2023
DOI: 10.1002/cpz1.659
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Analyzing RNA‐Protein Interactions by Cross‐Link Rates and CLIP‐seq Libraries

Abstract: UV cross‐linking‐based methods are the most common tool to explore in vivo RNA‐protein interactions. UV cross‐linking enables the freezing of direct interactions in the cell, which can then be mapped by high‐throughput sequencing through a family of methods termed CLIP‐seq. CLIP‐seq measures the distribution of cross‐link events by purifying a protein of interest and sequencing the covalently bound RNA fragments. However, there are disagreements and ambiguities as to which proteins are RNA‐binding proteins and… Show more

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