roles in protecting shrimps from WSSV. Single nucleotide polymorphisms (SNPs) were found in the genomic sequence of nLvALF2 , of which 38 were analyzed for associations with the susceptibility/resistance of shrimps to WSSV. The loci g.2422 A>G, g.2466 T>C, and g.2529 G>A were signifi cantly associated with the resistance to WSSV ( P <0.05). These SNP loci could be developed as markers for selection of WSSV-resistant varieties of Litopenaeus vannamei .

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“…Even though, immunoassays using these MAbs demonstrated lower sensitivity than that of PCR, the immunoassays especially the immunochromatographic strip test is a useful tool for surveillance for WSSV infection during farming due to its simplicity, lower cost, rapid diagnostic result and can be done at pond site by farmers themselves (Powell et al 2006;Sithigorngul et al 2006Sithigorngul et al , 2011. A low-level mortality was observed (Sun, Li & Xiang 2013) corresponding to the detection limits of dot blotting in this experiment. Real-time PCR was conducted to quantify WSSV infection of P. vannamei in 30 commercial growout ponds in different farms during summer (July to August) and autumn (October) in Korea in 2007.…”

Section: Discussionmentioning

confidence: 70%

Enhancement and confirmation of white spot syndrome virus detection using monoclonal antibody specific to VP26

et al. 2016

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A portion of the VP26 gene (VP26F109) encoding a structural protein of white spot syndrome virus was expressed, purified by SDS-PAGE and used for immunization of Swiss mice for monoclonal antibody (MAb) production. Three groups of MAbs specific to different epitopes on VP26 were selected; these MAbs can be used to detect natural WSSV infection in Penaeus vannamei using dot blotting, Western blotting or immunohistochemistry without cross-reaction with other shrimp tissues or other common shrimp viruses. The detection sensitivity of the MAbs was ranged 7-14 fmole per spot of the rVP26F109 as determined using dot blotting. A combination of three MAbs specific to VP26 with MAbs specific to VP28, VP19 and ICP11 increased the detection sensitivity of WSSV during early infection. Therefore, the MAbs specific to VP26 could be used to confirm and to enhance the detection sensitivity for WSSV infection in shrimp with various types of antibody-based assays.

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Analysis on the dynamic changes of the amount of WSSV in Chinese shrimp Fenneropenaeus chinensis during infection

Cited by 86 publications

References 40 publications

One type of VEGFR is involved in WSSV infection to the Pacific whiteleg shrimp Litopenaeus vannamei

One type of VEGFR is involved in WSSV infection to the Pacific whiteleg shrimp Litopenaeus vannamei

A new ALF from Litopenaeus vannamei and its SNPs related to WSSV resistance

Enhancement and confirmation of white spot syndrome virus detection using monoclonal antibody specific to VP26

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