Analytical radiography for planar radiographic images implemented with a multi-modal system

Vizard, Douglas L.; Wood, Douglas; Papineni, Rao; Feke, Gilbert D.; Orton, Sean; McLaughlin, William E.

doi:10.1016/j.cmpb.2010.01.003

Cited by 5 publications

(6 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Binning was set at 2 X & 2 Y with a 0.4 mm aluminium filter, an ƒ-stop of 5.57, an exposure time of 2 min, and FOV = 200, which provided sufficient grey scale values for acquiring an image quality suitable for bone mineral density measurements. Images were processed using Bruker Molecular Imaging software v.7.1.3.20550 with the Bruker Bone Density Software Module by quantifying pixels after selecting the femur regions of interest [40,41].…”

Section: Methodsmentioning

confidence: 99%

Calcitriol Analogues Decrease Lung Metastasis but Impair Bone Metabolism in Aged Ovariectomized Mice Bearing 4T1 Mammary Gland Tumours

Anisiewicz¹,

Filip‐Psurska²,

Pawlik³

et al. 2019

Aging and disease

View full text Add to dashboard Cite

Calcitriol and its analogues are considered drugs supporting the anticancer treatment of breast cancer and preventing the osteoporosis that results from the development of cancer or from chemotherapy or hormone therapy. Following the orthotopic implantation of 4T1 mammary carcinoma cells into aged ovariectomized (OVX) mice, we evaluated the effects of calcitriol and its two analogues, PRI-2191 and PRI-2205, on metastatic spread and bone homeostasis. Calcitriol and its analogues temporarily inhibited the formation of metastases in the lungs. Unexpectedly, only mice treated with calcitriol analogues showed a deterioration of bone-related parameters, such as bone column density, marrow column density and the CaPO4 coefficient. These findings correlated with an increased number of active osteoclasts differentiated from bone marrow-derived macrophages in mice treated with the analogues. Interestingly, in the tumours from mice treated with PRI-2191 and PRI-2205, the expression of Tnfsf11 (RANKL) was increased. On the other hand, osteopontin (OPN) levels in plasma and tumour tissue, as well as TRAC5b levels in tumours, were diminished by calcitriol and its analogues. Despite a similar action of both analogues towards bone metabolism, their impact on vitamin D metabolism differed. In particular, PRI-2191 and calcitriol, not PRI-2205 treatment significantly diminished the levels of both 25(OH)D3 and 24,25(OH)2D3. In conclusion, though there is evident antimetastatic activity in old OVX mice, signs of increased bone metabolism and deterioration of bone mineralization during therapy with calcitriol analogues were observed.

show abstract

Section: Methodsmentioning

confidence: 99%

Calcitriol Analogues Decrease Lung Metastasis but Impair Bone Metabolism in Aged Ovariectomized Mice Bearing 4T1 Mammary Gland Tumours

Anisiewicz¹,

Filip‐Psurska²,

Pawlik³

et al. 2019

Aging and disease

View full text Add to dashboard Cite

show abstract

“…The exact location was defined from the Chi-square value, derived from the bone density analysis, which is a measure of the fitting quality in the non-linear least-squares fit of the cylinder to the bone segment. The ROI was analyzed for changes in long-bone density (g/cm 3 ) using Carestream Molecular Imaging Software (version 5.3.3) with bone density software module ( 35 ). In addition, a ROI was drawn around any calcification within the Achilles to measure its area (pixels) and a linear ROI used to measure the height and width of the tibia at the tibio-fibular joint (millimeter) (Figure S1 in Supplementary Material).…”

Section: Methodsmentioning

confidence: 99%

Deletion of P58IPK, the Cellular Inhibitor of the Protein Kinases PKR and PERK, Causes Bone Changes and Joint Degeneration in Mice

et al. 2014

View full text Add to dashboard Cite

Objective: Protein kinase-like endoplasmic reticulum kinase (PERK) and protein kinase R (PKR) are implicated in endoplasmic reticulum stress-induced arthritis and pro-inflammatory cytokine-mediated cartilage degradation in vitro, respectively. We determined whether knockout of the cellular inhibitor of PERK and PKR, P58IPK causes joint degeneration in vivo and whether these molecules are activated in human osteoarthritis (OA).Materials and Methods: Sections of knee joints from P58IPK-null and wild-type mice aged 12–13 and 23–25 months were stained with toluidine blue and scored for degeneration using the osteoarthritis research society international (OARSI) system. Bone changes were assessed by radiology and high-resolution micro-computed tomography of hind limbs. Sections from the medial tibial plateaus of two human knees, removed in total knee replacement surgery for OA, were immunolabelled for phosphorylated PERK and PKR and P58IPK.Results: Knockout mice exhibited narrower tibiae (p = 0.0031) and smaller epiphyses in tibiae (p = 0.0004) and femora (p = 0.0214). Older knockout mice had reduced total volume inside the femoral periosteal envelope (p = 0.023), reduced tibial (p = 0.03), and femoral (p = 0.0012) bone volumes (BV) and reduced femoral BV fraction (p = 0.025). Compared with wild-types, younger P58IPK-null mice had increased OARSI scores in medial femoral condyles (p = 0.035). Thirty four percent of null mice displayed severe joint degeneration with complete articular cartilage loss from the medial compartment and heterotopic chondro-osseous tissue in the medial joint capsule. Phosphorylated PERK and PKR were localized throughout human osteoarthritic tibial plateaus but, in particular, in areas exhibiting the most degeneration. There was limited expression of P58IPK.Conclusion: This study is the first to reveal a critical role for P58IPK in maintaining joint integrity in vivo, implicating the PKR and PERK stress signaling pathways in bony changes underlying the pathogenesis of joint degeneration.

show abstract

“…Bone density and radius of X-rayed femurs was measured using Bruker Molecular Imaging Software v. 7.2.0.21122 with the Bruker Bone Density Software Module. Briefly, the Bone Density Software Module estimates long-bone mineralisation by modelling a cylindrical symmetry to an X-ray image and extracting the relevant parameters (Vizard et al, 2010).…”

Section: Bone Density Analysismentioning

confidence: 99%

The Role of Microrna-29 in Osteoarthritis

Smith

Clark

2022

Osteoarthritis and Cartilage

View full text Add to dashboard Cite

Analytical radiography for planar radiographic images implemented with a multi-modal system

Cited by 5 publications

References 8 publications

Calcitriol Analogues Decrease Lung Metastasis but Impair Bone Metabolism in Aged Ovariectomized Mice Bearing 4T1 Mammary Gland Tumours

Calcitriol Analogues Decrease Lung Metastasis but Impair Bone Metabolism in Aged Ovariectomized Mice Bearing 4T1 Mammary Gland Tumours

Deletion of P58IPK, the Cellular Inhibitor of the Protein Kinases PKR and PERK, Causes Bone Changes and Joint Degeneration in Mice

The Role of Microrna-29 in Osteoarthritis

Contact Info

Product

Resources

About