Analytical strategies for residue analysis of veterinary drugs and growth-promoting agents in food-producing animals—a review

Stolker, A.A.M.; Brinkman, U.A.Th.

doi:10.1016/j.chroma.2005.02.037

Cited by 455 publications

(262 citation statements)

References 130 publications

Supporting

Mentioning

240

Contrasting

Unclassified

Order By: Relevance

“…1,2 Estes compostos compreendem uma variedade de classes químicas com ações terapêuticas diversas, incluindo antibiótica/antimicrobiana, antiparasitária, inseticida, fungicida e sedativa. 3 Os antimicrobianos (b-lactâmicos, aminoglicosídeos e tetraciclinas) e os antiparasitários (avermectinas, piretróides e organofosforados) são os produtos mais comercializados no Paraná para tratamento do rebanho leiteiro.…”

Section: Introductionunclassified

Resíduos de medicamentos veterinários em leite e ovos

Pacheco-Silva¹,

Souza²,

Caldas³

2014

Quím. Nova

View full text Add to dashboard Cite

VETERINARY DRUG RESIDUES IN MILK AND EGGS. The use of veterinary drugs in food producing animals may result in the presence of residues in foods, including milk and eggs. Immunoassay or microbiological tests are used to screen for residues, but chromatographic methods are needed to confirm positive results. In most methods, the sample is extracted with acetonitrile, submitted to clean up or directly analyzed by LC-MS/MS. Results of the Brazilian governmental monitoring programs from 2006 to 2011 have shown that the antiparasitic ivermectin was the drug most frequently found in milk. Residues in eggs are only monitored by one of the programs, and few studies have reported the incidence of veterinary drugs in this matrix in Brazil.Keywords: veterinary drugs; milk; eggs. INTRODUÇÃOMedicamentos são usados na medicina veterinária no tratamento e prevenção de doenças ou como promotores de crescimento. 1,2 Estes compostos compreendem uma variedade de classes químicas com ações terapêuticas diversas, incluindo antibiótica/antimicrobiana, antiparasitária, inseticida, fungicida e sedativa. 3 Os antimicrobianos (b-lactâmicos, aminoglicosídeos e tetraciclinas) e os antiparasitários (avermectinas, piretróides e organofosforados) são os produtos mais comercializados no Paraná para tratamento do rebanho leiteiro. 4 O uso de medicamentos veterinários em animais produtores de alimentos pode deixar resíduos nos alimentos, como carne, leite e ovos, cujos níveis não devem ultrapassar o Limite Máximo de Resíduos (LMR). O LMR é a concentração máxima de resíduo em um alimento de origem animal resultante do uso de um medicamento veterinário (expresso em mg/kg ou µg/kg, em peso fresco) que é recomendado para ser legalmente permitido ou reconhecido como sendo aceitável no alimento. 5 Estes limites são estabelecidos para garantir o uso adequado destes produtos, limitar a exposição e proteger a saúde dos consumidores dos alimentos provenientes de animais tratados. 5 A presença de resíduos em um alimento acima do LMR estabelecido indica que o produto não foi utilizado segundo as boas práticas de uso de medicamentos veterinários (BPMV), ou o uso de um produto não autorizado. 6 O Brasil não estabelece LMR para medicamentos veterinários, adotando aqueles recomendados pelo Mercosul, Codex Alimentarius, União Européia ou Estados Unidos. 7 A exposição humana a resíduos de medicamentos veterinários presentes nos alimentos pode causar efeitos adversos, incluindo reações alérgicas em indivíduos hipersensíveis 8 e câncer. 9,10 Adicionalmente, a exposição a antimicrobianos/antibióticos pode provocar o desenvolvimento de microrganismos resistentes, que dificulta a ação terapêutica destes medicamentos em indivíduos que consumiram alimentos de animais tratados. 11,12 Resíduos de medicamentos veterinários no leite podem também causar problemas tecnológicos nos processos de fermentação dos laticínios. 13,14 No Brasil, há dois programas nacionais que monitoram a presença de resíduos de medicamentos veterinários em produtos de origem animal. O Plano Nacional...

