Analytical Review on Raloxifene -An Estrogen Receptor Modulator in Different Pharmaceutical Formulations and Biological Fluids

Aim: A newer LC–MS/MS method was developed and validated for the simultaneous quantification of raloxifene (RL) and cladrin (CL). Methodology: Both drugs were resolved in RP-18 (4.6 × 50 mm, 5 μ) Xbridge Shield column using acetonitrile and 0.1% aqueous solution of formic acid (FA) (70:30% v/v) as mobile phase by using biological matrices in female Sprague–Dawley rats using–MS/MS. Results: The developed method was found to be linear over the concentration ranges of 1–600 ng/ml, and lower limit of quantification was 1 ng/ml for RL and CL, respectively. Pharmacokinetic results of RL+CL showed Cmax = 4.23 ± 0.61, 26.97 ± 1.14 ng/ml, at Tmax(h) 5.5 ± 1.00 and 3.5 ± 1.00, respectively. Conclusion: Pharmacokinetic study results will be useful in the future for the combined delivery of RL and CL for osteoporosis treatment.

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A new Validated RP-HPLC Method for simultaneous quantification of Raloxifene and Curcumin

Tagde¹,

Nagpal²,

Kulkarni³

2022

RJPT

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Raloxifene (RLX) and Curcumin (CUR) are used for the treatment of breast cancer. Its combination is chosen because of their synergistic therapeutic potential, which helps to reduce drug-related toxicities while increasing therapeutic efficacy. A simple, sensitive, accurate and specific technique for determining retention time by employing reverse-phase high-performance liquid chromatography for simultaneous estimation of both the drugs was developed as well validated for the analysis on their particular wavelength. The separation was performed on an RP C-18 Column (EC 250/4 neucleosil 100-10) with column dimensions 250mm x 4.60mm, 10mM using mobile phase Potassium dihydrogen phosphate: acetonitrile (pH 3.0 with Orthophosphoric acid) in the ratio of 20:80 v/v and flow rate of 1.0min/mL. The specificity, linearity range, accuracy, sensitivity, robustness, precision, and ruggedness of the method were all determined. The analyte's peaks were found to be well resolved, and two different RTs for RLX and CUR were observed. Over a concentration range of 1-5g/ml, RLX and CUR calibration curves were shown to be linear. All of the technique validation criteria were within acceptable limits. For inter- and intra-day precision, the relative standard deviation (%RSD) was found to be <2%.

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Analytical Review on Raloxifene -An Estrogen Receptor Modulator in Different Pharmaceutical Formulations and Biological Fluids

Cited by 3 publications

References 28 publications

Study of micelle and metal complexation-mediated fluorescence enhancements of raloxifene hydrochloride. Application to pharmaceutical analysis

Study of micelle and metal complexation-mediated fluorescence enhancements of raloxifene hydrochloride. Application to pharmaceutical analysis

LC–MS/MS Method for Simultaneous Estimation of Raloxifene, Cladrin in rat Plasma: Application in Pharmacokinetic Studies

A new Validated RP-HPLC Method for simultaneous quantification of Raloxifene and Curcumin

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