Analytical and clinical performance of a fluorescence enzyme immunoassay for progesterone and determination of ovulation day in bitches

Milani, Chiara; Boscato, Elisa L.; Gabai, Gianfranco; Badon, Tamara; Schrank, Magdalena; Sontaş, Hasan; Romagnoli, Stefano; Mollo, Antonio

doi:10.1177/10406387221125466

Cited by 9 publications

(8 citation statements)

References 14 publications

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“…However, in the case of TSH, fT4, and TT3, previous studies have reported the use of these methods in dogs. [42][43][44][45][46][47][48] Additionally, for all of the methods applied, internal validation demonstrated good accuracy (coefficient of variation < 7%). The decision to make the sample population as homogeneous as possible in terms of age, physical activity, and breed certainly lent more robustness to the correlations identified but decreased the possibility of identifying subjects that, because of borderline thyroid function, had cycle phase-related variability that may have affected the proper diagnosis of euthyroidism.…”

Section: Discussionmentioning

confidence: 86%

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Relationship between thyroid function and sex hormones in female German shepherd dogs

Scarpa,

Tagliasacchi,

Iavazzo

et al. 2023

Veterinary Internal Medicne

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BackgroundSeveral variables influence the serum concentration of thyroid hormones in dogs, including breed, age, drugs, and concurrent diseases. However, data regarding the interaction between thyroid function and the estrous stage of female dogs are limited.HypothesisEstrous stage may influence thyroid function in German Shepherd dogs.MethodsLongitudinal, observational, non‐randomized cohort study. The dogs were monitored during the complete estrous cycle, and different stages were determined by vaginal cytology. Two blood samples were collected at the beginning and end of each stage to analyze the following: total thyroxine (TT4), free thyroxine (fT4), total triiodothyronine (TT3), free triiodothyronine (fT3), canine thyrotropin (cTSH), progesterone, 17‐β‐estradiol, triglycerides, and cholesterol concentrations. Hematological and biochemical evaluations were performed at the beginning and end of the study period.AnimalsSeventeen German Shepherds were included, of which 7 were bred during the study period. One dog was excluded for estrus interruption and another for suspected hypothyroidism.ResultsSerum concentrations of T4, fT4, and fT3 were negatively correlated with age. Total thyroxine demonstrated significant changes in serum concentrations between estrous stages, with higher concentrations in estrus and diestrus. Total thyroxine concentrations were positively correlated with progesterone concentrations and negatively correlated with 17‐β‐estradiol concentrations. Free thyroxine did not show significant variations but was positively correlated with progesterone concentrations. Canine TSH concentrations were positively correlated with 17‐β‐estradiol concentrations. No significant differences in thyroid hormones and cTSH concentrations were observed between diestrus during pregnancy and pseudopregnancy.ConclusionsDifferent stages of estrus can influence the measurement of TT4 in female dogs.

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Section: Discussionmentioning

confidence: 86%

“…This situation represents another limitation. However, in the case of TSH, fT4, and TT3, previous studies have reported the use of these methods in dogs 42‐48 . Additionally, for all of the methods applied, internal validation demonstrated good accuracy (coefficient of variation < 7%).…”

Section: Discussionmentioning

confidence: 92%

Relationship between thyroid function and sex hormones in female German shepherd dogs

Scarpa,

Tagliasacchi,

Iavazzo

et al. 2023

Veterinary Internal Medicne

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“…In view of the diverse requirements for precise diagnostics and well-considered decision-making, veterinarians consider commercial immunological analyzers indispensable. Several researchers have reported the accuracy of other commercial point-of-care immunologic analyzers compared with gold-standard methods, such as RIA, LC-MS, and computed tomography (CLIA) [ 5 , 18 – 20 , 30 ]. However, the present study is the first to report the use of the Vcheck ® analyzer, providing novel insights into its accuracy and application.…”

Section: Discussionmentioning

confidence: 99%

Assessment of Vcheck® analyzer for rapid progesterone concentration measurement including recommendations for achieving the optimal breeding time in bitches

