Analytical methods for trace levels of reactive carbonyl compounds formed in lipid peroxidation systems

Shibamoto, Takayuki

doi:10.1016/j.jpba.2006.01.047

Cited by 121 publications

(121 citation statements)

References 153 publications

(151 reference statements)

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“…However, there are some reports that determine these compounds in different biological, environmental or lipid matrices [10][11][12][13][14][15][16][17][18][19][20][21][22][23][24], none of these methods simultaneously determine these LPRRAs in human serum.…”

Section: Analysis Of Serum Samples From Healthy Subjects and Patientsmentioning

confidence: 99%

Analytical method for lipoperoxidation relevant reactive aldehydes in human sera by high-performance liquid chromatography–fluorescence detection

El-Maghrabey

Kishikawa

Ohyama

et al. 2014

Analytical Biochemistry

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A validated, simple and sensitive HPLC method was developed for the simultaneous determination of lipoperoxidation relevant reactive aldehydes; glyoxal (GO), acrolein (ACR), malondialdehyde (MDA) and 4-Hydroxy-2-nonenal (HNE) in human serum. The studied aldehydes were reacted with 2,2'-furil to form fluorescent difurylimidazole derivatives that were separated on respectively, with detection limits ranged from 0.030 to 0.11 nmol/mL. The % RSD of intra-day and inter-day precision didn't exceed 5.0% and 6.2%, respectively, and the accuracy (%found) ranged from 95.5 to 103%. The proposed method was applied for monitoring the four aldehydes in sera of healthy, diabetic and rheumatic human subjects with simple pretreatment steps and without interference from endogenous components. By virtue of its high sensitivity and accuracy, our method enabled detection of differences between analytes concentrations in sera of human subjects at different clinical conditions.

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Section: Analysis Of Serum Samples From Healthy Subjects and Patientsmentioning

confidence: 99%

Analytical method for lipoperoxidation relevant reactive aldehydes in human sera by high-performance liquid chromatography–fluorescence detection

El-Maghrabey

Kishikawa

Ohyama

et al. 2014

Analytical Biochemistry

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“…RCCs may be specifically evaluated, but their chemical reactivity may interfere with sample preparation, necessitating their stabilization as dinitrophenylhydrazine derivatives (11 ). RCC derivatives are further converted into their corresponding hydrazones, which can be separated and analyzed by GC-MS or HPLC (64,65 ).…”

Section: Reactive Intermediate Compoundsmentioning

confidence: 99%

Evaluation of Nonenzymatic Posttranslational Modification–Derived Products as Biomarkers of Molecular Aging of Proteins

2010

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BACKGROUND During their biological life, proteins are exposed in a cumulative fashion to irreversible nonenzymatic, late posttranslational modifications that are responsible for their molecular aging. It is now well established that these damaged proteins constitute a molecular substratum for many dysfunctions described in metabolic and age-related diseases, such as diabetes mellitus, renal insufficiency, atherosclerosis, or neurodegenerative diseases. Accordingly, the specific end products derived from these reactions are considered potentially useful biomarkers for these diseases. CONTENT The aim of this review is to give an overview of nonenzymatic posttranslational modifications of proteins and their influence in vivo, take inventory of the analytical methods available for the measurement of posttranslational modification–derived products, and assess the potential contribution of new technologies for their clinical use as biological markers of protein molecular aging. SUMMARY Despite their clinical relevance, biomarkers of posttranslational modifications of proteins have been studied only in the context of experimental clinical research, owing to the analytical complexity of their measurement. The recent implementation in clinical chemistry laboratories of mass spectrometry–based methods that provide higher specificity and sensitivity has facilitated the measurement of these compounds. These markers are not used currently by clinicians in routine practice, however, and many challenges, such as standardization, have to be confronted before these markers can be used as efficient tools in the detection and monitoring of long-term complications of metabolic and age-related diseases.

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“…The rapid increase in the concentrations of reactive oxygen species (ROS) such as superoxide, hydrogen peroxide, singlet oxygen and the hydroxyl radical may have direct and indirect roles in the stress response, such as acting as an antibiotic agent and controlling gene expression (Kotchoni & Gachomo 2006). Many oxygenated VOCs important in atmospheric chemistry such as formaldehyde, acetone and acetaldehyde are major products of lipid peroxidation by ROS (Dennis & Shibamoto 1990;DeZwart et al 1997;Enoiu et al 2000;Orhan et al 2006;Shibamoto 2006). Although extensively used as biomarkers for lipid oxidation in animals, including humans, their production in stressed plants via this mechanism has not previously been demonstrated.…”

Section: A Summary Of the Dmentioning

confidence: 99%

“…Additional sinks of acetaldehyde are likely to exist in plants due to its high reactivity. For example, acetaldehyde readily forms adducts with proteins, phospholipids and DNA (Niemela et al 1995;Shibamoto 2006). In addition, the presence of acetaldehyde itself in excessive amounts leads to the production of ROS and lipid peroxidation (Zhang et al 1996;Novitskiy et al 2006), which is a major cause of liver damage in alcoholic humans.…”

Section: A Summary Of the Dmentioning

confidence: 99%

Carbon isotope analysis of acetaldehyde emitted from leaves following mechanical stress and anoxia

et al. 2009

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Although the emission of acetaldehyde from plants into the atmosphere following biotic and abiotic stresses may significantly impact air quality and climate, its metabolic origin(s) remains uncertain. We investigated the pathway(s) responsible for the production of acetaldehyde in plants by studying variations in the stable carbon isotope composition of acetaldehyde emitted during leaf anoxia or following mechanical stress. Under an anoxic environment, C3 leaves produced acetaldehyde during ethanolic fermentation with a similar carbon isotopic composition to C3 bulk biomass. In contrast, the initial emission burst following mechanical wounding was 5–12‰ more depleted in 13C than emissions under anoxia. Due to a large kinetic isotope effect during pyruvate decarboxylation catalysed by pyruvate dehydrogenase, acetyl‐CoA and its biosynthetic products such as fatty acids are also depleted in 13C relative to bulk biomass. It is well known that leaf wounding stimulates the release of large quantities of fatty acids from membranes, as well as the accumulation of reactive oxygen species (ROS). We suggest that, following leaf wounding, acetaldehyde depleted in 13C is produced from fatty acid peroxidation reactions initiated by the accumulation of ROS. However, a variety of other pathways could also explain our results, including the conversion of acetyl‐CoA to acetaldehyde by the esterase activity of aldehyde dehydrogenase.

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Analytical methods for trace levels of reactive carbonyl compounds formed in lipid peroxidation systems

Cited by 121 publications

References 153 publications

Analytical method for lipoperoxidation relevant reactive aldehydes in human sera by high-performance liquid chromatography–fluorescence detection

Analytical method for lipoperoxidation relevant reactive aldehydes in human sera by high-performance liquid chromatography–fluorescence detection

Evaluation of Nonenzymatic Posttranslational Modification–Derived Products as Biomarkers of Molecular Aging of Proteins

Carbon isotope analysis of acetaldehyde emitted from leaves following mechanical stress and anoxia

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