Analytical Considerations for Microdialysis Sampling

Nandi, Pradyot; Kuhnline, Courtney D.; Lunte, Susan M.

doi:10.1002/9781118011294.ch3

Cited by 3 publications

(2 citation statements)

References 218 publications

(227 reference statements)

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“…Since it is generally assumed that the mass transfer resistance is primarily resulting from the resistance implied by the membrane , simplification leads to:

R_{m} > > R_{e, p}

R = R_{m} = 21.6 \min / μ normalL

…”

Section: Resultsmentioning

confidence: 99%

Development of a highly sensitive gas chromatography–mass spectrometry method preceded by solid‐phase microextraction for the analysis of propofol in low‐volume cerebral microdialysate samples

Guntner

Stöcklegger

Kneidinger

et al. 2019

J of Separation Science

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To date, the commonly used intravenous anesthetic propofol has been widely studied, and fundamental pharmacodynamic and pharmacokinetic characteristics of the drug are known. However, propofol has not yet been quantified in vivo in the target organ, the human brain. Here, cerebral microdialysis offers the unique opportunity to sample propofol in the living human organism. Therefore, a highly sensitive analytical method for propofol quantitation in small sample volumes of 30 μL, based on direct immersion solid‐phase microextraction was developed. Preconcentration was followed by gas chromatographic separation and mass spectrometric detection of the compound. This optimized method provided a linear range between the lower limit of detection (50 ng/L) and 200 μg/L. Matrix‐matched calibration was used to compensate recovery issues. A precision of 2.7% relative standard deviation between five consecutive measurements and an interday precision of 6.4% relative standard deviation could be achieved. Furthermore, the permeability of propofol through a cerebral microdialysate system was tested. In summary, the developed method to analyze cerebral microdialysate samples, allows the in vivo quantitation of propofol in the living human brain. Additionally the calculation of extracellular fluid levels is enabled since the recovery of the cerebral microdialysis regarding propofol was determined.

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“…Since it is generally assumed that the mass transfer resistance is primarily resulting from the resistance implied by the membrane , simplification leads to:

R_{m} > > R_{e, p}

R = R_{m} = 21.6 \min / μ normalL

…”

Section: Resultsmentioning

confidence: 99%

Development of a highly sensitive gas chromatography–mass spectrometry method preceded by solid‐phase microextraction for the analysis of propofol in low‐volume cerebral microdialysate samples

Guntner

Stöcklegger

Kneidinger

et al. 2019

J of Separation Science

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“…There are four main methods for microdialysis probe calibration: 1) determining recovery in vitro and assuming that it is the same in vivo , 2) delivering an internal standard in the perfusate and accounting for its loss [55-57], 3) using the no-net-flux and dynamic no-net-flux methods [58-61], and 4) employing ultra-low flow rates (~0.1 μL/min) [54] or the MetaQuant probe [49,62] and assuming 100% recovery. For addition information on these methods, the reader is directed to excellent reviews on the topic [52,63]. …”

Section: Microdialysis Samplingmentioning

confidence: 99%

A review of microdialysis coupled to microchip electrophoresis for monitoring biological events

Saylor

Lunte

2015

Journal of Chromatography A

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Microdialysis is a powerful sampling technique that enables monitoring of dynamic processes in vitro and in vivo. The combination of microdialysis with chromatographic or electrophoretic methods yields along with selective detection methods yields a “separation-based sensor” capable of monitoring multiple analytes in near real time. Analysis of microdialysis samples requires techniques that are fast (<1 min), have low volume requirements (nL–pL), and, ideally, can be employed on-line. Microchip electrophoresis fulfills these requirements and also permits the possibility of integrating sample preparation and manipulation with detection strategies directly on-chip. Microdialysis coupled to microchip electrophoresis has been employed for monitoring biological events in vivo and in vitro. This review discusses technical considerations for coupling microdialysis sampling and microchip electrophoresis, including various interface designs, and current applications in the field.

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Microdialysis Sampling Coupled to Microchip Electrophoresis with Integrated Amperometric Detection on an All‐Glass Substrate

2013

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The development of an all-glass separation-based sensor using microdialysis coupled to microchip electrophoresis with amperometric detection is described. The system includes a flow-gated interface to inject discrete sample plugs from the microdialysis perfusate into the microchip electrophoresis system. Electrochemical detection was accomplished with a platinum electrode in an in-channel configuration using a wireless electrically isolated potentiostat. To facilitate bonding around the in-channel electrode, a fabrication process was employed that produced a working and a reference electrode flush with the glass surface. Both normal and reversed polarity separations were performed with this sensor. The system was evaluated in vitro for the continuous monitoring of the production of hydrogen peroxide from the reaction of glucose oxidase with glucose. Microdialysis experiments were performed using a BASi loop probe with an overall lag time of approximately five minutes and a rise time of less than 60 seconds.

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Analytical Considerations for Microdialysis Sampling

Cited by 3 publications

References 218 publications

Development of a highly sensitive gas chromatography–mass spectrometry method preceded by solid‐phase microextraction for the analysis of propofol in low‐volume cerebral microdialysate samples

Development of a highly sensitive gas chromatography–mass spectrometry method preceded by solid‐phase microextraction for the analysis of propofol in low‐volume cerebral microdialysate samples

A review of microdialysis coupled to microchip electrophoresis for monitoring biological events

Microdialysis Sampling Coupled to Microchip Electrophoresis with Integrated Amperometric Detection on an All‐Glass Substrate

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