Analytical Applications of UV-VIS Spectroscopy

Perkampus, H.‐H.

doi:10.1007/978-3-642-77477-5_4

Cited by 41 publications

(46 citation statements)

References 99 publications

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“…The polychromator is an enhanced monochromator, where the wavelength scanning is accomplished by electronic scanning of the multichannel detector. This multichannel dispositive can detect as many wavelengths simultaneously as their number of individual diodes or pixels [ 52 , 53 ].…”

Section: Uv-vis Absorption Spectroscopy and Turbidity Are Initial mentioning

confidence: 99%

Evaluation of Peptide/Protein Self-Assembly and Aggregation by Spectroscopic Methods

2020

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The self-assembly of proteins is an essential process for a variety of cellular functions including cell respiration, mobility and division. On the other hand, protein or peptide misfolding and aggregation is related to the development of Parkinson’s disease and Alzheimer’s disease, among other aggregopathies. As a consequence, significant research efforts are directed towards the understanding of this process. In this review, we are focused on the use of UV-Visible Absorption Spectroscopy, Fluorescence Spectroscopy and Circular Dichroism to evaluate the self-organization of proteins and peptides in solution. These spectroscopic techniques are commonly available in most chemistry and biochemistry research laboratories, and together they are a powerful approach for initial as well as routine evaluation of protein and peptide self-assembly and aggregation under different environmental stimulus. Furthermore, these spectroscopic techniques are even suitable for studying complex systems like those in the food industry or pharmaceutical formulations, providing an overall idea of the folding, self-assembly, and aggregation processes, which is challenging to obtain with high-resolution methods. Here, we compiled and discussed selected examples, together with our results and those that helped us better to understand the process of protein and peptide aggregation. We put particular emphasis on the basic description of the methods as well as on the experimental considerations needed to obtain meaningful information, to help those who are just getting into this exciting area of research. Moreover, this review is particularly useful to those out of the field who would like to improve reproducibility in their cellular and biomedical experiments, especially while working with peptide and protein systems as an external stimulus. Our final aim is to show the power of these low-resolution techniques to improve our understanding of the self-assembly of peptides and proteins and translate this fundamental knowledge in biomedical research or food applications.

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Section: Uv-vis Absorption Spectroscopy and Turbidity Are Initial mentioning

confidence: 99%

Evaluation of Peptide/Protein Self-Assembly and Aggregation by Spectroscopic Methods

2020

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“…monomer concentration c m , function of the c m logarithm. The degree of polymerization was computed as recommended in literature [5][6][7]. Two polymerization curves are shown, out of which one is drawn for a sample set measured immediately after preparation; the degree of polymerization is 1.018 in this case.…”

Section: Resultsmentioning

confidence: 99%

Time Stability Studies of Quinazoline Derivative Designed to Fight Drug Resistance Acquired by Bacteria

Militaru¹,

Smarandache²,

Mahamoud³

et al. 2011

LDDD

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The 3-[2-(dimethylamino)ethyl]-6-nitroquinazolin-4(3H)-one (BG1188) may modify the antibiotic susceptibility of human Gram-negative bacteria which present multidrug resistant phenotype. This application requires to know the time stability of BG1188 solutions. This paper reports for the first time about the time stability of the BG1188 solutions measured by optical absorption; the solvents were ultrapure de-ionized water and dimethyl sulfoxide (DMSO). In ultrapure water, BG1188 concentration varied between 2x10 -3 M (stock solution) and 10 -6 M, at pH = 6.7. In DMSO, the concentration was 10 -3 M (recommended by higher activity on bacteria). Solutions in ultrapure water were kept in dark at 4°C and measured at room temperature (22°C). The ultraviolet-visible (UV-Vis) absorption spectra have shown that only monomer forms were present in solutions. One of the obtained results is that the time evolution of the absorption spectra at 10 -3 M in ultrapure water shows a reproducibility within the measuring errors (±1.045%) for time intervals up to 1032 hours (more than 40 days) after preparation, showing a high stability of BG1188 solutions; generally, samples are stable within the experimental errors at concentrations higher than 10 -5 M, but the stability time interval may be variable: from 119 days at 10 -4 M to 34 days at 10 -5 M.As for solutions in the DMSO, the absorption spectra show that the samples exhibit modifications immediately after preparation, in terms of peak intensities and shapes, so that the BG1188 solutions in DMSO may be considered as unstable.

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“…For instance, Otake et al investigated the activity of a vanadium oxide catalyst supported on metal-organic framework (MOF) for selective alcohol oxidation and used both Raman and IR to characterize the synthesized catalysts, which exhibited the existence of different V-containing bonds before and after reaction and helped reveal that the dehydrated form of V2 species on MOF are actually the active sites [75]. While the interaction of molecules with infrared light causes vibrational transitions (as in the case of IR), the UV (200-400 nm) and visible light (400-700 nm) with shorter wavelength but higher energy radiation would cause electronic transitions to molecules, which means that the molecules could adsorb certain wavelength of light that matches the energy difference between the possible excited state and ground state of molecules [76]. By measuring the intensity of light before and after the light passes through the sample, UV-vis spectrometer can be used to determine qualitatively and quantitatively the concentration of ions or organic compounds based on the Beer-Lambert Law [77].…”

Section: Experimental Approach For Studying Active Sitesmentioning

confidence: 99%

Active Sites in Heterogeneous Catalytic Reaction on Metal and Metal Oxide: Theory and Practice

et al. 2018

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Active sites play an essential role in heterogeneous catalysis and largely determine the reaction properties. Yet identification and study of the active sites remain challenging owing to their dynamic behaviors during catalysis process and issues with current characterization techniques. This article provides a short review of research progresses in active sites of metal and metal oxide catalysts, which covers the past achievements, current research status, and perspectives in this research field. In particular, the concepts and theories of active sites are introduced. Major experimental and computational approaches that are used in active site study are summarized, with their applications and limitations being discussed. An outlook of future research direction in both experimental and computational catalysis research is provided.

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Analytical Applications of UV-VIS Spectroscopy

Cited by 41 publications

References 99 publications

Evaluation of Peptide/Protein Self-Assembly and Aggregation by Spectroscopic Methods

Evaluation of Peptide/Protein Self-Assembly and Aggregation by Spectroscopic Methods

Time Stability Studies of Quinazoline Derivative Designed to Fight Drug Resistance Acquired by Bacteria

Active Sites in Heterogeneous Catalytic Reaction on Metal and Metal Oxide: Theory and Practice

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