Analytical methods for soluble microbial products (SMP) and extracellular polymers (ECP) in wastewater treatment systems: A review

Kunacheva, Chinagarn; Stuckey, David C.

doi:10.1016/j.watres.2014.04.044

Cited by 216 publications

(75 citation statements)

References 93 publications

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“…EPS extraction protocols are based on physical methods (ultrasound, blending or high speed centrifugation, steaming, heating, cation exchange resin or lyophilization) and/or chemical reagents (ethanol, formaldehyde, formamide, NaOH, EDTA or glutaraldehyde) (Adav & Lee, 2008;Azeredo et al, 1998;Brown & Lester, 1980;Chu et al, 2015;Comte et al, 2006;Gong et al, 2009;Kunacheva & Stuckey, 2014;Pan et al, 2010;Tapia et al, 2009). There is no consensus on the best methodology to be used as it depends on biofilm species composition and EPS complexity.…”

Section: Measurement Of Eps Componentsmentioning

confidence: 99%

Critical review on biofilm methods

Azeredo

Azevedo

Briandet

et al. 2016

Critical Reviews in Microbiology

789

679

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Biofilms are widespread in nature and constitute an important strategy implemented by microorganisms to survive in sometimes harsh environmental conditions. They can be beneficial or have a negative impact particularly when formed in industrial settings or on medical devices. As such, research into the formation and elimination of biofilms is important for many disciplines. Several new methodologies have been recently developed for, or adapted to, biofilm studies that have contributed to deeper knowledge on biofilm physiology, structure and composition. In this review, traditional and cutting-edge methods to study biofilm biomass, viability, structure, composition and physiology are addressed. Moreover, as there is a lack of consensus among the diversity of techniques used to grow and study biofilms. This review intends to remedy this, by giving a critical perspective, highlighting the advantages and limitations of several methods. Accordingly, this review aims at helping scientists in finding the most appropriate and up-to-date methods to study their biofilms. ARTICLE HISTORY

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Section: Measurement Of Eps Componentsmentioning

confidence: 99%

Critical review on biofilm methods

Azeredo

Azevedo

Briandet

et al. 2016

Critical Reviews in Microbiology

789

679

View full text Add to dashboard Cite

show abstract

“…Schiener et al (1998) reported that the major component of the soluble organic matter in effluents from biological treatment processes is actually soluble microbial products (SMP). SMP are believed to contain polysaccharides, proteins, humic substances, nucleic acid substances, and other carbohydrates and small molecules (Kunacheva and Stuckey, 2014;Barker and Stuckey, 1999). To release the components of dissolved organic matter (DOM), polysaccharides, proteins and reducing sugar were detected along the height of the reactor, and the results also are shown in Table 1.…”

Section: Nitrate Removal Profile Along the Height Of The Reactormentioning

confidence: 99%

“…It is important to clearly identify the primary organic components of effluents in order to understand the fundamental mechanisms of biological activity that create these compounds, and how to reduce these compounds in the effluent (Kunacheva and Stuckey, 2014). However, the organic components of effluents are still not completely understood in solid carbon sources supporting denitrification system.…”

Section: Introductionmentioning

confidence: 99%

Enhanced removal of nitrate using starch/PCL blends as solid carbon source in a constructed wetland

Shen¹,

Zhou²,

Liu³

et al. 2015

Bioresource Technology

109

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“…The production of extracellular polymeric (EPS) materials and soluble microbial products, which are known to influence membrane fouling, has also been shown to be related to MCRT and HRT [11,12] and may be particularly important where the membrane is directly submerged in the mixed liquor [13]. There is considerable debate in the literature, however, concerning methods for analysing EPS and soluble microbial products, and this lack of consistency makes it difficult to interpret results or make comparisons between different studies [16,17]. At present the strategy appears to be to operate MBRs at long MCRT, since research on aerobic systems has shown that decreasing the MCRT tends to increase the concentration of soluble microbial products and EPS, both of which may have a greater effect on membrane fouling than the concentration of mixed liquor suspended solids (MLSS) alone [11,13,18].…”

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confidence: 99%

Effect of mean cell residence time on transmembrane flux, mixed-liquor characteristics and overall performance of a submerged anaerobic membrane bioreactor

Pacheco-Ruiz

Heaven

Banks

2016

Environmental Technology

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Kinetic control of Mean Cell Residence Time (MCRT) was shown to have a significant impact on membrane flux under steady state conditions. Two laboratory-scale flat-plate submerged anaerobic membrane bioreactors were operated for 245 days on a low-tointermediate strength substrate with high suspended solids. Transmembrane pressure was maintained at 2.2 kPa throughout four experimental phases, while MCRT in one reactor was progressively reduced. This allowed very accurate measurement of sustainable membrane flux rates at different MCRT, and hence the degree of membrane fouling. Performance data were gathered on chemical oxygen demand (COD) removal efficiency, and a COD mass balance was constructed accounting for carbon converted into new biomass and that lost in the effluent as dissolved methane. Measurements of growth yield at each MCRT were made, with physical characterisation of each mixed liquor based on capillary suction time. The results showed membrane flux and MLSS filterability was highest at short MCRT, although specific methane production (SMP) was lower since a proportion of COD removal was accounted for by higher biomass yield. There was no advantage in operating at an MCRT <25 days. When considering the most suitable MCRT there is thus a trade-off between membrane performance, SMP and waste sludge yield.

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Analytical methods for soluble microbial products (SMP) and extracellular polymers (ECP) in wastewater treatment systems: A review

Cited by 216 publications

References 93 publications

Critical review on biofilm methods

Critical review on biofilm methods

Enhanced removal of nitrate using starch/PCL blends as solid carbon source in a constructed wetland

Effect of mean cell residence time on transmembrane flux, mixed-liquor characteristics and overall performance of a submerged anaerobic membrane bioreactor

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