Analytical possibilities for the detection of stanozolol and its metabolites

Confirmatory analysis of 17β-boldenone, 17α-boldenone and androsta-1,4-diene-3,17-dione in bovine urine, faeces, feed and skin swab samples by liquid chromatography–electrospray ionisation tandem mass spectrometry

“…Very recently, it was shown that ␤-Bol could be detected in horse mane samples even up to 12 months following administration using a GC-MS n approach [9]. However, in many cases liquid chromatography-tandem mass spectrometry (LC-MS/MS) can be a robust, easy and sensitive alternative as shown previously for the anabolic steroids trenbolone [10], stanozolol [11][12][13] and nortestosterone [14]. Two multi-analyte LC-MS/MS methods for the detection of anabolic steroids in bovine urine have been reported [15,16].…”

A high-throughput screening (HTS) immunochemical method for the analysis of stanozolol metabolites in cattle urine samples

“…35-70 m SDS. 1 H and 13 C NMR spectra were obtained with a Varian Inova-500 (Varian Inc., Palo Alto, CA) spectrometer (500 MHz for 1 H and 125 MHz for 13 C). Infrared spectra were measured on a Bomem MB120 FT-IR spectrophotometer (Hartmann & Braun, Québec, Canada).…”

“…MgSO 4 , filtered and evaporated to dryness under reduced pressure to obtain 2-hydroxymethyleneandrostan-17b-ol-3-one(2) (1.14 g, 70% yield). 1 …”

“…Stanozolol (17-methyl-2 H-androst-2-eno[3,2-c]-pyrazol-17-ol, St) is one of the most important synthetic anabolic steroids used as growth promoter in cattle, to increase athletic performance and in horseracing [1]. The use of substances having a hormonal action for growth promotion in farm animals was prohibited in 1981 by the European Union (Directive 81/602/EEC).…”

“…Additionally, excretion usually takes place as glucuronide of sulfate conjugates, which complicate the analysis. Many laboratories have validated analytical protocols for the chromatographic detection of the parent compound, however detection of the corresponding metabolites has not always been implemented [1]. Thus, although, immunochemical extraction/determination of, St has been reported using antibodies available from few commercial sources (Abcam, Neogen Corporation, Randox Laboratories), information is limited regarding recognition of the metabolites.…”