A study was carried out to examine rheological properties for the detection of irradiated white pepper by viscosity and maximum viscosity methods. Samples in polyethylene bags were irradiated and measured for apparent viscosity and maximum viscosity. The viscosity of all samples decreased with increasing stirring speeds and irradiation dose. This trend was similar for maximum viscosity. The results suggest that the detection of irradiated white pepper is possible by both the viscometric and maximum viscosity methods. Therefore, maximum viscosity method can be used as a new method to detect the irradiated white pepper. radiated white pepper by viscosity and maximum viscosity methods currently used as a new method.
Materials and Methods
Material and IrradiationWhite pepper harvested in Malaysia was purchased from a local supplier. The moisture and starch content of white pepper were 13.40 Ϯ 0.70% and 0.67 g Ϯ 0.05 g/g, respectively. The moisture content was measured by the AOAC (1995) method. The starch content was determined according to the method of Hayashi and Kawashima (1982) using the enzyme reaction of glucose oxidase and peroxidase. The samples were packed in polyethylene bags and irradiated with 2.5, 5, 7.5, 10, and 15 kGy using a Co-60 irradiator (AECL, IR-79, Ontario, Canada) at the Korea Atomic Energy Research Institute. A ceric-cerous dosimeter was used to measure the exact total absorbed doses.
Measuring Method of Apparent ViscosityViscosity was measured according to the method of Hayashi and others (1996) with a slight modification. White pepper was placed in glass bottles to prepare an aqueous slurry (10%, 10 g/90 mL). After adding 2.14 mL of 33% NaOH, the samples were mixed thoroughly for 30 s. The glass bottles were heated for about 30 min in a 95 ЊC water bath with occasional stirring. The glass bottles were left in an incubator (30 ЊC) for 3 h to maintain uniform temperature. The viscosity was determined using a Brookfield DV-III equipped with an RV 6 spindle at 30 ЊC and operated at 30, 60, 90, 120, 150, 180, and 210 rpm.