Analytical and Preparative Polyacrylamide Gel Electrophoresis

Chrambach, Andreas; Rodbard, David; Jovin, Thomas M.; Svendsen, P. Just

doi:10.1007/978-1-4684-9984-1_2

Cited by 79 publications

(22 citation statements)

References 77 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Immunoblotting procedures were as detailed in [12], using the three antisera (at dilutions of 1:500-1:2500) and goat anti-rabbit lgG-peroxidase conjugate diluted 1:2500. The blots were exposed to 0.01070 hydrogen peroxide, 0.6 mg/ml 4-chloro-l-naphthol in Tris-buffered saline, Nondenaturing gel electrophoresis was performed according to [13], using 507o polyacrylamide as the running gel. The proteins were prepared in sample buffer (5007o sucrose solution, 0.1% bromophenol blue), loaded onto the gel, and electrophoresed at constant power [13].…”

Section: Methodsmentioning

confidence: 99%

Identification of two distinct protein carboxyl methyltransferases in eucaryotic cells

et al. 1988

View full text Add to dashboard Cite

Two distinct protein carboxyl methyltransferases (PCM) were identified in the electric organ of Torpedo ocellata. They were separated from each other in the active form by means of nondenaturing gel electrophoresis and by p-(chloromercufi)benzoate-agarose chromatography, and were individually identified by specific polyclonal antibodies. The existence of at least two distinct PCMs in eucaryotic cells raises the possibility that these enzymes are involved in distinct transmethylation reactions.Protein carboxyl methyltransferase; Protein methylation

show abstract

Section: Methodsmentioning

confidence: 99%

Identification of two distinct protein carboxyl methyltransferases in eucaryotic cells

et al. 1988

View full text Add to dashboard Cite

show abstract

“…Procedures of polymerization and of electrophoresis were those previously described [26,27]. Polyacrylamide gel electrophoresis was carried out in multiphasic zone electrophoresis buffer systems B [27], 2274.3(X2).…”

Section: Poiyacrylamide Gel Electrophoresismentioning

confidence: 99%

“…Polyacrylamide gel electrophoresis was carried out in multiphasic zone electrophoresis buffer systems B [27], 2274.3(X2). 0 and 1954.2.0 [26,29,31] at 0 "C. A jacketed tube apparatus made of pyrex [26] and one made of Plexiglas (Hoefer Scientific, San Francisco, Calif.) were used for analytical polyacrylamide gel electrophoresis.…”

Section: Poiyacrylamide Gel Electrophoresismentioning

confidence: 99%

“…Data analysis was carried out using programs of D. Rodbard (the 'polyacrylamide gel electrophoresispack') as described [26,27,35]. Specifically, computed were the regression line of log RF versus % total gel concentration (Ferguson plot), its confidence envelopes, its slope (retardation coefficient, K R , a measure of molecular size), its y-intercept (Yo, a measure of molecular net charge), the joint 95% confidence envelopes of KR and YO [27,35].…”

Section: Poiyacrylamide Gel Electrophoresismentioning

confidence: 99%

“…Focusing times of 4, 6, 17 and 21 h were used. The apparatus was that previously described [26,39]. Staining was carried out by the procedure of Vesterberg [40] or that of Reisner [41].…”

Section: Isoelectric Focusing On Polyacrylamide Gelmentioning

confidence: 99%

See 2 more Smart Citations

Calf‐Ovary Protein Kinases Dependent on Adenosine 3′: 5′‐Monophosphate

Salokangas

Talmadge

Bechtel

et al. 1977

European Journal of Biochemistry

View full text Add to dashboard Cite

High resolving power and quantitative application of polyacrylamide‐gel electrophoresis at various pore sizes and electrofocusing provide resolution of a calf‐ovarian protein‐kinase system at an increased level of magnification, as well as optimal preparative routes. Three protein kinases dependent on adenosine 3′:5′‐monophosphate are distinguished by polyacrylamide gel electrophoresis in calf ovarian cytosol. These enzymes which are observed in the pH range 7.5–10.2, appear to be aggregates of a common subunit or monomer. The three kinases are, by the criteria of polyacrylamide gel electrophoresis, distinct from three adenosine‐3′:5′‐monophosphate‐binding proteins found in the calf ovarian system. Analysis by electrofocusing on polyacrylamide gel shows that conventionally purified preparations of the major kinase of cytosol contain an overwhelming majority of contaminant proteins.

show abstract

Major plasma proteins of larvae of the southwestern corn borer, Diatraea grandiosella

Lenz

Venkatesh

Chippendale

1987

Arch Insect Biochem Physiol

View full text Add to dashboard Cite

The major protein fraction of the larval hemolymph of the southwestern corn borer, Diatraea grandiosella, was shown to be composed of approximately six native proteins, and accounted for up to 55% of the plasma proteins. The apparent molecular weights of these proteins ranged from 350,000 to 500,000, as determined by nondenaturing electrophoresis at a neutral pH. Lower apparent molecular weights were obtained when the major protein fraction was subjected to electrophoresis at a high pH under nondenaturing conditions, indicating that these proteins dissociated under alkaline conditions. The isoelectric points of the major hemolymph proteins fell between 5.6 and 5.9. Denaturing electrophoresis and two-dimensional electrophoresis showed that the native proteins were composed of subunits having apparent molecular weights of 78,000, 84,000, and 87,000. These subunits were the major labeled polypeptides found in the hemolymph of feeding last instar larvae 24 h after they had been injected with [3H]leucine.They were also the major polypeptides synthesized when the fat body of last instar prediapausing larvae was incubated in vitro for 4 h in Grace's medium containing [3H]leucine. lmmunoelectrophoresis confirmed that the major hemolymph proteins were present in the larval fat body. In addition, these proteins were the major proteins present in the hemolymph of diapausing larvae.

show abstract

Analytical and Preparative Polyacrylamide Gel Electrophoresis

Cited by 79 publications

References 77 publications

Identification of two distinct protein carboxyl methyltransferases in eucaryotic cells

Identification of two distinct protein carboxyl methyltransferases in eucaryotic cells

Calf‐Ovary Protein Kinases Dependent on Adenosine 3′: 5′‐Monophosphate

Major plasma proteins of larvae of the southwestern corn borer, Diatraea grandiosella

Contact Info

Product

Resources

About