Analytical isotachophoresis of lactate in human serum using dry film photoresist microfluidic chips compatible with a commercially available field-deployable instrument platform

Šmejkal, Petr; Breadmore, Michael C.; Guijt, Rosanne M.; Foret, František; Bek, Fritz; Macka, Mirek

doi:10.1016/j.aca.2013.01.046

Cited by 16 publications

(17 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Numerous papers describe the use of a commercial μchip-CE platform (Agilent Bioanalyzer) equipped with a blue LEDIF detector and variously report separation and detection of derivatized carbohydrate oligomers and glycans, as well as microorganisms (171,172). The use of isotachophoresis with indirect LEDIF detection to determine benzoate in sodas and lactate in plasma was also demonstrated (173)(174)(175). Marioka et al (176) used 525-nm LEDIF detection in a chipon-a-compact disc rotating setup following the enzyme-linked immunosorbent assay (ELISA); the authors argue that unlike commercial ELISA instruments, this can be made portable.…”

Section: μChip Scale Fluorescence Detectorsmentioning

confidence: 99%

Light-Emitting Diodes for Analytical Chemistry

Macka

Piasecki

Dasgupta

2014

Annual Rev. Anal. Chem.

Self Cite

114

View full text Add to dashboard Cite

Light-emitting diodes (LEDs) are playing increasingly important roles in analytical chemistry, from the final analysis stage to photoreactors for analyte conversion to actual fabrication of and incorporation in microdevices for analytical use. The extremely fast turn-on/off rates of LEDs have made possible simple approaches to fluorescence lifetime measurement. Although they are increasingly being used as detectors, their wavelength selectivity as detectors has rarely been exploited. From their first proposed use for absorbance measurement in 1970, LEDs have been used in analytical chemistry in too many ways to make a comprehensive review possible. Hence, we critically review here the more recent literature on their use in optical detection and measurement systems. Cloudy as our crystal ball may be, we express our views on the future applications of LEDs in analytical chemistry: The horizon will certainly become wider as LEDs in the deep UV with sufficient intensity become available.

show abstract

Section: μChip Scale Fluorescence Detectorsmentioning

confidence: 99%

Light-Emitting Diodes for Analytical Chemistry

Macka

Piasecki

Dasgupta

2014

Annual Rev. Anal. Chem.

Self Cite

114

View full text Add to dashboard Cite

show abstract

“…Smejkal et al further improved the design using numerical modeling and fabricated the Bioanalyzer-compatible microchip in dry film photoresist (Fig. 4B) [49]. The separation channel comprised wide (300 m, injection and separation) and narrow (30 m, detection) channels.…”

Section: Analytical Itpmentioning

confidence: 99%

Microfluidic isotachophoresis: A review

et al. 2013

Self Cite

View full text Add to dashboard Cite

Electromigration methods including CE and ITP are attractive for incorporation in microfluidic devices because they are relatively easily adaptable to miniaturization. After its popularity in the 1970s, ITP has made a comeback in microfluidic format (μ-ITP, micro-ITP) driven by the advantages of the steady-state boundary, the self-focusing effect, and the ability to aid in preconcentrating analytes in the sample while removing matrix components. In this review, we provide an overview of the developments in the area of μ-ITP in a context of the historic developments with a focus on recent developments in experimental and computational ITP and discuss possible future trends. The chip-ITP areas and topics discussed in this review and the corresponding sections include: PC simulations and modeling, analytical μ-ITP, preconcentration ITP, transient ITP, peak mode ITP, gradient elution ITP, and free-flow ITP, while the conclusions provide a critical summary and outlook. The review also contains experimental conditions for μ-ITP applications to real-world samples from over 50 original journal publications.

show abstract

“…Later on, the accuracy and reproducibility of DNA fragment measurements on the DNA 500 LabChip were assessed by Jabasini et al ( 8 ). Throughout the years, the characteristics of measurements on the Agilent 2100 Bioanalyzer have been published in works presenting its usage in new applications ( 9 - 12 ). …”

Section: Introductionmentioning

confidence: 99%

The characterization of four gene expression analysis in circulating tumor cells made by Multiplex-PCR from the AdnaTest kit on the lab-on-a-chip Agilent DNA 1000 platform

et al. 2016

View full text Add to dashboard Cite

IntroductionNowadays, on-a-chip capillary electrophoresis is a routine method for the detection of PCR fragments. The Agilent 2100 Bioanalyzer was one of the first commercial devices in this field. Our project was designed to study the characteristics of Agilent DNA 1000 kit in PCR fragment analysis as a part of circulating tumour cell (CTC) detection technique. Despite the common use of this kit a complex analysis of the results from a long-term project is still missing.Materials and methodsA commercially available Agilent DNA 1000 kit was used as a final step in the CTC detection (AdnaTest) for the determination of the presence of PCR fragments generated by Multiplex PCR. Data from 30 prostate cancer patients obtained during two years of research were analyzed to determine the trueness and precision of the PCR fragment size determination. Additional experiments were performed to demonstrate the precision (repeatability, reproducibility) and robustness of PCR fragment concentration determination.ResultsThe trueness and precision of the size determination was below 3% and 2% respectively. The repeatability of the concentration determination was below 15%. The difference in concentration determination increases when Multiplex-PCR/storage step is added between the two measurements of one sample.ConclusionsThe characteristics established in our study are in concordance with the manufacturer’s specifications established for a ladder as a sample. However, the concentration determination may vary depending on chip preparation, sample storage and concentration. The 15% variation of concentration determination repeatability was shown to be partly proportional and can be suppressed by proper normalization.

show abstract

Analytical isotachophoresis of lactate in human serum using dry film photoresist microfluidic chips compatible with a commercially available field-deployable instrument platform

Cited by 16 publications

References 28 publications

Light-Emitting Diodes for Analytical Chemistry

Light-Emitting Diodes for Analytical Chemistry

Microfluidic isotachophoresis: A review

The characterization of four gene expression analysis in circulating tumor cells made by Multiplex-PCR from the AdnaTest kit on the lab-on-a-chip Agilent DNA 1000 platform

Contact Info

Product

Resources

About