Analytical Method Development, Validation and Stability Studies by Rp-HPLC Method for Simultaneous Estimation of Andrographolide and Curcumin in Co-Encapsulated Nanostructured Lipid Carrier Drug Delivery System

Abstract

Objective: The current study aims to boost the bioavailability criteria of two natural bioactive compounds, andrographolide and curcumin by their combination in nanostructured lipid carrier (NLC) and also to develop a straightforward reverse-phase high-performance liquid chromatography (RP-HPLC) method to validate, quantify of andrographolide and curcumin simultaneously in novel NLC formulation. Methods: The reliable chromatographic separation was executed by using a column of Phenomenex octadecylsilane (C18) at 35 °C column oven temperature using a mobile phase of 0.02 M potassium dihydrogen orthophosphate (KH2PO4) salt solution of pH 3.0 as a buffer and acetonitrile in 50: 50 v/v fixed ratio and 1.5 ml/min flow rate of with 20 μl injection load. The detection was carried out at 240 nm isosbestic wavelength employing a photodiode array (PDA) detector. Results: Andrographolide and curcumin were eluted at 2.4 and 4.9 min, respectively. Quantification and linearity were achieved for both drugs at the 10-140 μg/ml range. The method is specified as the presence of excipients utilized in the formulation failed to interfere with the estimation of andrographolide and curcumin. The developed method was successfully utilized to work out the drug loading efficiency and in vitro drug release study of those drugs in NLC formulation and also for the estimation of those drugs from rat plasma. Conclusion: The developed high-performance liquid chromatography (HPLC) method may be utilized in the future estimation of andrographolide and curcumin simultaneously in NLC and other nanoformulations both in vitro and in vivo.

Validation, Stability Studies, and Simultaneous Estimation of Co-Encapsulated Curcumin, Epigallocatechin Gallate Nanoformulation by Rp-HPLC Method

“…International Conference on Harmonization (ICH) standards for system suitability, robustness, linearity, specificity, accuracy, precision, the limit of detection (LOD), and the limit of quantification (LOQ) were used to validate the method [28].…”

“…The absence of overlapping peaks in the nanoparticle sample demonstrates the specificity of the method. Furthermore, after various stress degradation studies, the peaks of EGCG and curcumin were clearly distinguished from the degradation products [28].…”

Rp-HPLC Method Development and Validation for Simultaneous Estimation of Curcumin and Resveratrol in Nano-Micelle: Dual Drug Dual Form Simultaneous Estimation

“…The measured peak responses of chromatogram of intra-day and inter-day were statistically calculated for accuracy and precision measurement. Statistically, we have calculated mean, SD, and % RSD [22][23][24][25][26].…”

Nano Carrier Drug Delivery Systems for the Treatment of Cognitive Dysfunction in Depression-an Overview on the Nano Formulations Targeting to the Brain

“…However several other neuroprotective drugs have been evaluated in nanoformulations like curcumin, edaravone and nerve growth factors in neurodegenerative diseases like stroke, epilepsy, parkinsonism, alzheimers disease and brain tumors [52]. Most of these molecules have been successfully nanostructured for therapeutic applications [68]. Gold nanoparticles were shown to protect cognitive impairments, oxidative damage, and inflammation in a rat model of Alzheimer's disease sporadic dementia.…”