Analytical tools for the analysis of β-carotene and its degradation products

Stutz, Hanno; Bresgen, Nikolaus; Eckl, Peter

doi:10.3109/10715762.2015.1022539

Cited by 52 publications

(39 citation statements)

References 196 publications

(426 reference statements)

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“…On the other hand, the lipid peroxidation assay with the β-carotene/linoleic acid co-oxidation system is an in vitro test that reproduces physiological situations of oxidative stress, which can lead to cell death in extreme cases by compounds that act on lipid environment (Alam et al, 2013;Stutz et al, 2015). Our data revealed that EEPR had an antioxidant potential against lipid peroxidation by inhibiting oxidative process and was more effective than rutin and quercetin.…”

Section: Ligandsmentioning

confidence: 69%

Pharmacological potential of Palicourea rigida kunth: A possible participation of flavonoid compounds

Moraes¹,

Santos²,

Fabri³

et al. 2017

J. Med. Plants Res.

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Palicourea rigida Kunth (Rubiaceae), also called "bate-caixa" or "douradão", has been used as antihypertensive, antiulcerogenic, anti-inflammatory and analgesic by traditional communities. Pharmacological potential of the ethanol extract from P. rigida (EEPR) and two quercetin derivatives were investigated. Using the high performance liquid chromatography (HPLC) assay, EEPR was analyzed. Phenolic contents (total phenolic and flavonoids) were quantified by spectrophotometric methods. 2,2-diphenyl-1-pycrilhydrazil (DPPH), iron reducing power and β-carotene/linoleic acid bleaching tests were applied to estimate the antoxidant capacity of EEPR. Nociception (acetic acidinduced writhing, formalin and hot plate) and inflammation (carrageenan-induced paw edema and pleurisy) assays were performed. Molecular docking was used to measure the interactions' profiles of ligands (rutin and quercetin) and cyclooxigenases (COX-1 and COX-2). HPLC analysis identified rutin and quercetin derivatives. Expressive levels of total phenolic and flavonoids and a promising antioxidant effect were measured. EEPR, rutin and quercetin reduced the abdominal contortions. EEPR was effective against both phases of formalin, while rutin and quercetin inhibited the second phase. The latency time on hot plate significantly increased after treatment with EEPR. Inflammatory parameters (paw edema, exudate volume and leukocyte infiltrate) were diminished by EEPR, rutin and quercetin. The molecular docking showed that rutin and quercetin are capable of complexing with COX-1 and COX-2 favorably through physical-chemical interactions. The results suggest that EEPR showed a relevant pharmacological potential, which may be related to action of rutin and quercetin derivatives.Key words: Palicourea rigida, rutin, quercetin, antioxidant, antinociception, inflammation. INTRODUCTIONIn the body, the imbalance between oxidant/antioxidant in favor of the oxidation promotes the oxidative stress that causes proteins, lipids and DNA damages, as well as induces a variety of cellular responses through the generation of reactive oxygen species (ROS) that may be originated from cellular metabolism or environmental sources (Nita and Grzybowski, 2016). ROS have become a concern among researchers because they have been associated with a significant number of diseases involving inflammatory and painful processes that affect humans and animals. In inflammation, for example, ROS cause vascular damage which allows the permeability of macromolecules and inflammatory cells from the blood to tissue (Mittal et al., 2014). This permeability is controlled by vasoactive and chemotactic mediators, which make the inflammatory process active, including the pain (Silva, 2015). Among the mediators that regulate the events of inflammation, vasoactive amines, lipid-derived eicosanoids, cytokines, chemokines and adhesion molecules have been highlighted (Silva, 2015). On the other hand, the inflammatory pain has been treated with non-steroidal anti-inflammatory agents, but present high prevalenc...

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Section: Ligandsmentioning

confidence: 69%

Pharmacological potential of Palicourea rigida kunth: A possible participation of flavonoid compounds

Moraes¹,

Santos²,

Fabri³

et al. 2017

J. Med. Plants Res.

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“…This may be attributed to the high solubility of β-carotene pigments in the powder form of herbs because grinding whole herbs affects the cell wall permeability and releases the β-carotene from the thylakoid membranes of the chloroplast. Owing to the high surface area and small particle size, the hot water extract of powder showed higher β-carotene content (Stutz, Bresgen, & Eckl, 2015).…”

Section: β-Carotene Contentmentioning

confidence: 99%

Bioactive compounds and antioxidant capacity of tea infusion prepared from whole and ground medicinal herb parts

