Analytical potential of fluorescein analogues for ultrasensitive determinations of phosphorus-containing amino acid herbicides by micellar electrokinetic chromatography with laser-induced fluorescence detection

Molina, Manuel Flores; Silva, Manuel

doi:10.1002/1522-2683(200204)23:7/8<1096::aid-elps1096>3.0.co;2-e

Cited by 54 publications

(16 citation statements)

References 27 publications

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“…However, they could determine only high µg L −1 concentrations of the herbicides of interest and some of them suffered from interference from complex matrices. CE methods with different fluorogenic labels and laser‐induced fluorescence detection could detect sub‐µg L −1 or µg L −1 analytes 19–21. Other methods for glyphosate include linker‐assisted ELISA,9 and an immunosensor validated at 0.05–0.75 µg L −1 , and applicable to water and soil analysis 22…”

Section: Chemical Properties Of Herbicides Of Interestmentioning

confidence: 99%

Ion chromatography/inductively coupled plasma mass spectrometry for simultaneous determination of glyphosate, glufosinate, fosamine and ethephon at nanogram levels in water

Guo

Cai

Yang

2007

Rapid Comm Mass Spectrometry

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This paper describes the first approach that simultaneously quantifies four polar, water-soluble organophosphorus herbicides, i.e., glyphosate, glufosinate, fosamine and ethephon, at nanogram levels in environmental waters. The target herbicides were separated completely by ion chromatography (IC) on a polymer anion-exchange column, Dionex IonPac AS16 (4.0 mm T 250 mm), with 30 mM citric acid flowing at 0.70 mL min S1 as the eluent. On-line inductively coupled plasma mass spectrometry (ICP-MS) using a quadrupole mass spectrometer was employed as a sensitive and selective detector of the effluents. Various parameters affecting the separation and detection were systematically examined and optimized. Detection limits of the herbicides achieved with the proposed IC/ICP-MS method were 1.1-1.4 mg L S1 (as compound) based on a 500-mL sample injection. Matrix anions, metal ions, phosphate, polyphosphates, non-polar and other polar organophosphorus pesticides showed no interference. The developed method was validated using reservoir water, treated water and NEWater samples spiked at the level of 10-25 mg L S1 with satisfactory recoveries (95-109%). It is applicable to the simultaneous determination of mg L S1 concentrations of the herbicides in polluted water. Copyright # 2007 John Wiley & Sons, Ltd.Toxicological risks from human exposure to pesticides may occur through contaminated drinking water and agricultural products. Glyphosate, glufosinate, fosamine and ethephon are widely used organophosphorus herbicides, and their residues have been found in waters, soils, fruits, crops, vegetables, and even in human sera and urine. Currently, these compounds are regulated worldwide. As summarized in Table 1, all four herbicides have similar chemical properties: strong polarity and in most cases, ionic character, high solubility in water, insolubility in organic solvents (except ethephon which is readily soluble in polar organic solvents and slightly soluble in non-polar solvents), and absence of chromophore or fluorophore in their molecular structures. Therefore, it is challenging to determine such herbicides at mg L À1 or even lower levels. It is also of interest to develop sensitive, rapid and simple methods that are capable of simultaneously quantifying them in water.Of the four herbicides, glyphosate and glufosinate are the more commonly monitored at trace residue levels. The methods primarily include gas chromatography (GC), high-performance liquid chromatography (HPLC), capillary electrophoresis (CE), and enzyme-linked immunosorbent assay (ELISA), as discussed in a review. 1 In GC methods, glyphosate and glufosinate, sometimes together with their metabolites, aminomethylphosphonic acid (AMPA) and 3-methylphosphinicopropionic acid (MPPA), are chromatographed after chemical derivatization and detected by flame photometry, electron capture or mass spectrometry (MS). GC/MS methods with sample preconcentration are sensitive with limits of detections (LODs) of less than 1 mg L À1 . [2][3][4] Liquid chromatography methods for g...

