Analytical Biosensing of Hydrogen Peroxide on Brilliant Cresyl Blue/Multiwalled Carbon Nanotubes Modified Glassy Carbon Electrode

Abstract

A cationic quinine-imide dye brilliant cresyl blue (BCB) and horseradish peroxidase (HRP) were co-immobilized within ormosil on multiwalled carbon nanotubes modified glassy carbon electrode for the fabrication of highly sensitive and selective hydrogen peroxide biosensor. The presence of epoxy group in ormosil as organic moiety improves the mechanical strength and transparency of the film and amino group provides biocompatible microenvironment for the immobilization of enzyme. The presence of MWCNTs improved the conductivity of the nanocomposite film. The surface characterization of MWCNT modified ormosil nanocomposite film was performed with scanning electron microscopy (SEM) and atomic force microscopy (AFM). Cyclic voltammetry and amperometry measurements were used to study and optimize the performance of the resulting peroxide biosensor. The apparent Michaelis -Menten constant was determined to be 1.5 mM. The proposed H 2 O 2 biosensor exhibited wide linear range from 3 Â 10 À7 to 1 Â 10 À4 M, and low detection limit 1 Â 10 À7 M (S/N ¼ 3) with fast response time < 5 s. The probable interferences in bio-matrix were selected to test the selectivity and no significant response was observed in the biosensor. This biosensor possessed good analytical performance and long term storage stability.

A hydrogen peroxide sensor based on the nanocomposites of poly(brilliant cresyl blue) and single walled-carbon nanotubes

“…Brilliant cresyl blue (BCB), one of the phenoxazine dyes, has been reported on a biosensor consisting of horseradish peroxidase/brilliant cresyl blue/multiwalled carbon nanotubes for the amperometric determination of H 2 O 2 . 27,28 Polymerization of brilliant cresyl blue has been shown to be an alternative method in the configuration of sensors to overcome the leaching out of the mediator from the electrode surface. 29 Poly(brilliant cresyl blue) (PBCB) functionalized multiwalled carbon nanotubes have been reported to improve the sensitivity in determining epinephrine.…”

A nanoscaled Au–horseradish peroxidase composite fabricated by an interface reaction and its characterization, immobilization and biosensing

“…The apparent Michaelis-Menten constant (K app M ), which indicates the enzyme-substrate kinetics, can be obtained from the Lineweaver-Burk equation. 42 It was found to be 0.14 mM, which was smaller than 1.5 mM for the H 2 O 2 biosensor fabricated by immobilizing HRP on the multiwalled carbon nanotube modied glassy carbon electrode (GCE) 43 and 1.55 mM for the H 2 O 2 biosensor fabricated by immobilizing HRP on nano-Au with the choline covalently modied GCE. 44 It indicated that the prepared Au-HRP modied electrode exhibited a higher affinity for H 2 O 2 .…”

Hydrogen Peroxide Biosensors Based on Horseradish Peroxidase and Hemoglobin

“…It is still better compared with the Layer-by-layer (LBL) assembly of the nanocomposite of Methylene Blue-Multiwalled Carbon Nanotubes (MB-MWNTs) and HRP [43]. However, a higher sensitive H 2 O 2 biosensor can be constructed by using the nanocomposite of brilliant cresyl blue-MWCNTs [44], nile blue-MWCNTs [45] and toluidine blue-MWCNTs [46], methylene blue-MWCNTs [47]. Moreover, a very high sensitive H 2 O 2 biosensor can be constructed by wrapping the nanocomposite of MB-Sodium Dodecylsulfate (SDS) with the MWCNTs [48].…”