Wutthiwitthayaphon,

Suwannachote,

Arayatham

et al. 2024

Vet World

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Background and Aim: Serum progesterone concentration plays critical role in determining the optimal breeding time in bitches and diagnosing reproductive-related issues. This study aimed to conduct a comparative analysis of serum progesterone results obtained from commercial point-of-care immunological analyzers, namely, Vcheck®, with those obtained using chemiluminescent microparticle immunoassay (CMIA). Our overarching goal was to evaluate these analyzers’ accuracy and establish standardized guidelines for optimal breeding timing. Materials and Methods: Ninety-four serum samples from bitches were analyzed using the Vcheck® analyzer and compared with CMIA. Thorough documentation included the mean, standard deviation, 95% confidence interval (CI), and minimum and maximum values of serum progesterone concentrations. Furthermore, Pearson’s correlation coefficient, Lin’s concordance correlation coefficient, and the bias correction factor were meticulously recorded. Results: The mean progesterone concentration measured using the Vcheck® analyzer was significantly lower than that measured using CMIA, with a mean difference of 1.26 ng/mL of serum. The Bias correction factor was 0.935, which was nearly 1.00, indicating that the line of best-fit was on the perfect line of agreement, providing insight into the measurement accuracy. Pearson’s correlation coefficient, a measure of precision, was also close to 1 (0.939), confirming the reliability of the data. Furthermore, Lin’s concordance correlation coefficient was 0.877, indicating a fair overall agreement between the Vcheck® and CMIA methods. These results support the validity of the Vcheck® analyzer’s results. The present study was developed by aligning with established CMIA guidelines and adapting them using the range and 95% CI derived from each set of results, ensuring a standardized and rigorous approach. Conclusion: The Vcheck® analyzer provides a rapid assessment of serum progesterone concentration in bitches, with results comparable to those measured using the CMIA technique. However, when considering the use of the Vcheck® analyzer, it is recommended that the results should be interpreted carefully and the interpretation guidelines should be followed. In conclusion, Vcheck® provides a reliable and convenient method for veterinarian practitioners to measure canine progesterone levels in a clinical/hospital setting. Keywords: chemiluminescent microparticle immunoassay, optimal breeding time, progesterone, Vcheck®.

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“…Singleton pregnancies, fetal heart rate alterations with values lower than 160 bpm ( 23 ), fetuses with US evidence of morphological abnormalities, a stillbirth rate ≥ 30%, the neonatal death of the monitored fetuses or elective C-section, performed before serum progesterone drop (progesterone > 2 ng/ml) were also considered as exclusion criteria. For progesterone assay, the serum samples were analyzed with a fluorescence enzyme immunoassay (FEIA) method using an Automated Immunoassay Analyzer 360 (AIA ® 360, TOSOH Corp., Japan) ( 24 ). Therefore, bitches in which the US monitoring days were based on fetometry were excluded if, after delivery, the US monitoring days counted backward from the parturition day (day 0) were not in the defined time range.…”

Section: Methodsmentioning

confidence: 99%

Ultrasonographic changes in fetal gastrointestinal motility during the last ten days before parturition in dogs

et al. 2022

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Fetal gastrointestinal motility (FGM) was suggested as useful to assess fetal maturity. Our study aimed to quantify FGM in relation to days before parturition (DBP), maternal size, and sex ratio of pups. During the last ten days of pregnancy, 23 clinically healthy pregnant bitches of 16 different breeds ranging in age from 2 to 9 years and body weight from 3.5 to 56.8 kg were monitored twice. The fetal intestine was observed in longitudinal and transversal scan on 3 of the most caudal fetuses in both uterine horns. Gestational age was counted backward from parturition day. The number of fetuses showing FGM was recorded in time in I (−11/−5 DBP) and II (−4/0 DBP). A Mann–Whitney test was performed to analyze variations of FGM% in relation to time and parity. A Kruskal–Wallis test was performed to identify variations of FGM% in relation to maternal size and sex ratio. Statistical significance was set at α = 0.05. A total of 147 FGM observations on 50 ultrasonographic monitoring points were performed. The FGM% was higher during time II compared to time I (median: 33%, range 0–100% vs. 100%, range 33–100%; P < 0.0001). FGM% was higher in small compared to large size bitches (median: 100%, range 67–100% vs. 67%, range 0–100%; P = 0.01). FGM% was not affected by parity and sex ratio. As previously reported, a significant increase in FGM% was observed in the last five DBP. FGM observation may be influenced by the maternal size, with easier evaluation in small size bitches, as well as ultrasound equipment and positioning.

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Analytical and clinical performance of a fluorescence enzyme immunoassay for progesterone and determination of ovulation day in bitches

Cited by 9 publications

References 14 publications

Relationship between thyroid function and sex hormones in female German shepherd dogs

Relationship between thyroid function and sex hormones in female German shepherd dogs

Assessment of Vcheck® analyzer for rapid progesterone concentration measurement including recommendations for achieving the optimal breeding time in bitches

Ultrasonographic changes in fetal gastrointestinal motility during the last ten days before parturition in dogs

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