Analytical strategies for arsenic speciation in environmental and biological samples

Feldmann, Jörg; Devalla, S; Raab, Andrea; Hansen, Hanna E.

doi:10.1007/978-3-662-09135-7_3

Cited by 9 publications

(7 citation statements)

References 35 publications

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“…The speciation analysis by using the combination of the three-hour hot base digestion with HG-GC-AAS technique revealed that majority of the arsenic species present in both the control and the main decomposition samples were in the form of "hidden" arsenic compounds [25,26]. Studies have shown that the majority of these hidden organoarsenic compounds in marine algae are arsenosugars [27].…”

Section: Resultsmentioning

confidence: 99%

Closed Anaerobic Biotransformation Products of Organoarsenic Compounds inFucus distichus

Ojo

Onasanya

2013

ISRN Environmental Chemistry

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The closed anaerobic decomposition extracts of Fucus distichus incubated with seawater and sediment, and without sediment as control, were subjected to extractions and isolation on Sephadex LH 20 and Cellulose Thin Layer Chromatography. The decomposition extracts and isolates were analyzed by using both the Hydride Generation Gas Chromatography Atomic Absorption Spectrometry (HG-GC-AAS) and High Performance Liquid Chromatography Inductively Coupled Plasma Mass Spectrometry (HPLC-ICPMS) to identify the arsenic species in the equilibrium mixtures of the seaweed and filtrates separately. In the methanol seaweed extract, equilibrium mixture of arsenosugars (AS) AS1 and AS2 and their biotransformation products of dimethylarsinoylethanol (DMAE) and dimethylarsinic acid (DMAA) were identified. In the methanol filtrate extract of the mixture, only DMAE and DMAA were identified. However, in the control methanol filtrate extract five organoarsenic species, AS1 and AS2, one unidentified hidden organoarsenic species, DMAE and DMAA were identified in the equilibrium mixture. This result confirmed that the hidden organoarsenic species in Fucus distichus, AS1 and AS2, and an unidentified organoarsenic compounds are biotransformed to only DMAE and DMAA under an anaerobic condition. This also suggests that DMAE and DMAA are strong intermediate candidates for the generation of arsenobetaine, from arsenoribosides in the marine food webs.

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Section: Resultsmentioning

confidence: 99%

Closed Anaerobic Biotransformation Products of Organoarsenic Compounds inFucus distichus

Ojo

Onasanya

2013

ISRN Environmental Chemistry

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“…Three arsenosugars (sugar-1, sugar-2 and sugar-3) were identified in the seaweed extract by comparing the retention times with purified arsenosugars. The occurrence of these arsenosugars was confirmed in a later study using carbonate buffer and the parallel use of electrospray-MS and ICP-MS. 20 L. digitata contains sugar-3 (71.9%) as major extractable arsenic species (Fig. 3), in addition to the two minor arsenosugars sugar-2 (18.2%) and sugar-1 (7.6%).…”

Section: Speciation Analysis and Environmentmentioning

confidence: 99%

Biodegradation of arsenosugars in marine sediment

Pengprecha

Wilson

Raab

et al. 2005

Appl. Organometal. Chem.

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In the marine environment, arsenic accumulates in seaweed and occurs mostly in the form of arsenoribofuranosides (often called arsenosugars). This study investigated the degradation pathways of arsenosugars from decaying seaweed in a mesocosm experiment. Brown seaweed (Laminaria digitata) was placed on top of a marine sediment soaked with seawater. Seawater and porewater samples from different depths were collected and analysed for arsenic species in order to identify the degradation products using high-performance liquid chomatography-inductively coupled plasma mass spectrometry. During the first 10 days most of the arsenic found in the seawater and the shallow sediment is in the form of the arsenosugars released from the seaweed. Dimethylarsenoylethanol (DMAE), dimethylarsinic acid (DMA(V)) and, later, monomethylarsonic acid (MMA(V)) and arsenite and arsenate were also formed. In the deeper anaerobic sediment, the arsenosugars disappear more quickly and DMAE is the main metabolite with 60-80% of the total arsenic for the first 60 days besides a constant DMA(V) contribution of 10-20% of total soluble arsenic. With the degradation of the soluble DMAE the solubility of arsenic decreases in the sediment. The final soluble degradation products (after 106 days) were arsenite, arsenate, MMA(V) and DMA(V). No arsenobetaine or arsenocholine were identified in the porewater.

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“…A recently developed CEX gradient separation (0-20 mM + NH 4 , pH = 2.5 [77]) on a PRP-X200 column improved the separation somewhat, but again confirmed the prob- Therefore AEX is recommended [13,134] as the primary separation and, if required, CEX as a secondary option to separate the less toxic organic cationic arsenicals not resolved on an anion exchanger. This can be achieved by a dual mode method, which consists on one hand of an additionally applied CEX procedure (as two columns two procedures [13] or as two columns in series in one procedure [135,136]). On the other hand, a mixed mode ion exchanger (mostly AS7 column,) can be used, containing both, anionic and cationic exchange sites [127].…”

Section: Selective Separation Methodsmentioning

confidence: 99%

“…The method should be robust in routine speciation analysis, provide lowest DL, and separate as many as possible of diverse As-species. Strategies for arsenic speciation analysis have been presented by Larsen [126] and Feldmann et al [13].…”

Section: Selective Separation Methodsmentioning

confidence: 99%

“…Inorganic redox species arsenite (AsIII) and arsenate (AsV) are far the most abundant and the most toxic species in environmental waters. In biologically mediated transformations these species are converted into numerous organic As-species [13,14]. All As-related problems like toxicity, adsorption and transport, biogeochemical cycling and treatment of drinking water depend on As speciation.…”

Section: Introductionmentioning

confidence: 99%

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Arsenic Speciation Analysis by Ion Chromatography - A Critical Review of Principles and Applications

Ammann¹

2011

AJAC

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Multiple acute and chronic toxicity of arsenic species and its mobilisation from geological deposits into ground and drinking water resources are one of the greatest threats to human health. Arsenic speciation analysis, mostly done by liquid chromatography, is a challenging task which requires an intense high quality work with respect to extraction, preservation, separation, detection and validation. A growing number of As-species and low regulatory limits (10 μg/L) may require more than one speciation method preferably performed by species specific procedures and detectors. Beside As-fractionation for special application there are many selective speciation methods based on high performance separation techniques like capillary electrophoreses, gas and liquid chromatography. Both, fractionation and speciation methods are reviewed. However, the focus is on scopes and limits of ion chromatographic separations, the most frequently used methods. Based on IC-principles the methods applied are critically discussed and recommendations given which should result in more robust and reliable As-speciation.

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Analytical strategies for arsenic speciation in environmental and biological samples

Cited by 9 publications

References 35 publications

Closed Anaerobic Biotransformation Products of Organoarsenic Compounds inFucus distichus

Closed Anaerobic Biotransformation Products of Organoarsenic Compounds inFucus distichus

Biodegradation of arsenosugars in marine sediment

Arsenic Speciation Analysis by Ion Chromatography - A Critical Review of Principles and Applications

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