Analysis on the expression and function of syndecan in the Pacific white shrimp Litopenaeus vannamei

Yang, Hui; Li, Shihao; Li, Fuhua; Wen, Rong; Xiang, Jianhai

doi:10.1016/j.dci.2015.03.013

Cited by 14 publications

(5 citation statements)

References 46 publications

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“…WSSV infects specific types of hemocytes (Wang et al, 2002) and WSSV causes changes in the expression level of immune-related genes (Wang et al, 2019; Xue et al, 2013). In this study, genes such as LOC122260926 syndecan-like , which had an increase in gene expression by virus infection in total hemocytes, had also previously been reported to have similar increases (Sun et al, 2014; Yang et al, 2015). It is also interesting to note that the syndecan is thought to be involved in the entry of WSSV into cells, and that the expression of these receptors has increased due to viral infection.…”

Section: Discussionsupporting

confidence: 69%

scRNA-seq analysis of hemocytes of penaeid shrimp under virus infection

Koiwai

Kondo

Hirono

2023

Preprint

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The classification of cells in non-model organisms has lagged behind that of model organisms using established cluster of differentiation marker sets. To reduce fish diseases, research is needed to better understand immune-related cells, or hemocytes, in non-model organisms like shrimp and other marine invertebrates. In this study, we used Drop-seq to examine how virus infection affected the populations of hemocytes in kuruma shrimp,Penaeus japonicus, which had been artificially infected with a virus. The findings demonstrated that virus infection reduced particular cell populations in circulating hemolymph and inhibited the expression of antimicrobial peptides. We also identified the gene sets that are likely to be responsible for this reduction. Additionally, we identified functionally unknown genes as novel antimicrobial peptides, and we supported this assumption by the fact that these genes were expressed in the population of hemocytes that expressed other antimicrobial peptides. In addition, we aimed to improve the operability of the experiment by conducting Drop-seq with fixed cells as a template and discussed the impact of methanol fixation on Drop-seq data in comparison to previous results obtained without fixation. These results not only deepen our understanding of the immune system of crustaceans but also demonstrate that single-cell analysis can accelerate research on non-model organisms.

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Section: Discussionsupporting

confidence: 69%

scRNA-seq analysis of hemocytes of penaeid shrimp under virus infection

Koiwai

Kondo

Hirono

2023

Preprint

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“…As a species lacks genome information, full-length coding sequence (CDS) analysis is very important for molecular research in L. vannamei 37–39 . To better assign full-length gene, the CDSs of all unigenes were analysed by BLASTx and ESTScan.…”

Section: Resultsmentioning

confidence: 99%

Transcriptome analysis on the exoskeleton formation in early developmetal stages and reconstruction scenario in growth-moulting in Litopenaeus vannamei

Gao

Wei

Yuan

et al. 2017

Sci Rep

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Exoskeleton construction is an important issue in shrimp. To better understand the molecular mechanism of exoskeleton formation, development and reconstruction, the transcriptome of the entire developmental process in Litopenaeus vannamei, including nine early developmental stages and eight adult-moulting stages, was sequenced and analysed using Illumina RNA-seq technology. A total of 117,539 unigenes were obtained, with 41.2% unigenes predicting the full-length coding sequence. Gene Ontology, Clusters of Orthologous Group (COG), the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis and functional annotation of all unigenes gave a better understanding of the exoskeleton developmental process in L. vannamei. As a result, more than six hundred unigenes related to exoskeleton development were identified both in the early developmental stages and adult-moulting. A cascade of sequential expression events of exoskeleton-related genes were summarized, including exoskeleton formation, regulation, synthesis, degradation, mineral absorption/reabsorption, calcification and hardening. This new insight on major transcriptional events provide a deep understanding for exoskeleton formation and reconstruction in L. vannamei. In conclusion, this is the first study that characterized the integrated transcriptomic profiles cover the entire exoskeleton development from zygote to adult-moulting in a crustacean, and these findings will serve as significant references for exoskeleton developmental biology and aquaculture research.

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“…Probably, the PirB vp subunit may recognize the α glycosidic bond between two sugar units, suggesting that the sequence of the Gal(β1-3 or 4)GlcNAc(α 1-2)Man sugar is essential for the PirB vp subunit interaction with the host structures. The existence of heparan sulfate (HS) chains was reported in shrimp [55]. These are sulfated glycosaminoglycans (GAGs) in syndecans involved in several steps of infection caused by the white spot syndrome virus, including initial attachment and entry of viral proteins in shrimp.…”

Section: Discussionmentioning

confidence: 99%

The B Subunit of PirABvp Toxin Secreted from Vibrio parahaemolyticus Causing AHPND Is an Amino Sugar Specific Lectin

et al. 2020

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Vibrio parahaemolyticus (Vp) is the etiological agent of the acute hepatopancreatic necrosis disease (AHPND) in Penaeus vannamei shrimp. Vp possesses a 63–70 kb conjugative plasmid that encodes the binary toxin PirAvp/PirBvp. The 250 kDa PirABvp complex was purified by affinity chromatography with galactose-sepharose 4B and on a stroma from glutaraldehyde-fixed rat erythrocytes column, as a heterotetramer of PirAvp and PirBvp subunits. In addition, recombinant pirB (rPirBvp) and pirA (rPirAvp) were obtained. The homogeneity of the purified protein was determined by SDS-PAGE analysis, and the yield of protein was 488 ng/100 μg of total protein of extracellular products. The PirABvp complex and the rPirBvp showed hemagglutinating activity toward rat erythrocytes. The rPirAvp showed no hemagglutinating capacity toward the animal red cells tested. Among different mono and disaccharides tested, only GalNH2 and GlcNH2 were able to inhibit hemagglutination of the PirABvp complex and the rPirBvp. Glycoproteins showed inhibitory specificity, and fetuin was the glycoprotein that showed the highest inhibition. Other glycoproteins, such as mucin, and glycosaminoglycans, such as heparin, also inhibited the activity. Desialylation of erythrocytes enhanced the hemagglutinating activity. This confirms that Gal or Gal (β1,4) GlcNAc are the main ligands for PirABvp. The agglutinating activity of the PirABvp complex and the rPirBvp is not dependent on cations, because addition of Mg2+ or Ca2+ showed no effect on the protein capacity. Our results strongly suggest that the PirBvp subunit is a lectin, which is part of the PirA/PirBvp complex, and it seems to participate in bacterial pathogenicity.

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Analysis on the expression and function of syndecan in the Pacific white shrimp Litopenaeus vannamei

Cited by 14 publications

References 46 publications

scRNA-seq analysis of hemocytes of penaeid shrimp under virus infection

scRNA-seq analysis of hemocytes of penaeid shrimp under virus infection

Transcriptome analysis on the exoskeleton formation in early developmetal stages and reconstruction scenario in growth-moulting in Litopenaeus vannamei

The B Subunit of PirABvp Toxin Secreted from Vibrio parahaemolyticus Causing AHPND Is an Amino Sugar Specific Lectin

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