Analytical performances of the Diazyme ADA assay on the Cobas® 6000 system

Delacour, Hervé; Sauvanet, Christophe; Ceppa, F.; Burnat, P.

doi:10.1016/j.clinbiochem.2010.09.005

Cited by 23 publications

(17 citation statements)

References 7 publications

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“…The ADA levels were estimated using a commercially available kit (Erba, Manheim, Germany) [9]. The assay is based on the enzymatic deamination of adenosine to inosine which is converted to hypoxanthine by purine nucleoside phosphorylase (PNP).…”

Section: Determination Of Adenosine Deaminase (Ada)-mentioning

confidence: 99%

Diagnostic value of serum adenosine deaminase in Type 2 diabetic patients

et al. 2019

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Background: Adenosine deaminase (ADA) is suggested to modulate the bioactivity of insulin, but its clinical significance in Type 2 diabetes mellitus (DM) is not yet established. The present study was undertaken to evaluate serum ADA activity and serum uric acid levels in patients of Type 2 DM. Aim: To evaluate the serum ADA level and to correlate ADA levels with Blood Glucose, Glycated Hemoglobin (HbA 1c) levels in Type-2 DM patients. Material and Method: It is a case control study. The subjects in this study were divided into 3 groups. Group I consisted of 50 normal healthy individuals who served as controls with no history of DM. Group II consisted of 50 patients of Type 2 DM both males & females in the age group of 40-65 years on oral hypoglycemic drugs with HbA 1c <7%. Group III consisted of 50 patients of Type 2 DM both males & females in the age group of 40-65 years on oral hypoglycemic drugs with HbA 1c >7%. Serum levels of fasting blood sugar, HbA 1c , ADA and uric acid were estimated in all the subjects. Results: FBS, HbA 1c , ADA and serum uric acid levels were found to be increased in the patients of Type 2 DM as compared with controls. Conclusion: It is concluded that there is an increase in serum ADA levels with increase in HbA 1c levels. Serum uric acid levels increased with moderately increasing levels of HbA 1c <7% and then decreased with further increasing levels of HbA 1c >7% (a bell-shaped relation).

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Section: Determination Of Adenosine Deaminase (Ada)-mentioning

confidence: 99%

Diagnostic value of serum adenosine deaminase in Type 2 diabetic patients

et al. 2019

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“…First, a cut-off value between 25 and 30 U/L has been previously recommended for TBPE diagnosis (ie, an interval including the cut-off value proposed by Chang et al 1 ) if the Diazyme assay is used on the Cobas 6000 system (Roche Diagnostics, Meylan, France). 2 Second, we have compared the results obtained from using the Diazyme assay on two analytical systems: Cobas 6000 (Roche Diagnostics) and Advia 1800 (Siemens Healthcare Diagnostics, Saint Denis, France) by parallel-testing 79 anonymous pleural fluid samples from different French hospitals. We observed a good correlation between the two analytical systems (r 2 =0.88; t test for correlation coefficient, P<0.001), even though bias was observed (~16 %).…”

Section: Pleural Adenosine Deaminase Cut-off Value For the Diagnosis mentioning

confidence: 99%

Pleural adenosine deaminase cut-off value for the diagnosis of tuberculous pleural effusion using the Diazyme assay

Servonnet¹,

Frédéric²,

Fargeau³

et al. 2018

Hong Kong Med J

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“…The ADA levels were estimated using a commercially available kit (Erba, Manheim, Germany) [11]. The assay is based on the enzymatic deamination of adenosine to inosine which is converted to hypoxanthine by purine nucleoside phosphorylase (PNP).…”

Section: Determination Of Adenosine Deaminase (Ada)mentioning

confidence: 99%

Relation of elevated serum adenosine deaminase levels to glycated hemoglobin and serum uric acid in type 2 diabetes mellitus

et al. 2016

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Background: Adenosine deaminase (ADA) is suggested to be an important enzyme for modulating the bioactivity of insulin, but its clinical significance in Type 2 diabetes mellitus is not yet established. The present study was undertaken to evaluate serum ADA activity and serum uric acid levels in patients of Type 2 DM. It is widely reported that immunological imbalance is one of the key factors associated with the metabolic disturbances in type 2 diabetes mellitus. Aims: The present study aimed to evaluate the serum Adenosine Deaminase level and to correlate ADA levels with Blood Glucose and HbA1c levels in Type-2 Diabetes Mellitus patients. Material and Method: It is a case control study. The subjects included in this study were divided into 3 groups. Group I consisted of 50 normal healthy individuals who served as controls with no history of DM. Group II consisted of 50 patients of Type 2 Diabetes Mellitus both males & females in the age group of 40-65 years on oral hypoglycemic drugs with HbA1 c <7%. Group III consisted of 50 patients of Type 2 Diabetes Mellitus both males & females in the age group of 40-65 years on oral hypoglycemic drugs with HbA1 c >7 %. Serum levels of fasting blood sugar, HbA1 c , ADA and uric acid were estimated in all the subjects under study. Results: All the three parameters, FBS, HbA1 c , ADA and serum uric acid levels were found to be increased in the patients of Type 2 DM as compared to controls. The mean serum uric acid levels showed a bell shaped relation with glycemic control. Conclusion: From the present study, it is concluded that there is an increase in serum ADA levels with increase in HbA1 c levels. It was found that the serum uric acid levels increased with increasing levels of HbA1 c <7% and then decreased with further increasing levels of HbA1 c >7% (a bell-shaped relation).

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Analytical performances of the Diazyme ADA assay on the Cobas® 6000 system

Cited by 23 publications

References 7 publications

Diagnostic value of serum adenosine deaminase in Type 2 diabetic patients

Diagnostic value of serum adenosine deaminase in Type 2 diabetic patients

Pleural adenosine deaminase cut-off value for the diagnosis of tuberculous pleural effusion using the Diazyme assay

Relation of elevated serum adenosine deaminase levels to glycated hemoglobin and serum uric acid in type 2 diabetes mellitus

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