Analytical separation of tea catechins and food-related polyphenols by high-speed counter-current chromatography

Yanagida, Akio; Shoji, Atsushi; Shibusawa, Yöichi; Shindo, Heisaburo; Tagashira, Motoyuki; Ikeda, Mitsuo; Ito, Yoichiro

doi:10.1016/j.chroma.2005.09.086

Cited by 94 publications

(46 citation statements)

References 31 publications

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“…depend on the degree of their polymerization, which makes it necessary to establish a reliable separation method. NP, RP, and size-exclusion LC tend to cause irreversible adsorption of procyanidins onto the column packing materials, whereas no losses were observed with the CCC separation [74].…”

Section: High-speed Hydrodynamic Centrifuges (Hsccc)mentioning

confidence: 99%

Countercurrent Chromatography in Analytical Chemistry (IUPAC Technical Report)

2009

Chemistry International -- Newsmagazine for IUPAC

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Section: High-speed Hydrodynamic Centrifuges (Hsccc)mentioning

confidence: 99%

Countercurrent Chromatography in Analytical Chemistry (IUPAC Technical Report)

2009

Chemistry International -- Newsmagazine for IUPAC

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“…Recently, numbers of studies have been performed in order to investigate the relationship between green tea consumption and health [2][3][4]. For example, recent evidence suggests that there is an inverse relationship between tea consumption and ischemic heart disease [5]. In addition, there have been many reports referring to other biological functions of tea, such as anti-inammation, anti-oxidation, anti-allergy and anti-obesity [6,7].…”

Section: Introductionmentioning

confidence: 98%

Microwave‐assisted extraction followed by CE for determination of catechin and epicatechin in green tea

Huang

Zhuxin³

et al. 2010

J of Separation Science

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In this work, for the first time, microwave-assisted extraction (MAE) followed by CE was developed for the fast analysis of catechin and epicatechin in green tea. In the proposed method, catechin and epicatechin in green tea samples were rapidly extracted by MAE technique, and then analyzed by CE. The MAE conditions and the method's validation were studied. It is found that the extraction time of 1 min with 400 W microwave irradiation is enough to completely extract catechin and epicatechin in green tea sample, whereas the conventional ultrasonic extraction (USE) technique needs long extraction time of 60 min. The method validations were also studied in this work. The calibration curve shows good linearity in 0.01-3 mg/mL for catechin (R(2)=0.993), and 0.005-3 mg/mL for epicatechin (R(2)=0.996), respectively. The RSD values for catechin and epicatechin are 0.65 and 2.58%, respectively. This shows that the proposed method has good reproducibility. The proposed method has good recoveries, which are 118% for catechin and 120% for epicatechin. The proposed method was successfully applied to determination of the catechin and epicatechin in different green tea samples. The experiment results have demonstrated that the MAE following CE is a simple, fast and reliable method for the determination of catechin and epicatechin in green tea.

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“…The upper and lower phases were determined at 280 nm. The K-values were calculated according to the following formula: K = As/Am [18]. The K-values of six selected solvent systems were presented in Table 1.…”

Section: Solvent System For Hscccmentioning

confidence: 99%

“…According to references [11][12][13][14][15][16][17] and the preliminary results, the solvent system was adjusted on the basis of hexane/ethyl acetate/methanol/water and hexane/ethyl acetate/water. The K-values were determined by uv-spectrophotometer [18]. An amount of sample was added to a 10 ml test tube with 4 ml of each phase of the two phase solvent system.…”

Section: Solvent System For Hscccmentioning

confidence: 99%

Preparative isolation of monomer catechins and oligomer procyanidin fractions from grape seed extracts by high-speed counter-current chromatography

Zhang

Cui

Sun

2014

BIO Web of Conferences

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Abstract. In this paper, monomer (+)-catechin; (-)-epicatechin and (-)-epicatechin-3-o-gallate, three oligomeric procyanidin fractions (OPCs) and one polymeric procyanidin fractions (PPCs) were successfully separated and isolated from grape seed extracts using semi-preparative TBE-300 B high-speed counter-current chromatography (HSCCC). For their separation in monomer (+)-catechin; (-)-epicatechin and (-)-epicatechin 3-O-gallate, hexane/ethyl acetate/water (1:10: 10, v/v/v) was selected as solvent system. For separation of OPCs and PPCs, solvent system ethyl acetate/water (1:1, v/v) was selected. Compositional data and mean polymerization degrees of OPCs were determined by phloroglucinolysis method. The purity of each fraction obtained; monomer (+)-catechin (C), (-)-epicatechin (EC) and (-)-epicatechin-3-o-gallate (ECG), verified by UPLC, was 98%, 82% and 71%, respectively. The mean polymerization degrees of the three OPCs fractions were respectively 2.842, 3.521, 4.475. As compared with traditional separation methods for catechins and OPCs, HSCCC has shown to be a powerful and efficient technique for large-scale isolation of these compounds.

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Analytical separation of tea catechins and food-related polyphenols by high-speed counter-current chromatography

Cited by 94 publications

References 31 publications

Countercurrent Chromatography in Analytical Chemistry (IUPAC Technical Report)

Countercurrent Chromatography in Analytical Chemistry (IUPAC Technical Report)

Microwave‐assisted extraction followed by CE for determination of catechin and epicatechin in green tea

Preparative isolation of monomer catechins and oligomer procyanidin fractions from grape seed extracts by high-speed counter-current chromatography

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