Analytical Validation of Clopidogrel in Human Plasma Through Ultrahighperformance Liquid Chromatography-Tandem Mass Spectrometry

Harahap, Yahdiana; Maysyarah, Innet

doi:10.22159/ijap.2017.v9s1.79_86

Cited by 5 publications

(4 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results of REMO stability tests were summarized in table 6. The findings showed that REMO was durable in rat plasma under various storage conditions studied, including stored at Freeze-thaw at-80 °C, Bench top (normal room temperature) for 48 h, Auto-sampler at 2-8 °C for 24 h and Long term at-80 °C for 30 d [24].…”

Section: Stability Studiesmentioning

confidence: 94%

Pharmacokinetic Investigation of Remogliflozin in Rat Plasma Samples by High-Throughput HPLC-MS-MS

2022

View full text Add to dashboard Cite

Objective: Remogliflozin (REMO), a selective inhibitor of the renal sodium-dependent glucose transporter 2 channel, which could increase urine glucose excretion and lower plasma glucose in humans. To establish a simple, sensitive and completely validated HPLC-MS-MS approach for the analysis of Remogliflozin in rat plasma samples. Methods: The method was developed after simple step protein precipitation by acetonitrile and Empagliflozin (EMPA) was used as internal standard. Separation was done on an CORTECS C18, 90 Å, 2.7 µm, 4.6 mm X 150 mm with an isocratic mobile phase consisting of 0.1% Formic acid: acetonitrile (20:80%, v/v) and pumped at a flow stream of 0.8 ml/min at ambient temperature. Results: The approach developed showed fine calibration curve in the quantity range of 5-1000 pg/ml with correlation coefficient (r2) of ≥ 0.9997 and the intra-run accuracy and precision was 99.91 to 109.07% and 0.17 to 1.34, inter-run accuracy and precision was 99.8 to 101.54 and 0.17 to 1.66 according to FDA guidelines. Conclusion: The newly designed and validated approach was simple, fast and applied effectively for single-dose oral pharmacokinetic investigation in Wistar male rats for the quantification of REMO in biological matrix.

show abstract

Section: Stability Studiesmentioning

confidence: 94%

Pharmacokinetic Investigation of Remogliflozin in Rat Plasma Samples by High-Throughput HPLC-MS-MS

2022

View full text Add to dashboard Cite

show abstract

“…Chromatographic separation is based on the transfer of a liquid (mobile phase) carrying the analytical mixture through a stationary medium. The interaction difference of the analyte with the surface of the medium results in a difference in retention time between the components of the mixture [16][17][18][19][20]. UPLC differs from other chromatographic methods in the size of the column particles is substantially smaller.…”

Section: Chromatography Conditionmentioning

confidence: 99%

The Effect of Anticoagulant Types on the in Vitro Analysis of Clopidogrel in Human Plasma Using Liquid Chromatography Tandem-Mass Spectrometry

2018

Self Cite

View full text Add to dashboard Cite

Objective: The aim of this study was to optimize and validate a plasma clopidogrel analysis method using liquid chromatography tandem-mass spectrometry.Methods: Plasma samples were analyzed using a BEH C18 column (1.7 μm; 100 mm×2.1 mm), the mobile phase was 0.1% formic acid in acetonitrile (30:70, v/v). The flow rate was 0.2 mL/min, with a column temperature set to 35°C, an injection volume of 5 μL, an analysis time of 4 min, and irbesartan as the internal standard. Aliquots were obtained by liquid-liquid extraction using ammonium acetate and diethyl ether. The stability and peak area ratio of the respective plasma area responses were evaluated using ANOVA.Results: No significant differences (p>0.05) were observed between anticoagulants regarding analyte stability. However, the peak area ratio showed significant differences (p<0.05) between the anticoagulants. The accuracy and precision of the analysis with citrate, heparin, and ethylenediaminetetraacetic acid (EDTA) plasma met the quality requirements, and a linear calibration curve was created with concentrations ranging from 0.02 to 5.0 ng/mL. Conclusion:The results showed that improved analysis of clopidogrel was achieved using citrate or heparin plasma compared with EDTA plasma.

show abstract

“…After examining its effects in more than 30000 patients worldwide, the clinical advantage of long-term clopidogrel treatment in reducing atherothrombotic disorders is demonstrated [10,11]. Reviews of the literature indicate that UV spectrophotometry [12], high-performance liquid chromatography with ultraviolet detection [13], gas chromatography with mass spectrometry [14], and highperformance liquid chromatography with mass spectrometry [15,16] are used to quantitatively analyze clopidogrel in pharmaceutical preparations or biological liquids.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Study of Clopidogrel and its Determination in Pharmaceutical Preparations Using Square Wave Voltammetry

Yılmaz,

Yılmaz

2023

Cumhuriyet Science Journal

View full text Add to dashboard Cite

In the present study, the electroanalytical behaviour of clopidogrel was investigated by cyclic voltammetry method. The procedure was based on clopidogrel being electrochemically oxidized at a platinum electrode in nonaqueous solutions. At 1.93 V, the oxidation peak was noted. It was discovered that clopidogrel's oxidation was diffusion-controlled. Additionally, a quick and easy SWV approach was developed and validated in this work to determine clopidogrel in pharmaceutical preparations. At concentrations between 5 and 50 µg/mL, the calibration curve was linear. The precision was given by relative standard deviation and was less than 2.73%. Accuracy was given with relative error and did not exceed 3.89%. In pharmaceutical preparations, clopidogrel had an average recovery of 100.1%. Under the chosen experimental conditions, no interference was found. The suggested method is extremely accurate and precise. Therefore, the method is applicable to the measurement of clopidogrel in pharmaceutical formulations.

show abstract

Analytical Validation of Clopidogrel in Human Plasma Through Ultrahighperformance Liquid Chromatography-Tandem Mass Spectrometry

Cited by 5 publications

References 11 publications

Pharmacokinetic Investigation of Remogliflozin in Rat Plasma Samples by High-Throughput HPLC-MS-MS

Pharmacokinetic Investigation of Remogliflozin in Rat Plasma Samples by High-Throughput HPLC-MS-MS

The Effect of Anticoagulant Types on the in Vitro Analysis of Clopidogrel in Human Plasma Using Liquid Chromatography Tandem-Mass Spectrometry

Electrochemical Study of Clopidogrel and its Determination in Pharmaceutical Preparations Using Square Wave Voltammetry

Contact Info

Product

Resources

About