Analysis on the promoter region of human decidual prolactin gene in the progesterone-induced decidualization and cAMP-induced decidualization of human endometrial stromal cells

Abstract

These results suggest that cAMP-induced region of the human dPRL promoter resides between -1862 and -1856, -1703 and -1697, -310 and -285, and that the sequences between -1862 and -1856, -1703 and -1697 of the promoter display E + P-induced promoter activity. Furthermore, the current study indicates that E + P or cAMP cooperatively regulate the dPRL gene transcription through some transcriptional factors such as C/EBP, CREB, and other cofactor(s), and that some repressor(s) or corepressor(s) may be involved in the C/EBP-binding site of the human dPRL promoter.

Importance of C/EBPβ Binding and Histone Acetylation Status in the Promoter Regions for Induction of IGFBP-1, PRL, and Mn-SOD by cAMP in Human Endometrial Stromal Cells

“…These findings suggest that C/EBP␤ is a critical transcription factor in human ESCs undergoing decidualization. C/EBP␤ was previously shown to regulate the expression of decidual PRL gene in human ESCs (22,23). The present results, using a ChIP assay and siRNA-mediated C/EBP␤ knockdown, showed in detailed molecular mechanism that induction of PRL gene by cAMP is mediated by the recruitment of C/EBP␤ to the PRL promoter region.…”

“…Therefore, ChIP primers were designed to cover the IRS (IGFBP-1 promoter primer, Ϫ49 to Ϫ212 bp) ( Figure 3A). The PRL promoter region between Ϫ285 and Ϫ310 bp contains the cis-regulatory element for C/EBP␤, which mediates cAMP-induced PRL gene expression (22,23). 278 Tamura et al C/EBP␤ and Histone Modification Endocrinology, January 2014, 155(1):275-286…”

Diverse Roles of Prostaglandins in Blastocyst Implantation

“…In rodents, this process is initiated by the implanting blastocyst [34] while in humans decidualization begins immediately following ovulation, reaches the peak in the mid-luteal phase of the menstrual cycle [49], in response to progesterone, and is independent of the blastocyst signal [50]. Transformation of the stromal fibroblasts into decidual cells can first be seen in the vicinity of the terminal spiral arteries which then spread throughout the endometrial compartment.…”

“…Phosphorylation will also recruit coactivator CREB binding protein (CBP) to the promoter region of the target genes [50], facilitating DNA transcription [66]. A rise in intracellular cAMP will cause Ca 2+ to enter the cell via TRPC1 channel which is essential for the initiation of decidualization [67].…”

“…C/EBP β is also essential for the cAMP signaling [96]. C/EBP β mediates activation of the decidual PRL promoter, resulting in the transcription of dPRL gene [50]. In humans, a functional link between C/EBP β and STAT3 was found to be crucial in the regulation of endometrial stromal cell differentiation [97].…”