Analytical approaches to determination of carnitine in biological materials, foods and dietary supplements

Dąbrowska, Monika; Starek, Małgorzata

doi:10.1016/j.foodchem.2013.06.137

Cited by 45 publications

(30 citation statements)

References 63 publications

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“…Chirality becomes increasingly important particularly in the pharmaceutical field, because in most cases only one of the enantiomers possesses the desired pharmacological effects while the other one may be toxic, less active, and/or possesses undesirable side effects, or even act as an antagonist . Therefore, the development of an enantioseparation method is of great importance not only to avoid unwanted pharmaceutical and/or toxicological side effects but also to guarantee its therapeutic efficacy and safety .…”

Section: Introductionmentioning

confidence: 99%

Chiral separation of 12 pairs of enantiomers by capillary electrophoresis using heptakis-(2,3-diacetyl-6-sulfato)-β-cyclodextrin as the chiral selector and the elucidation of the chiral recognition mechanism by computational methods

et al. 2017

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Chiral separation of 12 pairs of basic analyte enantiomers including oxybutynin, bambuterol, tradinterol, clenbuterol, clorprenaline, terbutaline, tulobuterol, citalopram, phencynonate, fexofenadine, salbutamol, and penehyclidine was conducted by capillary electrophoresis using a single-isomer anionic β-cyclodextrin derivative, heptakis-(2,3-diacetyl-6-sulfato)-β-cyclodextrin as the chiral selector. Parameters influencing separation were studied, including background electrolyte pH, heptakis-(2,3-diacetyl-6-sulfato)-β-cyclodextrin concentration, buffer concentration, and separation voltage. A background electrolyte consisting of 50 mM Tris-H PO and 6 mM heptakis-(2,3-diacetyl-6-sulfato)-β-cyclodextrin at pH 2.5 was found to be highly efficient for the separation of most enantiomers, with other conditions of normal polarity mode at 10 kV, detection wavelength of 210 nm using hydrodynamic injection for 3 s. Under the optimal conditions, baseline resolution (>1.50) for 11 pairs of enantiomers and somewhat lower resolution for penehyclidine enantiomers (1.17) were generated. Moreover, the possible mechanism of separation of clenbuterol, oxybutynin, salbutamol, and penehyclidine was investigated using a computational modeling method.

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Section: Introductionmentioning

confidence: 99%

Chiral separation of 12 pairs of enantiomers by capillary electrophoresis using heptakis-(2,3-diacetyl-6-sulfato)-β-cyclodextrin as the chiral selector and the elucidation of the chiral recognition mechanism by computational methods

et al. 2017

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“…[1][2][3] High performance liquid chromatography (HPLC) is a well-known and widely used analytical technique that is prevalent throughout the pharmaceutical field as a research tool for enantioseparation. [1][2][3] High performance liquid chromatography (HPLC) is a well-known and widely used analytical technique that is prevalent throughout the pharmaceutical field as a research tool for enantioseparation.…”

Section: Introductionmentioning

confidence: 99%

Enantioseparation of nine indanone and tetralone derivatives by HPLC using carboxymethyl‐β‐cyclodextrin as the mobile phase additive

Guo

Sun

et al. 2016

Chirality

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High-performance liquid chromatography (HPLC) is a powerful method in the area of chiral separation. In this study, a method of HPLC using carboxymethyl-β-cyclodextrin (CM-β-CD) as chiral selector was developed for enantioseparation of nine indanone and tetralone derivatives. The separation was performed on a conventional C column. The optimal mobile phase was a mixture of methanol and 0.05 mol/L phosphate buffer at pH 1.8 (55:45, v/v) containing 22.9 mmol/L CM-β-CD. Under such conditions, the resolutions of all analytes were over 1.8 except for Compound F. The results of the study indicate the presence of a complex with 1:1 stoichiometry of the inclusion complex. In addition, it can be inferred from thermodynamic analysis that the behavior of formation of the inclusion complex and enantioseparation occurred simultaneously, while they were driven by different forces. The effect of analyte structure is also discussed.

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“…LCarnitine ((-)-b-hydroxy-c-(trimethylammonio)butyrate), the biologically active carnitine stereoisomer, plays a vital role in the metabolism of fatty acids, as acyltransferase cofactor, and also in energy production processes of the human metabolism [1,2]. Other functions of Lcarnitine include modulating the mitochondrial acylCoA/CoA [3] ratio, the storage of energy in the form of acetylcarnitine [4], and the modulation of the toxic effects of poorly metabolized acyl groups, which are excreted as carnitine esters [5].…”

Section: Introductionmentioning

confidence: 99%

“…The most common analytical methods reported in the literature for the determination of L-carnitine are chromatographic methods [1,[13][14][15] in which L-carnitine and its esters require prior derivatization followed by UV [14] or fluorescent detection [15]. This last technique has also been used together with capillary electrophoresis [16] and flow injection analysis [17] with slightly lower detection limits.…”

Section: Introductionmentioning

confidence: 99%

“…This last technique has also been used together with capillary electrophoresis [16] and flow injection analysis [17] with slightly lower detection limits. A recent review surveys the progress made in carnitine determination in biological materials, foods, and dietary supplements [1]. However, to the best of our knowledge, only one paper has described the use of potentiometric selective electrodes, basing on macrocyclic antibiotics, for detecting L-carnitine [18].…”

Section: Introductionmentioning

confidence: 99%

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Ion-selective electrodes for the determination of l-carnitine. Application in dissolution testing of a dietary supplement

et al. 2014

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L-Carnitine selective electrodes were developed using polymeric membranes containing a lipophilic cation exchanger (potassium tetrakis[3,5-bis(trifluoromethyl) phenyl]borate) and plasticized with three different plasticizers: bis(2-ethylhexyl) sebacate, tricresyl phosphate, and 2-nitrophenyl octyl ether. The response of the electrodes was evaluated in terms of calibration parameters (slope, limit of detection, and linear range), reproducibility, selectivity profile, and pH influence. The electrodes were applied to the dissolution testing of a dietary supplement containing L-carnitine in water and in HCl (pH = 3) by direct potentiometric monitoring.

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Analytical approaches to determination of carnitine in biological materials, foods and dietary supplements

Cited by 45 publications

References 63 publications

Chiral separation of 12 pairs of enantiomers by capillary electrophoresis using heptakis-(2,3-diacetyl-6-sulfato)-β-cyclodextrin as the chiral selector and the elucidation of the chiral recognition mechanism by computational methods

Chiral separation of 12 pairs of enantiomers by capillary electrophoresis using heptakis-(2,3-diacetyl-6-sulfato)-β-cyclodextrin as the chiral selector and the elucidation of the chiral recognition mechanism by computational methods

Enantioseparation of nine indanone and tetralone derivatives by HPLC using carboxymethyl‐β‐cyclodextrin as the mobile phase additive

Ion-selective electrodes for the determination of l-carnitine. Application in dissolution testing of a dietary supplement

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