Analytical techniques using lasers

Imasaka, Totaro; Ishibashi, Nobuhiko

doi:10.1016/0079-6727(90)90003-g

Cited by 32 publications

(9 citation statements)

References 644 publications

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“…1A and B) [32]. Since laser light intensity can be described using a Gaussian distribution there is a spatial variation in the excitation power, and therefore, the change in refractive index of the solvent is also spatially varying.…”

Section: Tls Principle Of Operationmentioning

confidence: 99%

“…Initially, intracavity and extracavity experiments employed a single laser for thermal lens generation and signal quantification. This is primarily due to the increased flexibility and sensitivity made possible by the use of discrete excitation and probe lasers, while the simplified electronics requirements are also beneficial [32]. This is primarily due to the increased flexibility and sensitivity made possible by the use of discrete excitation and probe lasers, while the simplified electronics requirements are also beneficial [32].…”

Section: Introductionmentioning

confidence: 99%

“…Such single beam TLS instruments have been successfully employed in the pioneering efforts within the field of analytical chemistry [27]; however, there has been a shift away from single beam instruments toward instruments employing a dual-beam setup [28][29][30][31], despite the increased cost and complexity. This is primarily due to the increased flexibility and sensitivity made possible by the use of discrete excitation and probe lasers, while the simplified electronics requirements are also beneficial [32]. Overall, TLS in its many embodiments has proven itself to be an incredibly useful tool.…”

Section: Introductionmentioning

confidence: 99%

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Thermal lens microscopy as a detector in microdevices

et al. 2014

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The small length scales that make microfluidics attractive are also the source of some very stringent constraints, especially with respect to the detection approach used. The low concentrations often analyzed in microfluidic devices require highly sensitive detection methods that are effective even in vanishingly small sample volumes. Over the years, many detection approaches have been developed for microfluidics. The majority of these methods rely upon optical phenomena, with the most common being fluorescence detection. Fluorescence detection is well suited to microfluidics because it is both flexible and sensitive; however, it does have shortcomings. Weak fluorescence of targets, autofluorescence of materials, and photobleaching are a few of the issues that have to be dealt with when working with fluorescence detection. Another option that eliminates all of these problems is thermal lens microscopy (TLM), a photothermal spectroscopy technique. TLM is a flexible, sensitive detection approach for nonfluorescent molecules that is capable of carrying out single-molecule detection to label-free in vivo quantification. Despite the potential benefits of TLM, it is still an underutilized detection approach. We hope this review will help broaden the use of TLM for microchip-based CE, as well as a host of other microfluidic applications.

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Section: Tls Principle Of Operationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Thermal lens microscopy as a detector in microdevices

et al. 2014

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“…Thermal lens effect has been widely used for measurement of small absorption coefficients, thermal diffusivity, and nonlinear optical properties of materials by using several techniques [3][4][5][6][7] such as thermal lens spectroscopy (TLS) and optical interferometer. In TLS, the propagation of probe beam through thermal lens will be affected resulting in a diverging or focusing of the beam center [8].…”

Section: Introductionmentioning

confidence: 99%

Calculation of Refractive Index Changes from Thermal Lens Fringes using Continuous Wavelet Algorithm

Saraç

Dursun

Yerdelen

et al. 2007

Optics & Laser Technology

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“…This laser is small and less expensive, and moreover it can be easily operated more than one year without any maintenance. The semiconductor laser is already used as a light source in fluorescence spectrometry (1,2). For example, iiisulin is determined by enzyme immunoassay (3), protein in human serum is determined after sample separation by liquid chromatography (4), etc.…”

Section: Introductionmentioning

confidence: 99%

<title>Ultratrace analysis of biological substances by capillary electrophoresis/semiconductor laser fluorometry</title>

Fuchigami¹,

Imasaka²,

Shiga³

1993

SPIE Proceedings

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Iii order to simplify a laser fluorometric detector for capillary electrophoresis, a semiconductor laser emitting at 660 nm is used as an exciting source. A labeling reagent fluorescent in the deep-red region is newly synthesized and used for determination of amino acids. The separation resolution is several hundred thousand and the detection limit is subattomole levels. Protein is also labeled by the dye developed and is measured at subfemtomole levels. Contrarily, nonfluorescent samples are detected by indirect fluorometry. Amino acids, flavin adenine dinucleotide (FAD) and deoxyadenosine monophosphate (dAMP) are determiiied, the detection limit being subpicomole levels.

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Analytical techniques using lasers

Cited by 32 publications

References 644 publications

Thermal lens microscopy as a detector in microdevices

Thermal lens microscopy as a detector in microdevices

Calculation of Refractive Index Changes from Thermal Lens Fringes using Continuous Wavelet Algorithm

<title>Ultratrace analysis of biological substances by capillary electrophoresis/semiconductor laser fluorometry</title>

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