Analytical monitoring of alcoholic fermentation using NIR spectroscopy

Abstract

Alcoholic fermentation under Saccharomyces cerevisiae yeasts is governed largely by glucose uptake, biomass formation, ethanol and glycerin production, and acidification. In this work, PLS calibration models were developed with a view to determining these analytical parameters from near infrared spectra and analytical data provided by the corresponding reference methods. The models were applied to a set of samples obtained from various fermentation processes. The glucose, ethanol, and biomass values predicted by the models exhibited a high correlation with those provided by the reference method.

Real time and in situ Near-Infrared Spectroscopy (Nirs) for Quantitative Monitoring of Biomass, Glucose, Ethanol and Glycerine concentrations in an alcoholic fermentation

“…The optimal number of latent (factors) variables was determined by minimizing the sum of residual squared errors of prediction (PRESS), Eq. 1 (Randall, 1999;Blanco et al, 2004;Coffey and Cooley, 1999) and minimizing the root mean squared errors of prediction (Eq. 2) obtained by external validation.…”

“…Analytical spectrometry-based methods have been used to monitor ethanol fermentations (Blanco et al, 2004;Finn et al, 2006;Burratti et al, 2011), as these methods are fast and non-destructive. In addition, these methods allow simultaneous analysis of multiple parameters (Ferreira et al, 1999) without requiring sample pretreatment.…”

“…For at-line monitoring, the sample is transported through an external line to the probe, where the measurement is performed at a sampling rate often inadequate for process control objectives (Blanco et al, 2004;Blanco et al, 2006;Finn et al, 2006;González-Sáiz et al, 2008;Fernandéz-Novales et al, 2008;Xie et al, 2009;Burratti et al, 2001). Grassi et al (2014) used NIRS in off-line mode to monitor pH, biomass and soluble solid content (Brix) in a wort fermentation.…”

Homo- and Hetero-Covariance NMR Spectroscopy and Applications to Process Analytical Technology

“…Spectroscopic Analyses -Developments and Applicationsearlier, and NIR became the standard methodology [66,67]. Later, attempts were made to use Raman spectroscopy [68].…”

Selection of the NIR Region for a Regression Model of the Ethanol Concentration in Fermentation Process by an Online NIR and Mid-IR Dual-Region Spectrometer and 2D Heterospectral Correlation Spectroscopy

“…Fermentation monitoring has been investigated using NIR, mid-IR, and Raman spectroscopy because of its importance in several fields, such as food, pharmaceutical, and biological sciences and technologies. 7,[18][19][20][21][22] In our previous work, ethanol fermentation was monitored with the online NIR/mid-IR dual-region spectrometer, and principal-component analysis revealed that the detection of the end-point in the fermentation can be performed by using either transmission NIR and attenuated total reflection (ATR) mid-IR spectra based on the same chemical changes: relative changes in the concentrations of glucose and ethanol. 7 In the present study, time-dependent NIR/mid-IR spectra of the fermentation solution were acquired, and NIR bands assigned to the CH group were detected from the 2D NIR/mid-IR heterospectral correlation spectrum calculated from the obtained spectra.…”