show abstract

Section: Introductionunclassified

Resíduos de medicamentos veterinários em leite e ovos

Pacheco-Silva¹,

Souza²,

Caldas³

2014

Quím. Nova

View full text Add to dashboard Cite

show abstract

“…The residues in turn may induce allergic reactions in some hypersensitive individuals or cause the problem of drug-resistant pathogenic bacterial strains. [1][2][3] In order to address these problems, to increase food safety, and to facilitate international trade, the US Food and Drug Administration (FDA), [4] the European Union, [5] Health Canada, [6] and other international bodies [7] have established maximum residue limits (MRLs) for veterinary drug residues to monitor the level of approved veterinary drugs present in food.…”

Section: Introductionmentioning

confidence: 99%

The challenges of developing a generic extraction procedure to analyze multi‐class veterinary drug residues in milk and honey using ultra‐high pressure liquid chromatography quadrupole time‐of‐flight mass spectrometry

Wang

Leung

2012

Drug Testing and Analysis

View full text Add to dashboard Cite

This paper discusses the analytical challenges to develop a generic extraction procedure to analyze or screen multi-class veterinary drugs in milk and honey using ultra-high pressure liquid chromatography quadrupole time-of-flight mass spectrometry (UHPLC QqTOF MS). The veterinary drugs in this study included aminoglycosides, endectocides, fluoroquinolones, ionophores, b-lactams or penicillins, macrolides, NSAIDs, phenicols, sulfonamides and tetracyclines. Veterinary drugs were extracted using a QuEChERS (quick, easy, cheap, effective, rugged, and safe) method, which entailed the use of acetonitrile containing 1% acetic acid, sodium acetate, ethylenediaminetetra acetic acid disodium (EDTA) and magnesium sulfate, and no clean-up was performed. Chromatographic separation was achieved on a reversed-phase Acquity UPLC BEH C 18 , 100 Â 2.1 mm, 1.7 mm column with 0.1% formic acid and 10 mM ammonium formate in water, and acetonitrile as mobile phases. Due to poor chromatographic retention, aminoglycosides were first dropped from the list, and because of poor extractability, b-lactams and tetracyclines were also excluded from the method. The method was able to quantify 31 or screen up to 54 drugs (unbound) in honey, and to quantify 34 or screen up to 59 drugs in milk. UHPLC QqTOF data were acquired in TOF MS full-scan mode that allowed both quantification and confirmation of veterinary drugs and identification of their degradation products in samples. The method could achieve detection limits as low as 1 mg/kg with analytical range from 1 to 100 mg/kg. The developed method was intended to be used for screening of as many analytes as possible in one single analysis, or unequivocal confirmation of positive findings and degradation product identification based on accurate mass measurement and isotopic patterns.

show abstract

“…There are several review papers available outlining the extraction and purification techniques used in β-agonist (βA) analysis [210][211][212]. Target samples for residue analysis include edible tissues, plasma and urine, but also include retina and hair.…”

Section: β-Agonistsmentioning

confidence: 99%

“…Since early 2000s, LC-MS/MS has found widespread application in the analysis of anticoccidials and has allowed the simultaneous detection of their residues at low levels [212]. In addition, some groups have developed assays that additionally include nitroimidazoles [363,364].…”

Section: Anticoccidialsmentioning

confidence: 99%

Current trends in sample preparation for growth promoter and veterinary drug residue analysis

Kinsella

O’Mahony

Malone

et al. 2009

Journal of Chromatography A

181

View full text Add to dashboard Cite

A comprehensive review is presented on the current trends in sample preparation for isolation of veterinary drugs and growth promotors from foods. The objective of the review is to firstly give an overview of the sample preparation techniques that are applied in field. The review will focus on new techniques and technologies, which improve efficiency and coverage of residues. The underlying theme to the paper is the developments that have been made in multi-residue methods and particularly multi-class methods for residues of licensed animal health products, which have been developed in the last couple of years. The role of multi-class methods is discussed and how they can be accommodated in future residue surveillance.

show abstract

Analytical strategies for residue analysis of veterinary drugs and growth-promoting agents in food-producing animals—a review

Cited by 455 publications

References 130 publications

Resíduos de medicamentos veterinários em leite e ovos

Resíduos de medicamentos veterinários em leite e ovos

The challenges of developing a generic extraction procedure to analyze multi‐class veterinary drug residues in milk and honey using ultra‐high pressure liquid chromatography quadrupole time‐of‐flight mass spectrometry

Current trends in sample preparation for growth promoter and veterinary drug residue analysis

Contact Info

Product

Resources

About