Analytical procedure for the in-vial derivatization—extraction of phenolic acids and flavonoids in methanolic and aqueous plant extracts followed by gas chromatography with mass-selective detection

Fiamegos, Yiannis C.; Nanos, Christos G.; Vervoort, Jacques; Stalikas, Constantine D.

doi:10.1016/j.chroma.2004.04.041

Cited by 112 publications

(46 citation statements)

References 37 publications

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“…An improved derivatisation procedure using in-vial derivatisation -extraction for the GC -MS analysis of methylated flavonoids and phenolic acids in various herbs has been proposed by Stalikas et al [59,97,98]. Derivatisation takes place under basic conditions so that the hydroxyl groups of the analytes are deprotonated.…”

Section: Gas Chromatographymentioning

confidence: 99%

“…In the literature, reported extraction times vary up to 12 h using this extraction mode. Various flavonoids were extracted from Tilia europea, Urtica dioica, Mentha spicata, and Hypericum perforatum after 12 h Soxhlet extraction with methanol [59]. Also, phenolic acids were quantitatively obtained by the same extraction technique from the aerial parts of Echinacea purpurea [60].…”

Section: Extractionmentioning

confidence: 99%

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Extraction, separation, and detection methods for phenolic acids and flavonoids

Stalikas¹

2007

J of Separation Science

850

539

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ReviewExtraction, separation, and detection methods for phenolic acids and flavonoidsThe impetus for developing analytical methods for phenolic compounds in natural products has proved to be multifaceted. Hundreds of publications on the analysis of this category of compounds have appeared over the past two decades. Traditional and more advanced techniques have come to prominence for sample preparation, separation, detection, and identification. This review provides an updated and extensive overview of methods and their applications in natural product matrices and samples of biological origin. In addition, it critically appraises recent developments and trends, and provides selected representative bibliographic examples.

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Section: Gas Chromatographymentioning

confidence: 99%

Section: Extractionmentioning

confidence: 99%

Extraction, separation, and detection methods for phenolic acids and flavonoids

Stalikas¹

2007

J of Separation Science

850

539

View full text Add to dashboard Cite

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“…Depending on the eluent pH, the ferulic and p-coumaric acids present double peaks at higher pH values. This can be explained by the presence of weak organic acids, with pKa values of around 4.5 and 9.5 [17]. The best results were obtained with the mobile phase adjusted to pH 2.08 with phosphoric acid (for all three solvents) because ionization of the phenolic acids is suppressed at this pH value.…”

Section: Development Of the Hplc Methodsmentioning

confidence: 50%

“…Phenolic compounds have been analyzed in different samples by capillary electrophoresis (CE) [12][13][14][15], gas chromatography equipped with a mass spectrometric detector (GC-MS) [16][17][18][19][20], ultra performance liquid chromatography (UPLC) [21] and high performance liquid chromatography (HPLC) [3,[5][6][7]9,[20][21][22][23][24][25][26][27]. HPLC is most frequently used because it does not require a derivatization sample for analysis as gas chromatography [24].…”

Section: Introductionmentioning

confidence: 99%

Use of Ultrasound Bath in the Extraction and Quantification of Ester-Linked Phenolic Acids in Tropical Forages

Santos¹,

Vitor²,

Carneiro³

et al. 2011

AJAC

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A method was developed for the analysis of ester-linked phenolic acids in forage samples using extraction by an ultrasound-assisted treatment and quantification by HPLC with a UV-VIS detector. A reversed-phase C18 column was used for developing the method and the optimal condition was established with isocratic elution using acetonitrile/methanol/H 3 PO 4 pH 2.08 (13:12.5:74.5) as the mobile phase. To reduce the time of sample processing, the extraction of ester-linked phenolic acids was studied using ultrasound bath and the results were then compared with those from an extraction usual using alkaline hydrolysis (20˚C for 24 h). The method was valued through external and internal calibration. Internal calibration using o-coumaric acid as internal standard and m-coumaric acid as surrogate internal standard showed better results. The detection limits were of 0.09 and 0.04 mg·L -1 for p-coumaric and ferulic acids, respectively. The proposed method showed a good linear dynamic range (3.00 -30.00 mg·L -1 ) for the analytes. The usefulness of the method-ology was demonstrated by addition-recovery experiments using forage samples and values were in the 83 to 99% range. The extraction of ester-linked phenolic acids by 120 minutes of ultrasound bath was faster and more reproducible than alkaline hydrolysis (20˚C for 24 h).

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“…It has been reported that iridoid glycosides and flavonoids are two major components of L. rotata [3][4][5]. Many HPLC [6][7][8][9], CE [10][11][12][13][14], highperformance liquid chromatographic-mass spectrometric (HPLC-MS) [15][16][17], and gas chromatographic-mass spectrometric (GC-MS) [18,19] methods have been developed for the separation and analysis of iridoid glycosides and flavonoids. 8-O-acetylshanzhiside methylester, 6-O-acetylshanzhiside methylester, shanzhiside methylester, 8-deoxyshanzhiside, sesamoside, loganin, penstemoside, phlomiol, 7, 8-dehydropenstemoside, phloyoside I, phloyoside II, lamiophlomiside, phlorigidoside C, and seven other iridoid diglycosides have been isolated and reported as iridoid glycosides in the last 20 years [20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Separation and analysis of phenylethanoid glycosides inLamiophlomis rotataby high-performance liquid chromatography

et al. 2013

Acta Chromatographica

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Summary.A simple hydrolysis method has been developed for determination of phenylethanoid glycosides in Lamiophlomis rotata (L.R.). Different kinds of phenylethanoid glycosides were hydrolyzed in hydrochloric acid solution to produce corresponding phenethyl alcohols and cinnamic acids, mainly containing hydroxytyrosol, homovanillyl alcohol, 3,4-dimethoxyphenethyl alcohol, caffeic acid, fumalic acid and 3,4-dimethoxycinnamic acid. The six analytes could be determined simultaneously by high-performance liquid chromatography (HPLC). The effects of mobile phase, pH and concentration of running buffer, detection wavelength, flow rate and injection volume were also investigated. Under the optimum conditions, the six hydrolyzates could be perfectly separated within 45 min. The response was linear over four orders of magnitude with detection limits (S/N ＝ 3) ranging from 1 × 10 −8 to 1.5 × 10 −4 mol L −1 for the analytes. The method has been successfully applied to the analysis of real sample Du-Yi-Wei capsule and Qi-Zheng-Yan-Tong patch, with satisfactory results.

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Analytical procedure for the in-vial derivatization—extraction of phenolic acids and flavonoids in methanolic and aqueous plant extracts followed by gas chromatography with mass-selective detection

Cited by 112 publications

References 37 publications

Extraction, separation, and detection methods for phenolic acids and flavonoids

Extraction, separation, and detection methods for phenolic acids and flavonoids

Use of Ultrasound Bath in the Extraction and Quantification of Ester-Linked Phenolic Acids in Tropical Forages

Separation and analysis of phenylethanoid glycosides inLamiophlomis rotataby high-performance liquid chromatography

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