Analytical and clinical performance of progesterone receptor antibodies in breast cancer

Calhoun, Benjamin C.; Mosteller, Brian; Warren, Daniel; Smith, Margie H; Rowe, J. Jordi; Lanigan, Christopher; Mrazeck, Karen; Walker, Evan J.; Newell, Amy Hanlon; Jones, R.S.

doi:10.1016/j.anndiagpath.2018.02.007

Cited by 8 publications

(5 citation statements)

References 49 publications

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“…Standard IHC assay of the ER discordant cases with the SP1 clone confirmed one case to be positive as assessed by MTP, while the other one remained negative (data not shown). In previous studies, the anti-human PR rabbit monoclonal 1E2 antibody showed higher analytical affinity for PR with respect to the 636 clone [13], [14]. Standard staining of PR discordant cases with the 1E2 clone confirmed two of the three cases to be positive as assessed by microfluidic assay, while one remained negative as by standard staining (data not shown).…”

Section: Discussionmentioning

confidence: 57%

Microfluidic-based immunohistochemistry for breast cancer diagnosis: a comparative clinical study

et al. 2019

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Breast cancer is a highly heterogeneous disease. The efficacy of tailored therapeutic strategies relies on the precise detection of diagnostic biomarkers by immunohistochemistry (IHC). Therefore, considering the increasing incidence of breast cancer cases, a concomitantly time-efficient and accurate diagnosis is clinically highly relevant. Microfluidics is a promising innovative technology in the field of tissue diagnostic, enabling for rapid, reliable and automated immunostaining. We previously reported the microfluidic-based HER2 (human epidermal growth factor receptor 2) detection in breast carcinomas to greatly correlate with the HER2 gene amplification level. Here, we aimed to develop a panel of microfluidic-based IHC protocols for prognostic and therapeutic markers routinely assessed for breast cancer diagnosis, namely HER2, estrogen/progesterone receptor (ER/PR) and Ki67 proliferation factor. The microfluidic IHC protocol for each marker was optimized to reach high staining quality comparable to the standard procedure, while concomitantly shortening the staining time to 16 minutes-excluding deparaffinization and antigen retrieval step-with a turnaround time reduction up to 7 folds. Comparison of the diagnostic score on 50 formaldehyde-fixed paraffin-embedded breast tumor resections by microfluidic versus standard staining showed high concordance (overall agreement: HER2 94%, ER 95.6%, PR 93.6%, Ki67 93.7%) and strong correlation (ρ coefficient: ER 0.89, PR 0.88, ki67 0.87; p<0.0001) for all the analyzed markers. Importantly, HER2 genetic reflex test for all discordant cases confirmed the scores obtained by the microfluidic technique. Overall, the microfluidic-based IHC represents a clinically validated equivalent approach to the standard chromogenic staining for rapid, accurate and automated breast cancer diagnosis.

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Section: Discussionmentioning

confidence: 57%

Microfluidic-based immunohistochemistry for breast cancer diagnosis: a comparative clinical study

et al. 2019

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“…Immunohistochemistry was performed using a VENTANA automated slide stainer (Roche Tissue Diagnostic, Almere, The Netherlands) according to the protocol from the manufacturer. All antibodies (Roche Tissue Diagnostic) 29 were used for clinical diagnosis and listed in Table 2 . For scoring purposes, all slides were subsequently scanned by using 3DHistech PANNORAMIC 250 Flash III DX (3DHistech, Budapest, Hungary) with 40× optical magnification at the Light and Electron Microscopy Facility of the LUMC.…”

Section: Methodsmentioning

confidence: 99%

Differential Expression of Sex-Steroid Receptors in the Choroid Aligns With Central Serous Chorioretinopathy Sex Prevalence Across Different Ages

Galuh,

Meijer,

Brinks

et al. 2024

Invest. Ophthalmol. Vis. Sci.

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“…SPR epitope binding analysis was done as previously described [ 25 , 26 ]. Briefly, SPR kinetic analyses of peptide binding of both clones was performed on a BIAcore T200 using Series S CM5 sensor chips.…”

Section: Methodsmentioning

confidence: 99%

High-Resolution Epitope Mapping and Affinity Binding Analysis Comparing a New Anti-Human LAG3 Rabbit Antibody Clone to the Commonly Used Mouse 17B4 Clone

et al. 2022

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Lymphocyte activation gene 3 (LAG3) is a T cell inhibitory receptor that promotes tumor cell immune escape and is a potential target for cancer diagnostic and immunotherapeutic applications. We used automated capillary electrophoresis (ACE), surface plasmon resonance (SPR), and immunohistochemistry (IHC) to compare the binding characteristics of a new anti-LAG3 rabbit antibody clone, SP464, with the thirty-year old and extensively used anti-LAG3 mouse 17B4 clone. The rabbit SP464 clone exhibited between 20× to 30× greater binding to LAG3 than did the mouse 17B4 clone. Using these tools, we precisely mapped the relative locations of the epitopes of these two antibodies. The SP464 and 17B4 minimal epitopes were localized to separate, but overlapping, sub-fragments within the amino-terminal fifteen acids of the original thirty-mer peptide immunogen used to generate both antibodies. Application of this approach for quantifying the effects of alanine substitutions along the minimal SP464 epitope identified two amino acids essential for binding and four amino acids that likely contribute towards binding. Together, ACE, SPR, and IHC constitute a powerful orthologous approach for comparing antibody-binding characteristics and for fine mapping of linear epitopes within short immunogens. Our results indicate that the rabbit clone SP464 may be useful for assessing LAG3 expression.

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Analytical and clinical performance of progesterone receptor antibodies in breast cancer

Cited by 8 publications

References 49 publications

Microfluidic-based immunohistochemistry for breast cancer diagnosis: a comparative clinical study

Microfluidic-based immunohistochemistry for breast cancer diagnosis: a comparative clinical study

Differential Expression of Sex-Steroid Receptors in the Choroid Aligns With Central Serous Chorioretinopathy Sex Prevalence Across Different Ages

High-Resolution Epitope Mapping and Affinity Binding Analysis Comparing a New Anti-Human LAG3 Rabbit Antibody Clone to the Commonly Used Mouse 17B4 Clone

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