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Section: Chemical Properties Of Herbicides Of Interestmentioning

confidence: 99%

Ion chromatography/inductively coupled plasma mass spectrometry for simultaneous determination of glyphosate, glufosinate, fosamine and ethephon at nanogram levels in water

Guo

Cai

Yang

2007

Rapid Comm Mass Spectrometry

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“…The analyte derivatives also migrated closely and did not separate when anionic surfactant, SDS, was used; this seems to have been due to the weak interaction between the negative charges of analyte derivatives with anionic surfactant. On the other hand, neutral surfactants added in BGE have been reported to significantly increase the selectivity in CE separation of fluorescein‐labeled compounds 26. Thus non‐ionic surfactant Brij‐35 ® was investigated for the separation.…”

Section: Resultsmentioning

confidence: 99%

Simultaneous determination of memantine and amantadine in human plasma as fluorescein derivatives by micellar electrokinetic chromatography with laser‐induced fluorescence detection and its clinical application

2010

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A nonionic surfactant MEKC method with LIF detection was developed for the simultaneous determination of memantine, an anti-Alzheimer's disease agent, and amantadine, an anti-Parkinson's disease drug, in human plasma. Before analysis, the plasma samples were pretreated by liquid-liquid extraction with ethyl acetate, and derivatized with 6-carboxyfluorescein N-hydroxysuccinimide ester. The chemical derivatization is performed with 6-carboxyfluorescein N-hydroxysuccinimide ester in ACN - 5 mM pH 9.0 borate buffer (40:60, v/v) at 35 degrees C for 3 h. After the derivatization reaction, hydrodynamic injection (0.5 psi, 8 s) was used to introduce the derivatized solution, and the separation was performed in borate buffer (30 mM, pH 9.5) with the nonionic surfactant Brij-35 (0.07%, w/v); the separation voltage was 6 kV. The linear ranges of the method for the determination of memantine and amantadine in human plasma were over a range of 2.0-60.0 ng/mL. The detection limit was 0.5 ng/mL (S/N=3). This method was applied successfully to monitor the concentration of memantine or amantadine in patients with Alzheimer's disease or Parkinson's disease.

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“…It usually took about 1 h to complete the reaction for DTAF labeling. 20 As for FITC, another frequently used reagent, its isothiocyanate moiety with amino compounds requires much longer reaction times (typically 12 h). 19 In view of the time-consuming derivatization step, great efforts have been made to improve the derivatization efficiency.…”

Section: Introductionmentioning

confidence: 99%

Determination of Herbicides and Its Metabolite in Soil and Water Samples by Capillary Electrophoresis-laser Induced Fluorescence Detection Using Microwave-assisted Derivatization

et al. 2014

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Methods were developed to determine glufosinate (GLUF), glyphosate (GLYP) and its metabolite, aminomethylphosphonic acid (AMPA) by capillary electrophoresis-laser induced fluorescence detection using 5-(4,6-dichlorotriazinylamino) fluorescein (DTAF) and fluorescein isothiocyanate (FITC) as the derivatizing reagents. To accelerate the labeling speed, a microwave-assisted derivatization method was adopted. The derivatizing reaction time was reduced to 180 and 150 s for DTAF and FITC, whose reaction time for conventional labeling was 50 min and 5 h, respectively. The optimum separation conditions for derivatives were as follows: a back ground electrolyte (BGE) of 30 mmol L -1 sodium tetraborate containing 15 mmol L -1 brij-35, hydrodynamic injection 15 s and a 10 kV separation voltage. Under these conditions, the LODs (S/N = 3) for DTAF derivatives were 0.32, 0.19 and 0.15 nmol L -1 for GLUF, GLYP, and AMPA, respectively. The LODs (S/N = 3) for FITC derivatives were 2.60, 3.88 and 2.42 nmol L -1 for GLUF, GLYP, and AMPA, respectively. The applicability of the developed method was demonstrated by the detection of the above herbicides and metabolite in water and soil samples.

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Analytical potential of fluorescein analogues for ultrasensitive determinations of phosphorus-containing amino acid herbicides by micellar electrokinetic chromatography with laser-induced fluorescence detection

Cited by 54 publications

References 27 publications

Ion chromatography/inductively coupled plasma mass spectrometry for simultaneous determination of glyphosate, glufosinate, fosamine and ethephon at nanogram levels in water

Ion chromatography/inductively coupled plasma mass spectrometry for simultaneous determination of glyphosate, glufosinate, fosamine and ethephon at nanogram levels in water

Simultaneous determination of memantine and amantadine in human plasma as fluorescein derivatives by micellar electrokinetic chromatography with laser‐induced fluorescence detection and its clinical application

Determination of Herbicides and Its Metabolite in Soil and Water Samples by Capillary Electrophoresis-laser Induced Fluorescence Detection Using Microwave-assisted Derivatization

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