Analytical implications of different methods for preparing plant cell wall material

Fangel, Jonatan U.; Jones, Catherine Y.; Ulvskov, Peter; Harholt, Jesper; Willats, William G. T.

doi:10.1016/j.carbpol.2021.117866

Cited by 14 publications

(16 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Earlier studies have used composite monosaccharide analyses of the inner leaf mesophyll as a proxy for the more complex polysaccharides, but this approach does not adequately represent the tertiary structure of the functional compounds (Grace, Dzajic, et al, 2013). A newer approach to target tertiary structures of polymers is MicroArray Polymer Profiling (MAPP) (Fangel et al, 2021) formerly known as Comprehensive Microarray Polymer Profiling (CoMPP) (Moller et al, 2007). This method uses a combination of carbohydrate microarrays and molecular probes to directly target the presence of polysaccharides where several monomers assembled in a specific and composition can be identified.…”

Section: Introductionmentioning

confidence: 99%

Exploring the polysaccharide composition of plant cell walls in succulent aloes

Ahl

Pedersen

Jørgensen

et al. 2023

Plants People Planet

View full text Add to dashboard Cite

Societal Impact Statement Aloes are iconic succulent plants native to Africa, Madagascar and the Arabian Peninsula. The succulent leaf mesophyll of aloes has been used extensively as a herbal product for centuries, contributing to their overexploitation. Health benefits are attributed to their polysaccharide content. We present a comprehensive comparison of the polysaccharide composition of succulent tissues from 93 Aloe species. We found polysaccharide composition primarily related to leaf morphology in alignment with the broad range of Aloe species used medicinally. All aloes except Aloe ferox and Aloe vera are endangered raising concern about over‐harvesting of wild species. Summary Aloes are iconic succulent plants native to Africa, Madagascar and the Arabian Peninsula. All aloes except the commercially grown Aloe ferox and Aloe vera are protected according to the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES). Major factors contributing to their over‐exploitation are their ornamental value and medicinal use with more than 25% of Aloe species being utilised. The succulent inner leaf mesophyll of aloes is used in traditional medicine, with the healing effect ascribed to the properties of their structural polysaccharides. To explore the correlation between Aloe polysaccharide profiles and other biologically relevant traits across the genus, we (1) extracted polysaccharides and created profiles for nearly 100 representative species using carbohydrate microarrays and molecular probes. We targeted six major plant cell wall polysaccharide groups using 27 different molecular probes. We (2) tested for phylogenetic signal in the polysaccharide profiles and (3) assembled an exhaustive database from literature on the geographic region, level of endemism, altitude, habitat, habit, medicinal use and leaf morphology of the individual species of Aloe. In the absence of phylogenetic signal of polysaccharide profiles, multivariate linear modelling without phylogenetic correction was used and showed that polysaccharide composition primarily correlated with leaf morphology, highlighting the fundamental role of polysaccharides as the building blocks of plants. No correlations between polysaccharide composition of commercial and non‐commercial species were found. We found polysaccharide composition to primarily relate to leaf morphology emphasising the fundamental and structural role of polysaccharides.

show abstract

Section: Introductionmentioning

confidence: 99%

Exploring the polysaccharide composition of plant cell walls in succulent aloes

Ahl

Pedersen

Jørgensen

et al. 2023

Plants People Planet

View full text Add to dashboard Cite

show abstract

“…To further explore effects of salt treatment on the extensin repeat side-chains Hyp-Ara f 1-4 and overall changes in known polysaccharides, a qualitative survey of cell wall modifications was performed using comprehensive microarray polymer profiling (CoMPP) analysis as described earlier (Moller et al, 2007). Five-day-old Col-0, exad-1 and exad-2 seedlings were transferred to treatments with salt (100 mM NaCl) or control (0 mM NaCl) for 2 days before harvesting for alcohol insoluble residue (AIR) extraction sequentially with CDTA and NaOH fractions, a common method for cell wall polysaccharide analysis (Fangel et al, 2021) (Full dataset in Supplemental Figure S5) . We quantified signal intensities of a selection of monoclonal antibodies (mAbs) specific to glycan epitopes (Supplemental Table S5) .…”

Section: Resultsmentioning

confidence: 99%

“…For polysaccharide epitope assays based on antibodies, different methods of sample preparation affect the extractability and thus may lead to variation in antibody signals (Fangel et al, 2021). Therefore, to test the robustness of JIM11 specificity and characterize further whether salt would affect the JIM11 signal in Col-0, an independent method was performed.…”

