Analytical sensitivity of COVID-19 rapid antigen tests: A case for a robust reference standard

Toft, Casey J.; Bourquin, Rebecca A.; Sorenson, Alanna E.; Horwood, Paul F.; Druce, Julian; Schaeffer, Patrick M.

doi:10.1016/j.talo.2023.100187

Cited by 7 publications

(2 citation statements)

References 37 publications

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“…Manufacturers report LODs ranging from tens to hundreds of TCID50/mL of virus although there are reports suggesting these sensitivities are much lower than the true values. [ 104 ] SERS‐based RAT tests have recently been reported and these approaches have increased the sensitivity by a factor of about 100. [ 105 ] ELISA approaches have also been developed with LODs of about 100 PFU/mL which is about 30 times less sensitive than the SERS approaches developed in the same lab.…”

Section: Gold Nanoparticles In Sars‐cov‐2 Detectionmentioning

confidence: 99%

A review of metal nanoparticle‐based surface‐enhanced Raman scattering substrates for severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) detection

et al. 2023

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Monitoring an infectious disease early using highly sensitive and non‐invasive techniques is critical for human health. Interestingly, the development of surface‐enhanced Raman scattering (SERS) for biological detection ideally fits these medical requirements and is rapidly growing as a powerful diagnostic tool. SERS can enhance the Raman signal of the target molecule by more than 106 after the adsorption of the molecule on the plasmonic nanostructured surface. This review provides an overview of the use of gold and silver nanoparticles in SERS substrate designs, followed by the development of these SERS substrates in severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) detection.

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Section: Gold Nanoparticles In Sars‐cov‐2 Detectionmentioning

confidence: 99%

A review of metal nanoparticle‐based surface‐enhanced Raman scattering substrates for severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) detection

et al. 2023

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“…This has raised concerns about potential reductions in sensitivity compared to other variants. The FDA has indicated that RATs may have reduced sensitivity in detecting the Omicron variant, and studies assessing the reliability of RATs for Omicron detection have produced conflicting results, possibly due to the presence of undiagnosed Omicron-infected patients [15][16][17][18][19][20][21].…”

Section: Introductionmentioning

confidence: 99%

The Suitability of RNA from Positive SARS-CoV-2 Rapid Antigen Tests for Whole Virus Genome Sequencing and Variant Identification to Maintain Genomic Surveillance

Cucina,

Contino,

Brunacci

et al. 2023

Diagnostics

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The COVID-19 pandemic has transformed laboratory management, with a surge in demand for diagnostic tests prompting the adoption of new diagnostic assays and the spread of variant surveillance tools. Rapid antigen tests (RATs) were initially used only for screening and later as suitable infection assessment tools. This study explores the feasibility of sequencing the SARS-CoV-2 genome from the residue of the nasopharyngeal swab extraction buffers of rapid antigen tests (RATs) to identify different COVID-19 lineages and sub-lineages. Methods: Viral RNA was extracted from the residue of the nasopharyngeal swab extraction buffers of RATs and, after a confirmation of positivity through a reaction of RT-PCR, viral genome sequencing was performed. Results: Overall, the quality of the sequences obtained from the RNA extracted from the residue of the nasopharyngeal swab extraction buffers of RATs was adequate and allowed us to identify the SARS-CoV-2 variants’ circulation and distribution in a period when the use of molecular swabs had been drastically reduced. Conclusions: This study demonstrates the potential for genomic surveillance by sequencing SARS-CoV-2 from the residue of the nasopharyngeal swab extraction buffers of RATs, highlighting alternative possibilities for tracking variants.

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Chest X-ray Images for Lung Disease Detection Using Deep Learning Techniques: A Comprehensive Survey

Al-qaness,

Zhu,

AL-Alimi

et al. 2024

Arch Computat Methods Eng

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Analytical sensitivity of COVID-19 rapid antigen tests: A case for a robust reference standard

Cited by 7 publications

References 37 publications

A review of metal nanoparticle‐based surface‐enhanced Raman scattering substrates for severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) detection

A review of metal nanoparticle‐based surface‐enhanced Raman scattering substrates for severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) detection

The Suitability of RNA from Positive SARS-CoV-2 Rapid Antigen Tests for Whole Virus Genome Sequencing and Variant Identification to Maintain Genomic Surveillance

Chest X-ray Images for Lung Disease Detection Using Deep Learning Techniques: A Comprehensive Survey

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