Islam

Cho

Lee

2020

CyTA - Journal of Food

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This study was conducted to analyze bioactive compounds and the in vitro antioxidant capacity of tea infusions from whole and ground medicinal herbs, including cardamom, cinnamon, clove, nigella, and ginger. Samples of whole medicinal herbs were ground to pass through a 40 mesh (425 µm) sieve, and hot water (95°C) infusions of whole and ground herb parts were examined. Higher bioactive compounds such as phenolic compounds and flavonoids were found in the ground tea infusions. Among the medicinal herb samples, clove tea infusion showed the highest total phenol content, total flavonoid content, ABTS, and DPPH radical scavenging capacity in an average of whole and ground tea infusions. Higher β-carotene and anthocyanin content were also found in the ground nigella and cinnamon medicinal herb tea infusion than whole infusions, respectively. Therefore, ground tea infusions may be more effective in maximizing bioactive compounds and antioxidant capacity than whole infusions.Compuestos bioactivos y capacidad antioxidante de la infusión de té preparada con partes de hierbas medicinales enteras y molidas RESUMEN El presente estudio analizó los compuestos bioactivos y la capacidad antioxidante in vitro de infusiones de té preparadas con hierbas medicinales, enteras y molidas, como cardamomo, canela, clavo, nigella y jengibre. Con este objetivo se molieron muestras de hierbas medicinales completas para pasarlas a través de un tamiz de malla 40 (425 µm) y se examinaron infusiones de agua caliente (95°C) preparadas con partes de hierbas enteras y molidas. En las infusiones de té molido se encontraron mayores cantidades de compuestos bioactivos, como compuestos fenólicos y flavonoides. Para las muestras de hierbas medicinales, se comprobó que la infusión de té de clavo posee el mayor contenido total de fenol, de flavonoides, ABTS, así como la mayor capacidad de eliminación de radicales DPPH en comparación con un promedio de infusiones de té de hierbas enteras y molidas. Además, en la infusión de té de hierbas medicinales de nigella y canela molidas se encontró mayor contenido de β-caroteno y antocianina, respectivamente, que en las infusiones enteras. Por lo tanto, se concluye que las infusiones de té de hierbas molidas pueden ser más efectivas para maximizar los compuestos bioactivos y la capacidad antioxidante que las infusiones de hierbas enteras. ARTICLE HISTORY

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“…It is utilized as a food coloring agent (E number E160) [135] and also acts as a precursor and inactive form of vitamin A in nature (synthesized from carotenoids with the help of the enzyme β-carotene 15,150-monooxygenase). After biosynthesis, vitamin A conversion into retinoid takes place to prevent hypervitaminosis A [238,239].…”

Section: β-Carotenementioning

confidence: 99%

Marine Bacteria versus Microalgae: Who Is the Best for Biotechnological Production of Bioactive Compounds with Antioxidant Properties and Other Biological Applications?

Hamidi

Kozani

et al. 2019

Marine Drugs

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Natural bioactive compounds with antioxidant activity play remarkable roles in the prevention of reactive oxygen species (ROS) formation. ROS, which are formed by different pathways, have various pathological influences such as DNA damage, carcinogenesis, and cellular degeneration. Incremental demands have prompted the search for newer and alternative resources of natural bioactive compounds with antioxidant properties. The marine environment encompasses almost three-quarters of our planet and is home to many eukaryotic and prokaryotic microorganisms. Because of extreme physical and chemical conditions, the marine environment is a rich source of chemical and biological diversity, and marine microorganisms have high potential as a source of commercially interesting compounds with various pharmaceutical, nutraceutical, and cosmeceutical applications. Bacteria and microalgae are the most important producers of valuable molecules including antioxidant enzymes (such as superoxide dismutase and catalase) and antioxidant substances (such as carotenoids, exopolysaccharides, and bioactive peptides) with various valuable biological properties and applications. Here, we review the current knowledge of these bioactive compounds while highlighting their antioxidant properties, production yield, health-related benefits, and potential applications in various biological and industrial fields.

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Analytical tools for the analysis of β-carotene and its degradation products

Cited by 52 publications

References 196 publications

Pharmacological potential of Palicourea rigida kunth: A possible participation of flavonoid compounds

Pharmacological potential of Palicourea rigida kunth: A possible participation of flavonoid compounds

Bioactive compounds and antioxidant capacity of tea infusion prepared from whole and ground medicinal herb parts

Marine Bacteria versus Microalgae: Who Is the Best for Biotechnological Production of Bioactive Compounds with Antioxidant Properties and Other Biological Applications?

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