Analytical study to evaluate the extracellular matrix in processed acellular xenografts

Galla, Satish; Mathapati, Santosh; Nayak, Vijaya M.; Cherian, Kotturathu Mammen; Guhathakurta, Soma

doi:10.1007/s12055-010-0026-8

Cited by 5 publications

(3 citation statements)

References 21 publications

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“…In our study, we considered in-vivo cell seeding, considering the body as the best bioreactor, and DP-processed veins were examined for cell adhesion extracellular matrix proteins such as collagen I, collagen IV, laminin, and fibronectin by immunohistochemistry. 20 These proteins were abundant although less so than in the native saphenous vein. The sheep jugular vein was chosen to observe thrombogenicity in vivo.…”

Section: Discussionmentioning

confidence: 95%

Crosslinked acellular saphenous vein for small-diameter vascular graft

Ramesh

Mathapati

Galla

et al. 2013

Asian Cardiovasc Thorac Ann

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Section: Discussionmentioning

confidence: 95%

Crosslinked acellular saphenous vein for small-diameter vascular graft

Ramesh

Mathapati

Galla

et al. 2013

Asian Cardiovasc Thorac Ann

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“…Since the luminal characteristics of the indigenous product were not changed during external coating, cell adhesion was expected to be unchanged, and it proved to have cell adhesion molecules such as collagen I and IV, laminin, and fibronectin. 12 Eventually, the coating mimics the extracellular matrix in porosity and appearance; thereby migration of cells from the blood is destined to occur in the outer layer. The inner luminal surface, which had already been evaluated for thrombogenicity and platelet aggregation and proved to be non-thrombogenic, was kept undisturbed, and only the outer surface was coated to enhance the strength.…”

Section: Discussionmentioning

confidence: 99%

Nanofiber-reinforced biological conduit in cardiac surgery: preliminary report

Guhathakurta

Galla

Ramesh

et al. 2011

Asian Cardiovasc Thorac Ann

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Several options are available for right ventricular outflow tract reconstruction, including commercially available bovine jugular vein and cryo-preserved homografts. Homograft non-availability and the problems of commercially available conduits led us to develop indigenously processed bovine jugular vein conduits with competent valves. They were made completely acellular and strengthened by non-conventional cross-linking without disturbing the extracellular matrix, which improved the luminal surface characteristics for hemocompatibility. Biocompatibility in vitro and in vivo, along with thermal stability, matrix stability, and mechanical strength have been evaluated. Sixty-nine patients received these conduits for right ventricular outflow tract reconstruction. Seven conduits dilated and 4 required replacement. To counteract dilatation, biodegradable polymeric nanofibers in various combinations and in isolation (collagen, polycaprolactone, polylactic acid) were characterized and used to reinforce the conduit circumferentially. Physical validation by mechanical testing, scanning electron microscopy, and in-vitro cytotoxicity was conducted. Thermal stability, spectroscopy studies of the polymer, and preclinical studies of the coated bovine jugular vein in animals are in progress. The feasibility studies have been completed, and the final polymer selection depends on evaluation of the functional superiority of the coated bovine jugular vein.

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“…Total GAGS were estimated by dimethyl methylene blue (DMMB) [24] assay. Total collagen content was determined by the Sircol [25] assay based on the manufacturer's protocol (Biocolor Life Sciences, UK).…”

Section: Biochemical Analysis Of Extracellular Matrixmentioning

confidence: 99%