Analytical validation of an ultraviolet–visible procedure for determining vitamin D3 in vitamin D3-loaded microparticles and toxigenetic studies for incorporation into food

Silva, Tamires Barlati Vieira da; Oliveira, Anielle de; Moreira, Thaysa Fernandes Moya; Silva, Kelly Cristina da; Zanin, Rodolfo Campos; Bona, Evandro; Gonçalves, Odinei Hess; Shirai, Marianne Ayumi; Perón, Ana Paula; Leimann, Fernanda Vitória

doi:10.1016/j.foodchem.2021.129979

Cited by 6 publications

(2 citation statements)

References 39 publications

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“…The baseline was normalized by using an equal amount of empty MSs, pelleted and dissolved in chloroform. The extracted SNAP was compared with the total amount of SNAP added and the respective percentage of entrapment was calculated as reported elsewhere [ 27 ]. The SNAP-loaded MSs, free SNAP dissolved in DMSO, and water were scanned using a plate reader (Synergy H1).…”

Section: Methodsmentioning

confidence: 99%

Fabrication and Optimization of Poly(ε-caprolactone) Microspheres Loaded with S-Nitroso-N-Acetylpenicillamine for Nitric Oxide Delivery

Alvi,

Pracha,

Shalaan

et al. 2024

Biomedicines

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Heart disease is one of the leading causes of death in the United States and throughout the world. While there are different techniques for reducing or preventing the impact of heart disease, nitric oxide (NO) is administered as nitroglycerin for reversing angina or chest pain. Unfortunately, due to its gaseous and short-lived half-life, NO can be difficult to study or even administer. Therefore, controlled delivery of NO is desirable for therapeutic use. In the current study, the goal was to fabricate NO-releasing microspheres (MSs) using a donor molecule, S-Nitroso-N-Acetyl penicillamine, (SNAP), and encapsulating it in poly(ε-caprolactone) (PCL) using a single-emulsion technique that can provide sustained delivery of NO to cells over time without posing any toxicity risks. Optimization of the fabrication process was performed by varying the duration of homogenization (5, 10, and 20 min) and its effect on entrapment efficiency and size. The optimized SNAP-MS had an entrapment efficiency of ˃50%. Furthermore, we developed a modified method for NO detection by using NO microsensors to detect the NO release from SNAP-MSs in real time, showing sustained release behavior. The fabricated SNAP-MSs were tested for biocompatibility with HUVECs (human umbilical vein endothelial cells), which were found to be biocompatible. Lastly, we tested the effect of controlled NO delivery to human induced pluripotent stem-derived cardiomyocytes (hiPSC-CMs) via SNAP-MSs, which showed a significant improvement in the electrophysiological parameters and alleviated anoxic stress.

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Section: Methodsmentioning

confidence: 99%

Fabrication and Optimization of Poly(ε-caprolactone) Microspheres Loaded with S-Nitroso-N-Acetylpenicillamine for Nitric Oxide Delivery

Alvi,

Pracha,

Shalaan

et al. 2024

Biomedicines

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“…Numerous techniques have been devised to detect vitamins, including fluorescence 25 ultra-performance liquid chromatography-electrospray ionization multiple reaction monitoring/mass spectrometry (UPLC-ESIMRM/MS) 26 surface-enhanced Raman spectroscopy (SERS), 27 surface plasmon resonance (SPR), 28 capillary electrophoresis, 29 and ultraviolet-visible spectroscopy. 30 Various studies were done that used novel approaches, like Chen et al developed a novel cyanostilbene macrocycle (CSM)-based organic fluorescence sensor. By using a straightforward Schiff-base “1 + 1” condensation and a crown-ether cyanostilbene macrocycle, a significant 78% yield was obtained, which showed high turn-on green fluorescence for vitamin B1 among other guests in aqueous media.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical sensing of B-complex vitamins: current challenges and future prospects with microfluidic integration

Pakeeza,

Draz,

Yaqub

et al. 2024

RSC Adv.

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Matrix-assisted laser desorption/ionization mass spectrometry for the rapid and high throughput analysis of betaine and trigonelline in Lycium chinense Mill. and trigonelline in coffee

Zhou,

Chen,

Liu

et al. 2024

Food Chemistry: X

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Analytical validation of an ultraviolet–visible procedure for determining vitamin D3 in vitamin D3-loaded microparticles and toxigenetic studies for incorporation into food

Cited by 6 publications

References 39 publications

Fabrication and Optimization of Poly(ε-caprolactone) Microspheres Loaded with S-Nitroso-N-Acetylpenicillamine for Nitric Oxide Delivery

Fabrication and Optimization of Poly(ε-caprolactone) Microspheres Loaded with S-Nitroso-N-Acetylpenicillamine for Nitric Oxide Delivery

Electrochemical sensing of B-complex vitamins: current challenges and future prospects with microfluidic integration

Matrix-assisted laser desorption/ionization mass spectrometry for the rapid and high throughput analysis of betaine and trigonelline in Lycium chinense Mill. and trigonelline in coffee

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