Section: Salt Increases Exad-dependent Arabinosylation In Arabidopsis...mentioning

confidence: 99%

Cell wall extensin arabinosylation is required for root directional response to salinity

Zou

Gigli-Bisceglia

Zelm

et al. 2022

Preprint

View full text Add to dashboard Cite

Soil salinity is a major contributor to crop yield losses. To improve our understanding of root responses to salinity, we developed and exploit here a real-time salt-induced tilting assay (SITA). This method follows root growth upon both gravitropic and salt challenges and revealed that root bending is modulated by salinity, but not by osmotic stress. Next, this salt-specific response was measured in 345 natural Arabidopsis accessions and we discovered a genetic locus, encoding for the cell-wall modifying enzyme Extensin Arabinose Deficient arabinosyltransferase (ExAD), to be associated with root bending in salt. Extensins are a class of structural cell wall glycoproteins [hydroxyproline-rich glycoproteins (HRGPs)] which are post-translationally modified by O-glycosylation mostly in the form of hydroxyproline (Hyp)-arabinosylation. Here we show that salt induces ExAD-dependent Hyp-arabinosylation, influencing root bending responses and cell wall thickness. We report that exad mutants, which lack all cell wall Hyp-Araf4 modifications, display increased root epidermal cell wall thickness, and are impaired in the salt-dependent root bending response. Our study thus demonstrates a requirement for extensin Hyp-arabinosylation as a novel cell wall modification for the salt-specific directional response of roots and provides new insight into the process of how plant roots sense and respond to salinity.

show abstract

“…Comprehensive microarray polymer profiling (CoMPP), also called microarray polymer profiling (MAPP) (Fangel et al, 2021), is a profiling method for relative content of different polysaccharides and glycoproteins across multiple samples, which combines the high‐throughput capacity of microarrays with the specificity of molecular probes (Moller et al, 2007; Rydahl et al, 2018). CoMPP allows us to obtain glycomic profiles of numerous samples, yet it is restricted to the fraction of the glycome comprising high‐molecular‐weight carbohydrates (monosaccharides and small oligosaccharides fail to be immobilized on nitrocellulose, see Microarray printing ), most of which correspond to cell wall glycans.…”

Section: Methodsmentioning

confidence: 99%

Cell wall polysaccharide and glycoprotein content tracks growth‐form diversity and an aridity gradient in the leaf‐succulent genus Crassula

Fradera‐Soler,

Mravec,

Harholt

et al. 2023

Physiologia Plantarum

Self Cite

View full text Add to dashboard Cite

Cell wall traits are believed to be a key component of the succulent syndrome, an adaptive syndrome to drought, yet the variability of such traits remains largely unknown. In this study, we surveyed the leaf polysaccharide and glycoprotein composition in a wide sampling of Crassula species that occur naturally along an aridity gradient in southern Africa, and we interpreted its adaptive significance in relation to growth form and arid adaptation. To study the glycomic diversity, we sampled leaf material from 56 Crassula taxa and performed comprehensive microarray polymer profiling to obtain the relative content of cell wall polysaccharides and glycoproteins. This analysis was complemented by the determination of monosaccharide composition and immunolocalization in leaf sections using glycan‐targeting antibodies. We found that compact and non‐compact Crassula species occupy distinct phenotypic spaces in terms of leaf glycomics, particularly in regard to rhamnogalacturonan I, its arabinan side chains, and arabinogalactan proteins (AGPs). Moreover, these cell wall components also correlated positively with increasing aridity, which suggests that they are likely advantageous in terms of arid adaptation. These differences point to compact Crassula species having more elastic cell walls with plasticizing properties, which can be interpreted as an adaptation toward increased drought resistance. Furthermore, we report an intracellular pool of AGPs associated with oil bodies and calcium oxalate crystals, which could be a peculiarity of Crassula and could be linked to increased drought resistance. Our results indicate that glycomics may be underlying arid adaptation and drought resistance in succulent plants.

show abstract

Analytical implications of different methods for preparing plant cell wall material

Cited by 14 publications

References 41 publications

Exploring the polysaccharide composition of plant cell walls in succulent aloes

Exploring the polysaccharide composition of plant cell walls in succulent aloes

Cell wall extensin arabinosylation is required for root directional response to salinity

Cell wall polysaccharide and glycoprotein content tracks growth‐form diversity and an aridity gradient in the leaf‐succulent genus Crassula

Contact Info

Product

Resources

About